EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.37 | expression in Escherichia coli | Mesembryanthemum crystallinum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.37 | oxaloacetate | - |
Mesembryanthemum crystallinum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.37 | 0.0088 | - |
oxaloacetate | pH 6.8, 30°C | Mesembryanthemum crystallinum | |
1.1.1.37 | 0.0136 | - |
NADH | pH 8.2, 30°C | Mesembryanthemum crystallinum | |
1.1.1.37 | 0.0341 | - |
L-malate | pH 8.2, 30°C | Mesembryanthemum crystallinum | |
1.1.1.37 | 0.0366 | - |
NADH | pH 6.8, 30°C | Mesembryanthemum crystallinum | |
1.1.1.37 | 0.0449 | - |
oxaloacetate | pH 8.2, 30°C | Mesembryanthemum crystallinum | |
1.1.1.37 | 0.319 | - |
NAD+ | pH 8.2, 30°C | Mesembryanthemum crystallinum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.37 | cytosol | - |
Mesembryanthemum crystallinum | 5829 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.37 | 39500 | - |
x * 39500, SDS-PAGE | Mesembryanthemum crystallinum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.37 | additional information | Mesembryanthemum crystallinum | the enzyme plays a central role in Crassulacean acid metabolism | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.37 | Mesembryanthemum crystallinum | O24047 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.37 | leaf | - |
Mesembryanthemum crystallinum | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.37 | 2-oxoglutarate + NADH + H+ | about 10% of the activity with oxaloacetate at pH 6.8 and at pH 8.2 | Mesembryanthemum crystallinum | 2-hydroxyglutarate + NAD+ | - |
? | |
1.1.1.37 | 3-bromopyruvate + NADH | about 10% of the activity with oxaloacetate at pH 6.8 | Mesembryanthemum crystallinum | 3-bromo-2-hydroxypropanoate + NAD+ | - |
? | |
1.1.1.37 | alpha-ketoisovalerate + NADH | about 10% of the activity with oxaloacetate at pH 8.2 | Mesembryanthemum crystallinum | 2-hydroxyisovalerate + NAD+ | - |
? | |
1.1.1.37 | additional information | the enzyme plays a central role in Crassulacean acid metabolism | Mesembryanthemum crystallinum | ? | - |
? | |
1.1.1.37 | additional information | less than 10% of the activity with oxaloacetate with the substrates: alpha-ketobutyrate, alpha-ketoisocaproate, alpha-ketovalerate and alpha-ketomethylvalerate | Mesembryanthemum crystallinum | ? | - |
? | |
1.1.1.37 | oxaloacetate + NADH | the ratio of oxaloacetate reduction to malate oxidation is 28.4 | Mesembryanthemum crystallinum | L-malate + NAD+ | - |
r | |
1.1.1.37 | phenylpyruvate + NADH | about 30% of the activity with oxaloacetate at pH 8.2 | Mesembryanthemum crystallinum | 2-hydroxy-3-phenylpropanoate + NAD+ | - |
? | |
1.1.1.37 | pyruvate + NADH | about 25% of the activity with oxaloacetate at pH 8.2 | Mesembryanthemum crystallinum | 2-hydroxypropanoate + NAD+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.37 | ? | x * 39500, SDS-PAGE | Mesembryanthemum crystallinum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.37 | 50 | - |
reduction of oxaloacetate | Mesembryanthemum crystallinum |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.37 | 30 | 58 | 30°C: about 50% of maximal activity, 58°C: about 55% of maximal activity | Mesembryanthemum crystallinum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.37 | 50 | - |
5 min, stable up to | Mesembryanthemum crystallinum |
1.1.1.37 | 60 | - |
5 min, about 80% loss of activity | Mesembryanthemum crystallinum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.37 | 7.5 | 9.5 | reduction of oxaloacetate | Mesembryanthemum crystallinum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.37 | NAD+ | - |
Mesembryanthemum crystallinum | |
1.1.1.37 | NADH | - |
Mesembryanthemum crystallinum |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.37 | 0.225 | - |
oxaloacetate | pH 6.8, 30°C | Mesembryanthemum crystallinum | |
1.1.1.37 | 0.775 | - |
oxaloacetate | pH 8.2, 30°C | Mesembryanthemum crystallinum |