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Literature summary extracted from

  • Nagai, M.; Kawata, M.; Watanabe, H.; Ogawa, M.; Saito, K.; Takesawa, T.; Kanda, K.; Sato, T.
    Important role of fungal intracellular laccase for melanin synthesis: purification and characterization of an intracellular laccase from Lentinula edodes fruit bodies (2003), Microbiology, 149, 2455-2462.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 HgCl2 1 mM, 67% residual activity Lentinula edodes
1.10.3.2 MnCl2 1 mM, 83% residual activity Lentinula edodes
1.10.3.2 SnCl2 1 mM, 69% residual activity Lentinula edodes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.127
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) 30°C, pH 3.0 Lentinula edodes
1.10.3.2 0.35
-
 guaiacol 30°C, pH 4.0 Lentinula edodes
1.10.3.2 0.351
-
 2,6-dimethoxyphenol 30°C, pH 3.0 Lentinula edodes
1.10.3.2 1.22
-
 beta-(3,4-dihydroxyphenyl)alanine 30°C, pH 4.0 Lentinula edodes
1.10.3.2 1.39
-
 ferulic acid 30°C, pH 5.0 Lentinula edodes
1.10.3.2 1.72
-
 catechol 30°C, pH 3.0 Lentinula edodes
1.10.3.2 1.72
-
 4-phenylenediamine 30°C, pH 4.0 Lentinula edodes
1.10.3.2 24.6
-
 pyrogallol 30°C, pH 3.0 Lentinula edodes

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 copper
-
 Lentinula edodes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 53000
-
 gel filtration Lentinula edodes
1.10.3.2 58000
-
 1 * 58000, SDS-PAGE Lentinula edodes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.10.3.2 additional information Lentinula edodes involved in melanin synthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Lentinula edodes
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.10.3.2 glycoprotein 8.6% carbohydrate content Lentinula edodes

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
 Lentinula edodes

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 fruit fully browned gill Lentinula edodes
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 305
-
 pH 3.0, 30°C Lentinula edodes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Lentinula edodes ? + H2O
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Lentinula edodes ? + H2O
-
 ?
1.10.3.2 4-phenylenediamine + O2
-
 Lentinula edodes ?
-
 r
1.10.3.2 beta-(3,4-dihydroxyphenyl)alanine + O2 i.e. L-dopa Lentinula edodes beta-(3,4-dihydroxyphenyl)alanine quinone + H2O
-
 ?
1.10.3.2 catechol + O2
-
 Lentinula edodes ? + H2O
-
 ?
1.10.3.2 ferulic acid + O2
-
 Lentinula edodes ? + H2O
-
 ?
1.10.3.2 guaiacol + O2
-
 Lentinula edodes ? + H2O
-
 ?
1.10.3.2 additional information involved in melanin synthesis Lentinula edodes ?
-
 ?
1.10.3.2 additional information no substrate: tyrosine, veratryl alcohol Lentinula edodes ?
-
 ?
1.10.3.2 p-phenylenediamine + O2
-
 Lentinula edodes ? + H2O
-
 ?
1.10.3.2 pyrogallol + O2
-
 Lentinula edodes ? + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 monomer 1 * 58000, SDS-PAGE Lentinula edodes

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 Lcc2
-
 Lentinula edodes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 40
-
-
 Lentinula edodes

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 30
-
 pH 6.0, stable for 16 h Lentinula edodes
1.10.3.2 50
-
 stable up to Lentinula edodes
1.10.3.2 60
-
 30 min, 33% loss of activity Lentinula edodes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 3
-
 substrates 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid), 2,6-dimethoxyphenol, catechol, pyrogallol Lentinula edodes
1.10.3.2 4
-
 substrates guaiacol, beta-(3,4-dihydroxyphenyl)alanine, p-phenylendiamine Lentinula edodes
1.10.3.2 5
-
 substrate ferulic acid Lentinula edodes

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 6
-
 30°C, stable for 16 h Lentinula edodes

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.10.3.2 Lentinula edodes isoelectric focusing
-
 7.1