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Literature summary extracted from

  • Soboh, B.; Linder, D.; Hedderich, R.
    A multisubunit membrane-bound [NiFe] hydrogenase and an NADH-dependent Fe-only hydrogenase in the fermenting bacterium Thermoanaerobacter tengcongensis (2004), Microbiology, 150, 2451-2463.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.12.1.2 FMN activates Caldanaerobacter subterraneus subsp. tengcongensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.1.2 0.017
-
 NADH 70°C Caldanaerobacter subterraneus subsp. tengcongensis
1.12.1.2 0.09
-
 NAD+ 70°C Caldanaerobacter subterraneus subsp. tengcongensis
1.12.7.2 0.003
-
 reduced ferredoxin 70°C Caldanaerobacter subterraneus subsp. tengcongensis
1.12.7.2 0.015
-
 oxidized ferredoxin 70°C Caldanaerobacter subterraneus subsp. tengcongensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.1.2 soluble
-
 Caldanaerobacter subterraneus subsp. tengcongensis
-
-
1.12.7.2 membrane membrane bound Caldanaerobacter subterraneus subsp. tengcongensis 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.1.2 Iron enzyme contains multiple iron-sulfur clusters, [4Fe-4S] or [2Fe-2S] Caldanaerobacter subterraneus subsp. tengcongensis
1.12.1.2 Ni2+ below 0.06 mol Ni2+ per mol of enzyme Caldanaerobacter subterraneus subsp. tengcongensis
1.12.7.2 Fe NiFe hydrogenase Caldanaerobacter subterraneus subsp. tengcongensis
1.12.7.2 Ni NiFe hydrogenase, contains 0.9 mol Ni per mol enzyme Caldanaerobacter subterraneus subsp. tengcongensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.1.2 14000
-
 1 * 65000 + 1 * 64000 + 1 * 20000 + 1 * 14000, SDS-PAGE Caldanaerobacter subterraneus subsp. tengcongensis
1.12.1.2 20000
-
 1 * 65000 + 1 * 64000 + 1 * 20000 + 1 * 14000, SDS-PAGE Caldanaerobacter subterraneus subsp. tengcongensis
1.12.1.2 64000
-
 1 * 65000 + 1 * 64000 + 1 * 20000 + 1 * 14000, SDS-PAGE Caldanaerobacter subterraneus subsp. tengcongensis
1.12.1.2 65000
-
 1 * 65000 + 1 * 64000 + 1 * 20000 + 1 * 14000, SDS-PAGE Caldanaerobacter subterraneus subsp. tengcongensis
1.12.1.2 162500
-
 amino acid sequence calculation Caldanaerobacter subterraneus subsp. tengcongensis
1.12.7.2 14700
-
 x * 69000 + x * 40000, + x * 32000, + x * 17000 + x + 14100 + x * 14700, SDS-PAGE Caldanaerobacter subterraneus subsp. tengcongensis
1.12.7.2 17000
-
 x * 69000 + x * 40000, + x * 32000, + x * 17000 + x + 14100 + x * 14700, SDS-PAGE Caldanaerobacter subterraneus subsp. tengcongensis
1.12.7.2 32000
-
 x * 69000 + x * 40000, + x * 32000, + x * 17000 + x + 14100 + x * 14700, SDS-PAGE Caldanaerobacter subterraneus subsp. tengcongensis
1.12.7.2 40000
-
 x * 69000 + x * 40000, + x * 32000, + x * 17000 + x + 14100 + x * 14700, SDS-PAGE Caldanaerobacter subterraneus subsp. tengcongensis
1.12.7.2 69000
-
 x * 69000 + x * 40000, + x * 32000, + x * 17000 + x + 14100 + x * 14700, SDS-PAGE Caldanaerobacter subterraneus subsp. tengcongensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.1.2 H2 + NAD+ Caldanaerobacter subterraneus subsp. tengcongensis
-
 H+ + NADH
-
 r
1.12.1.2 additional information Caldanaerobacter subterraneus subsp. tengcongensis at high H2 partial pressure enzyme activity is reduced and fermentation is partially shifted to ethanol production ?
-
 ?
1.12.7.2 H+ + reduced ferredoxin Caldanaerobacter subterraneus subsp. tengcongensis
-
 H2 + oxidized ferredoxin
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.12.1.2 Caldanaerobacter subterraneus subsp. tengcongensis
-
-
-
1.12.7.2 Caldanaerobacter subterraneus subsp. tengcongensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.1.2 20fold by several chromatographic steps Caldanaerobacter subterraneus subsp. tengcongensis
