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Literature summary extracted from

  • Huard, C.; Miranda, G.; Wessner, F.; Bolotin, A.; Hansen, J.; Foster, S.J.; Chapot-Chartier, M.P.
    Characterization of AcmB, an N-acetylglucosaminidase autolysin from Lactococcus lactis (2003), Microbiology, 149, 695-705.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.17 acmB gene, AcmB devoid of its N-terminal domain, expression in Escherichia coli M15(pREP4), sequencing Lactococcus lactis

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.17 additional information acmB deletion/insertion mutants with lower autolysis rate/extend Lactococcus lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.17 57100
-
 x * 57100, sequence calculation Lactococcus lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.17 additional information Lactococcus lactis AcmB expression is modulated during cell growth, AcmB is not involved in cell separation but contributes to cellular autolysis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.17 Lactococcus lactis Q8KKF9 subsp. cremoris, strain MG1363
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.17 recombinant AcmB Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.17 additional information AcmB expression is modulated during cell growth, AcmB is not involved in cell separation but contributes to cellular autolysis Lactococcus lactis ?
-
 ?
3.2.1.17 peptidoglycan + H2O AcmB is an N-acetylglucosaminidase autolysin, three-domain modular structure, hydrolyzes peptidoglycans of several Gram-positive bacteria including Lactococcus lactis, AcmB hydrolyzes the peptidoglycan bonds in Bacillus subtilis HR vegetative cells between N-acetylglucosamine and N-acetylmuramic acid thus being an N-acetylglucosaminidase Lactococcus lactis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.17 ? x * 57100, sequence calculation Lactococcus lactis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.17 AcmB
-
 Lactococcus lactis
3.2.1.17 N-acetylglucosaminidase autolysin
-
 Lactococcus lactis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.17 37
-
 assay at Lactococcus lactis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.17 4
-
 assay at Lactococcus lactis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.17 additional information
-
 active at acidic pH Lactococcus lactis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.17 Lactococcus lactis sequence calculation
-
 4.9