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Literature summary extracted from

  • Teusch, M.; Forkmann, G.
    Malonyl-coenzyme A:anthocyanidin 3-glucoside malonyltransferase from flowers of Callistephus chinensis (1987), Phytochemistry, 26, 2181-2183.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.171 CaCl2 1 mM, 20% inhibition Callistephus chinensis
2.3.1.171 CoCl2 1 mM, 9% inhibition Callistephus chinensis
2.3.1.171 CuSO4 1 mM, 80% inhibition Callistephus chinensis
2.3.1.171 diethyldicarbonate 1 mM, 45% inhibition Callistephus chinensis
2.3.1.171 diethyldithiocarbamate 2 mM, 36% inhibition Callistephus chinensis
2.3.1.171 EDTA 2 mM, 34% inhibition Callistephus chinensis
2.3.1.171 FeSO4 1 mM, 45% inhibition Callistephus chinensis
2.3.1.171 KCN 1 mM, 26% inhibition Callistephus chinensis
2.3.1.171 MgCl2 1 mM, 14% inhibition Callistephus chinensis
2.3.1.171 MnCl2 1 mM, 28% inhibition Callistephus chinensis
2.3.1.171 PCMB 0.2 mM, 64% inhibition Callistephus chinensis
2.3.1.171 ZnCl2 1 mM, 37% inhibition Callistephus chinensis
2.3.1.171 ZnSO4 1 mM, 46% inhibition Callistephus chinensis

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.171 Callistephus chinensis
-
-
-

Storage Stability

EC Number Storage Stability Organism
2.3.1.171 -20°C, enzyme preparation can be stored for several weeks without appreciable loss of activity Callistephus chinensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.171 glutaryl-CoA + pelargonidin 3-O-beta-D-glucoside 50% of the activity with malonyl-CoA Callistephus chinensis CoA + pelargonidin 3-O-(6-O-glutaryl-beta-D-glucoside)
-
 ?
2.3.1.171 malonyl-CoA + cyanidin 3-O-beta-D-glucoside
-
 Callistephus chinensis CoA + cyanidin 3-O-(6-O-malonyl-beta-D-glucoside)
-
 ?
2.3.1.171 malonyl-CoA + delphinidin 3,5-di-O-beta-D-glucoside 10% of the activity with pelargonidin 3-O-glucoside Callistephus chinensis CoA + ?
-
 ?
2.3.1.171 malonyl-CoA + delphinidin 3-O-beta-D-glucoside
-
 Callistephus chinensis CoA + delphinidin 3-O-(6-O-malonyl-beta-D-glucoside)
-
 ?
2.3.1.171 malonyl-CoA + pelargonidin 3-O-beta-D-glucoside
-
 Callistephus chinensis CoA + pelargonidin 3-O-(6-O-malonyl-beta-D-glucoside)
-
 ?
2.3.1.171 methylmalonyl-CoA + pelargonidin 3-O-beta-D-glucoside 30% of the activity with malonyl-CoA Callistephus chinensis CoA + pelargonidin 3-O-(6-O-methylmalonyl-beta-D-glucoside)
-
 ?
2.3.1.171 succinyl-CoA + pelargonidin 3-O-beta-D-glucoside 40% of the activity with malonyl-CoA Callistephus chinensis CoA + pelargonidin 3-O-(6-O-succinyl-beta-D-glucoside)
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.171 50
-
-
 Callistephus chinensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.171 30 80 50% of maximal activity at 30°C and at 80°C, 0°C: 17% of maximal activity activity Callistephus chinensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.171 7
-
-
 Callistephus chinensis