EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.6.1.19 | citrate | 25 mM sodium citrate buffer results in 170% activity compared to 100% activity in sodium acetate buffer, both pH 5.6 | Solanum lycopersicum | |
4.6.1.19 | additional information | enzymes are induced by phosphate starvation | Solanum lycopersicum | |
4.6.1.19 | phosphate | 25 mM sodium phosphate buffer results in 153% activity compared to 100% activity in sodium acetate buffer, both pH 5.6 | Solanum lycopersicum |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.6.1.19 | - |
Arabidopsis thaliana |
4.6.1.19 | - |
Zinnia elegans |
4.6.1.19 | enzymes are S-gene products, cloning of RNases Le and LX, DNA and amino acid sequence determination and analysis | Solanum lycopersicum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.6.1.19 | 4-chloromercuribenzoic acid | 80% inhibition of RNase LE at 1 mM | Solanum lycopersicum | |
4.6.1.19 | Ag+ | complete inhibition at 10 mM | Solanum lycopersicum | |
4.6.1.19 | Al3+ | complete inhibition at 10 mM | Solanum lycopersicum | |
4.6.1.19 | Cu2+ | complete inhibition at 10 mM | Solanum lycopersicum | |
4.6.1.19 | Hg2+ | complete inhibition at 10 mM | Solanum lycopersicum | |
4.6.1.19 | additional information | no effect of 2.5 mM EDTA, no product inhibition | Solanum lycopersicum | |
4.6.1.19 | Zn2+ | complete inhibition at 10 mM | Solanum lycopersicum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.6.1.19 | endoplasmic reticulum | RNase LX | Solanum lycopersicum | 5783 | - |
4.6.1.19 | extracellular | RNase LE | Solanum lycopersicum | - |
- |
4.6.1.19 | microsome | RNase LX | Solanum lycopersicum | - |
- |
4.6.1.19 | vacuole | RNases LV1-3 | Solanum lycopersicum | 5773 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.6.1.19 | additional information | RNases LE, LX and LV-3 do not require divalent cations | Solanum lycopersicum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.6.1.19 | additional information | Solanum lycopersicum | besides other physiological functions in leaf and flower senescence and response to plant hormones, phosphate starvation, and wounding, the enzymes play a role in self-incompatibility cell-to-cell interactions | ? | - |
? | |
4.6.1.19 | additional information | Arabidopsis thaliana | enzyme plays a role in self-incompatibility cell-to-cell interactions | ? | - |
? | |
4.6.1.19 | additional information | Zinnia elegans | enzyme plays a role in self-incompatibility cell-to-cell interactions | ? | - |
? | |
4.6.1.19 | additional information | Momordica charantia | enzyme plays a role in self-incompatibility cell-to-cell interactions | ? | - |
? | |
4.6.1.19 | additional information | Nicotiana glutinosa | enzyme plays a role in self-incompatibility cell-to-cell interactions | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.6.1.19 | Arabidopsis thaliana | - |
- |
- |
4.6.1.19 | Momordica charantia | - |
bitter gourd, RNase MC1 | - |
4.6.1.19 | Nicotiana glutinosa | Q9SSV1 | RNase NGR3 | - |
4.6.1.19 | Solanum lycopersicum | - |
1 extracellular, 3 vacuolar and 1 microsomal enzyme forms | - |
4.6.1.19 | Zinnia elegans | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
4.6.1.19 | additional information | the enzymes contain secretory targeting signals, RNase LX contains an ER retention signal sequence | Solanum lycopersicum |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.6.1.19 | - |
Momordica charantia |
4.6.1.19 | - |
Nicotiana glutinosa |
4.6.1.19 | preparation of microsomes, protoplasts, vacuoles, cell culture supernatant, purification of RNase LE, LX, and LV-1-3, different methods, 1800-4400fold, overview | Solanum lycopersicum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.6.1.19 | cell suspension culture | phosphate-starved | Solanum lycopersicum | - |
4.6.1.19 | culture medium | from phosphate-starved cells | Solanum lycopersicum | - |
4.6.1.19 | leaf | - |
Nicotiana glutinosa | - |
4.6.1.19 | seed | - |
Momordica charantia | - |
EC Number | Storage Stability | Organism |
---|---|---|
