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Literature summary extracted from

  • Busca, P.; Paradisi, F.; Moynihan, E.; Maguire, A.R.; Engel, P.C.
    Enantioselective synthesis of non-natural amino acids using phenylalanine dehydrogenases modified by site-directed mutagenesis (2004), Org. Biomol. Chem., 2, 2684-2691.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.4.1.20 N145A reduced activity with phenylpyruvate, improved activity with non-natural 2-oxo acid substrates Lysinibacillus sphaericus
1.4.1.20 N145L 22% of wild-type activity with phenylpyruvate, improved activity with non-natural 2-oxo acid substrates Lysinibacillus sphaericus
1.4.1.20 N145V reduced activity with phenylpyruvate, improved activity with non-natural 2-oxo acid substrates Lysinibacillus sphaericus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.20 phenylpyruvate + NH3 + NADH Lysinibacillus sphaericus
-
 L-phenylalanine + H2O + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.20 Lysinibacillus sphaericus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.20 2-chloro-phenylpyruvate + NH3 + NADH 7% of activity with phenylpyruvate Lysinibacillus sphaericus 2-chloro-L-phenylalanine + H2O + NAD+
-
 ?
1.4.1.20 2-fluoro-phenylpyruvate + NH3 + NADH 6.6% of activity with phenylpyruvate Lysinibacillus sphaericus 2-fluoro-L-phenylalanine + H2O + NAD+
-
 ?
1.4.1.20 3-chloro-phenylpyruvate + NH3 + NADH 0.8% of activity with phenylpyruvate Lysinibacillus sphaericus 3-chloro-L-phenylalanine + H2O + NAD+
-
 ?
1.4.1.20 3-cyclohexyl-2-oxopropionic acid + NH3 + NADH 6.1% of activity with phenylpyruvate Lysinibacillus sphaericus 3-cyclohexyl-2-aminopropionic acid + H2O + NAD+
-
 ?
1.4.1.20 3-fluoro-phenylpyruvate + NH3 + NADH 23% of activity with phenylpyruvate Lysinibacillus sphaericus 3-fluoro-L-phenylalanine + H2O + NAD+
-
 ?
1.4.1.20 4-chloro-phenylpyruvate + NH3 + NADH 62% of activity with phenylpyruvate Lysinibacillus sphaericus 4-chloro-L-phenylalanine + H2O + NAD+
-
 ?
1.4.1.20 4-fluoro-phenylpyruvate + NH3 + NADH 114% of activity with phenylpyruvate Lysinibacillus sphaericus 4-fluoro-L-phenylalanine + H2O + NAD+
-
 ?
1.4.1.20 4-methoxy-phenylpyruvate + NH3 + NADH 15% of activity with phenylpyruvate Lysinibacillus sphaericus 4-methoxy-L-phenylalanine + H2O + NAD+
-
 ?
1.4.1.20 4-methyl-phenylpyruvate + NH3 + NADH 15% of activity with phenylpyruvate Lysinibacillus sphaericus 4-methyl-L-phenylalanine + H2O + NAD+
-
 ?
1.4.1.20 4-pyridyl-phenylpyruvate + NH3 + NADH 9% of activity with phenylpyruvate Lysinibacillus sphaericus 4-pyridyl-L-phenylalanine + H2O + NAD+
-
 ?
1.4.1.20 4-trifluoromethyl-phenylpyruvate + NH3 + NADH 0.9% of activity with phenylpyruvate Lysinibacillus sphaericus 4-trifluoromethyl-L-phenylalanine + H2O + NAD+
-
 ?
1.4.1.20 alpha-ketocaproic acid + NH3 + NADH 12.8% of activity with phenylpyruvate Lysinibacillus sphaericus alpha-aminocaproic acid + H2O + NAD+
-
 ?
1.4.1.20 phenylpyruvate + NH3 + NADH
-
 Lysinibacillus sphaericus L-phenylalanine + H2O + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.1.20 PheDH
-
 Lysinibacillus sphaericus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.20 NADH
-
 Lysinibacillus sphaericus