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Literature summary extracted from
- Characterization of RNase P from Thermotoga maritima (2001), Nucleic Acids Res., 29, 880-885.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.26.5 | overexpression of the protein subunit in Escherichia coli | Thermotoga maritima |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.26.5 | 0.000223 | - |
tRNATyr precursor | pH 7.5, 50°C, enzyme RNA subunit | Thermotoga maritima |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.26.5 | KCl | activates, best at 200 mM | Thermotoga maritima |  |
| 3.1.26.5 | Mg2+ | optimal at 5-10 mM for the holoenzyme, the RNA subunit alone is active only at 100 mM MgCl2 | Thermotoga maritima | |
| 3.1.26.5 | NaCl | activates, best at 50 mM | Thermotoga maritima | |
| 3.1.26.5 | NH4Cl | activates, best at 200-400 mM | Thermotoga maritima | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.26.5 | Thermotoga maritima | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.26.5 | recombinant protein subunit from Escherichia coli, to homogeneity | Thermotoga maritima |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 3.1.26.5 | heterologous reconstitution of the holoenzyme using the RNA subunit from in vitro transcription and the recombinant protein subunit from overexpression in Escherichia coli, maximal activity at a ratio of 1:10 protein to RNA components | Thermotoga maritima |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.26.5 | tRNATyr precursor + H2O | - |
Thermotoga maritima | mature tRNATyr + 5'-terminal oligonucleotide | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.26.5 | RNase P | - |
Thermotoga maritima |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.26.5 | additional information | - |
optimal growth temperature of Thermotoga maritima is about 80°C | Thermotoga maritima |
| 3.1.26.5 | 50 | - |
about, recombinant holoenzyme | Thermotoga maritima |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.26.5 | additional information | - |
no activity at 65°C and above, recombinant holoenzyme | Thermotoga maritima |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.26.5 | 0.14 | - |
tRNATyr precursor | pH 7.5, 50°C, enzyme RNA subunit | Thermotoga maritima |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.26.5 | 7.5 | - |
recombinant holoenzyme | Thermotoga maritima |
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