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Literature summary extracted from

  • Pritsa, A.A.; Kyriakidis, D.A.
    L-Asparaginase of Thermus thermophilus: purification, properties and identification of essential amino acids for its catalytic activity (2001), Mol. Cell. Biochem., 216, 93-101.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.1 D-Asn competitive Thermus thermophilus
3.5.1.1 diethyldicarbonate
-
 Thermus thermophilus
3.5.1.1 iodoacetamide at pH 6.0 protection by substrate, at pH 8.0 no protection by substrate Thermus thermophilus
3.5.1.1 L-Asp competitive Thermus thermophilus
3.5.1.1 N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinline no effect up to 1 mM: Mg2+, Mn2+ and Ca2+ Thermus thermophilus
3.5.1.1 NEM
-
 Thermus thermophilus
3.5.1.1 p-hydroxymercuribenzoate
-
 Thermus thermophilus
3.5.1.1 Phenylglyoxal protection by substrate Thermus thermophilus
3.5.1.1 PMSF partial inactivation Thermus thermophilus
3.5.1.1 Zn2+ 40% inhibition by 0.5 mM, 60% inhibition by 1.0 mM Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 2.8
-
 L-Asn pH 9.2, 60°C Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.1 33000
-
 6 * 33000, SDS-PAGE Thermus thermophilus
3.5.1.1 200000
-
 gel filtration Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Thermus thermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1
-
 Thermus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.1 840
-
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-Asn + H2O
-
 Thermus thermophilus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.1 hexamer 6 * 33000, SDS-PAGE Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 9.2
-
-
 Thermus thermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.1 8.2 10.4 pH 8.2: about 60% of maximal activity, pH 10.4: about 60% of maximal activity Thermus thermophilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.1.1 Thermus thermophilus isoelectric focusing, pH-range 3-10
-
 6