4.6.1.19 | -20°C, purified enzymes, at least 2 months, no loss of activity | Solanum lycopersicum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.6.1.19 | 2',3'-cyclic NMP + H2O | slower side reaction, intrinsic cyclic nucleotide phosphodiesterase activity | Solanum lycopersicum | 3'(2')-NMP | - |
? | |
4.6.1.19 | additional information | besides other physiological functions in leaf and flower senescence and response to plant hormones, phosphate starvation, and wounding, the enzymes play a role in self-incompatibility cell-to-cell interactions | Solanum lycopersicum | ? | - |
? | |
4.6.1.19 | additional information | enzyme plays a role in self-incompatibility cell-to-cell interactions | Arabidopsis thaliana | ? | - |
? | |
4.6.1.19 | additional information | enzyme plays a role in self-incompatibility cell-to-cell interactions | Zinnia elegans | ? | - |
? | |
4.6.1.19 | additional information | enzyme plays a role in self-incompatibility cell-to-cell interactions | Momordica charantia | ? | - |
? | |
4.6.1.19 | additional information | enzyme plays a role in self-incompatibility cell-to-cell interactions | Nicotiana glutinosa | ? | - |
? | |
4.6.1.19 | additional information | enzymes show preference for release of guanine mononucleotides from diribonucleoside monophosphates or RNA, preference in descending order is G, A, U, C | Solanum lycopersicum | ? | - |
? | |
4.6.1.19 | nucleotidyl-3',5'-nuceotide + H2O | determination of base specificity | Solanum lycopersicum | 2',3'-cyclic NMP + 3'(2')-NMP | - |
? | |
4.6.1.19 | RNA + H2O | high-molecular weight fraction of RNA from Torula yeast, typeII-S | Solanum lycopersicum | 2',3'-cyclic NMP + 3'(2')-NMP | 2',3'-cyclic NMPs are are further hydrolyzed in a side reaction | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.6.1.19 | More | comparison of primary, secondary, and tertiary structures, overview | Arabidopsis thaliana |
4.6.1.19 | More | comparison of primary, secondary, and tertiary structures, overview | Solanum lycopersicum |
4.6.1.19 | More | comparison of primary, secondary, and tertiary structures, overview | Zinnia elegans |
4.6.1.19 | More | comparison of primary, secondary, and tertiary structures, overview | Momordica charantia |
4.6.1.19 | More | comparison of primary, secondary, and tertiary structures, overview | Nicotiana glutinosa |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.6.1.19 | More | enzymes are S-like ribonucleases | Arabidopsis thaliana |
4.6.1.19 | More | enzymes are S-like ribonucleases | Zinnia elegans |
4.6.1.19 | More | enzymes are S-like ribonucleases | Nicotiana glutinosa |
4.6.1.19 | More | enzymes belong to the RNase 1-type and are S-like RNases | Solanum lycopersicum |
4.6.1.19 | More | enzymes is a S-like ribonuclease | Momordica charantia |
4.6.1.19 | ribonuclease LE | - |
Solanum lycopersicum |
4.6.1.19 | RNase LE | - |
Solanum lycopersicum |
4.6.1.19 | RNase LV-1 | - |
Solanum lycopersicum |
4.6.1.19 | RNase LV-2 | - |
Solanum lycopersicum |
4.6.1.19 | RNase LV-3 | - |
Solanum lycopersicum |
4.6.1.19 | RNase LX | - |
Solanum lycopersicum |
4.6.1.19 | RNase MC1 | - |
Momordica charantia |
4.6.1.19 | RNase NGR3 | - |
Nicotiana glutinosa |
4.6.1.19 | RNase NW | - |
Nicotiana glutinosa |
4.6.1.19 | RNS1 | - |
Arabidopsis thaliana |
4.6.1.19 | RNS2 | - |
Arabidopsis thaliana |
4.6.1.19 | RNS3 | - |
Arabidopsis thaliana |
4.6.1.19 | ZRNase I | - |
Zinnia elegans |
4.6.1.19 | ZRNase II | - |
Zinnia elegans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.6.1.19 | 37 | - |
assay at | Solanum lycopersicum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.6.1.19 | 100 | - |
5 min, different pH values and ionic strengths, complete inactivation | Solanum lycopersicum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.6.1.19 | 5 | 6 | acidic optimum with yeast RNA as a substrate | Solanum lycopersicum |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
4.6.1.19 | Solanum lycopersicum | about, RNase LV-3, isoelectric focusing | - |
4 |
4.6.1.19 | Solanum lycopersicum | about, RNase LV-1, isoelectric focusing | - |
4.9 |
4.6.1.19 | Solanum lycopersicum | about, RNase LV-2, isoelectric focusing | - |
4.9 |
4.6.1.19 | Solanum lycopersicum | about, RNase LX, isoelectric focusing | - |
4.9 |