Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Paetzel, M.; Danel, F.; de Castro, L.; Mosimann, S.C.; Page, M.G.; Strynadka, N.C.
    Crystal structure of the class D beta-lactamase OXA-10 (2000), Nat. Struct. Biol., 7, 918-925.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.2.6 purified recombinant native and chloride-treated enzyme, from 1.8 M ammonium sulfate, 0.1 M MES, pH 6.5, 10 mM CoCl2, vapour diffusion method, X-ray diffraction structure determination and analysis at 2.0 A resolution, modeling Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.6 Cd2+ activates 1.65fold Pseudomonas aeruginosa
3.5.2.6 Co2+ activates 1.4fold Pseudomonas aeruginosa
3.5.2.6 Cu2+ activates 1.75fold Pseudomonas aeruginosa
3.5.2.6 KCl leads to dissociation of the dimer Pseudomonas aeruginosa
3.5.2.6 Mn2+ activates slightly, 1.12fold Pseudomonas aeruginosa
3.5.2.6 additional information no effect by Ca2+, Mg2+, K+, and EDTA Pseudomonas aeruginosa
3.5.2.6 NaCl leads to dissociation of the dimer Pseudomonas aeruginosa
3.5.2.6 Ni2+ activates 1.3fold Pseudomonas aeruginosa
3.5.2.6 Zn2+ activates 1.65fold Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.6 27550
-
 2 * 27550, sequence calculation Pseudomonas aeruginosa
3.5.2.6 53000
-
 in presence of Cd2+, Cu2+ or Zn2+, gel filtration and analytical ultracentrifugation Pseudomonas aeruginosa
3.5.2.6 55100
-
 analytical ultracentriguation Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Pseudomonas aeruginosa P14489 class D beta-lactamase
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.2.6 a beta-lactam + H2O = a substituted beta-amino acid catalytic mechanism of enzyme class D, unique in comparison with classes A and C, active site structure with elements S-X-X-K, S-X-N, and K-T/S-G Pseudomonas aeruginosa
a

Subunits

EC Number Subunits Comment Organism
3.5.2.6 dimer 2 * 27550, sequence calculation Pseudomonas aeruginosa
3.5.2.6 More cation mediated formation of a homodimer, but NaCl leads to dissociation of the dimer, overview Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 OXA-10
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.6 22
-
 room temperature, assay at Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.6 7
-
 assay at Pseudomonas aeruginosa