1.12.7.2 Q-sepharose HiLoad, hydroxyapatite, Superdex 200 Caldanaerobacter subterraneus subsp. tengcongensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.12.1.2 additional information activity is high in cells grown at low H2 partial pressure Caldanaerobacter subterraneus subsp. tengcongensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.1.2 0.6
-
 H2-evolution in vivo, fermenter-grown cells Caldanaerobacter subterraneus subsp. tengcongensis
1.12.1.2 5
-
 purified enzyme, H2 consumption Caldanaerobacter subterraneus subsp. tengcongensis
1.12.1.2 10
-
 purified enzyme, H2 production Caldanaerobacter subterraneus subsp. tengcongensis
1.12.1.2 1300
-
 purified enzyme, with substrates oxidized benzyl viologen and NADH Caldanaerobacter subterraneus subsp. tengcongensis
1.12.1.2 1700
-
 purified enzyme, both reaction directions with substrates oxidized/reduced methyl viologen and H2/H+ Caldanaerobacter subterraneus subsp. tengcongensis
1.12.7.2 38.5
-
 reduction of metronidiazole Caldanaerobacter subterraneus subsp. tengcongensis
1.12.7.2 391
-
 reduction of methyl viologen Caldanaerobacter subterraneus subsp. tengcongensis
1.12.7.2 1260
-
 oxidation of methyl viologen Caldanaerobacter subterraneus subsp. tengcongensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.1.2 H2 + NAD+
-
 Caldanaerobacter subterraneus subsp. tengcongensis H+ + NADH
-
 r
1.12.1.2 H2 + NAD+ electron transfer between the catalytic site for NADH-oxidation and the hydrogenase catalytic site is rate-limiting Caldanaerobacter subterraneus subsp. tengcongensis H+ + NADH
-
 r
1.12.1.2 H2 + oxidized methyl viologen
-
 Caldanaerobacter subterraneus subsp. tengcongensis H+ + reduced methyl viologen
-
 r
1.12.1.2 additional information at high H2 partial pressure enzyme activity is reduced and fermentation is partially shifted to ethanol production Caldanaerobacter subterraneus subsp. tengcongensis ?
-
 ?
1.12.1.2 additional information enzyme autoxidation Caldanaerobacter subterraneus subsp. tengcongensis ?
-
 ?
1.12.1.2 NADH + oxidized benzyl viologen
-
 Caldanaerobacter subterraneus subsp. tengcongensis NAD+ + reduced benzyl viologen
-
 r
1.12.7.2 H+ + reduced ferredoxin
-
 Caldanaerobacter subterraneus subsp. tengcongensis H2 + oxidized ferredoxin
-
 r
1.12.7.2 H+ + reduced methyl viologen
-
 Caldanaerobacter subterraneus subsp. tengcongensis H2 + oxidized methyl viologen
-
 r
1.12.7.2 H2 + ferredoxin + oxidized metronidazole
-
 Caldanaerobacter subterraneus subsp. tengcongensis H+ + ferredoxin + reduced metronidazole
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.1.2 tetramer 1 * 65000 + 1 * 64000 + 1 * 20000 + 1 * 14000, SDS-PAGE Caldanaerobacter subterraneus subsp. tengcongensis
1.12.7.2 ? x * 69000 + x * 40000, + x * 32000, + x * 17000 + x + 14100 + x * 14700, SDS-PAGE Caldanaerobacter subterraneus subsp. tengcongensis

Synonyms

EC Number Synonyms Comment Organism
1.12.1.2 NADH-dependent Fe-only hydrogenase
-
 Caldanaerobacter subterraneus subsp. tengcongensis
1.12.7.2 Ech hydrogenase
-
 Caldanaerobacter subterraneus subsp. tengcongensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.12.1.2 70
-
 assay at Caldanaerobacter subterraneus subsp. tengcongensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.1.2 FMN required, enzyme contains 1 mol FMN per mol of enzyme Caldanaerobacter subterraneus subsp. tengcongensis
1.12.1.2 additional information methyl viologen or benzyl viologen can replace NAD+/NADH Caldanaerobacter subterraneus subsp. tengcongensis
1.12.1.2 additional information no activity with NADPH/NADP+ Caldanaerobacter subterraneus subsp. tengcongensis
1.12.1.2 NAD+ highly specific for Caldanaerobacter subterraneus subsp. tengcongensis
1.12.1.2 NADH highly specific for Caldanaerobacter subterraneus subsp. tengcongensis