Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chang, S.T.; Parker, K.N.; Bauer, M.W.; Kelly, R.M.
    alpha-Glucosidase from Pyrococcus furiosus (2001), Methods Enzymol., 330, 260-269.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.20 nutrition usage in industrial processes to hydrolyze starch, saccharification step Pyrococcus furiosus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.20 functional overexpression in Escherichia coli Pyrococcus furiosus

General Stability

EC Number General Stability Organism
3.2.1.20 DTT and urea stabilize against thermal inactivation Pyrococcus furiosus
3.2.1.20 guanidine hydrochloride inactivates Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.20 EDTA
-
 Pyrococcus furiosus
3.2.1.20 guanidine hydrochloride inactivation Pyrococcus furiosus
3.2.1.20 SDS partial inactivation Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 additional information
-
 additional information thermal dependence of kinetics, overview Pyrococcus furiosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.20 extracellular
-
 Thermococcus sp.
-
-
3.2.1.20 intracellular
-
 Saccharolobus solfataricus 5622
-
3.2.1.20 intracellular
-
 Pyrococcus woesei 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.20 additional information no requirement for Ca2+ Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.20 60000
-
 1 * 60000 Thermococcus sp.
3.2.1.20 80000
-
 x * 80000 Saccharolobus solfataricus
3.2.1.20 125000
-
-
 Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.20 maltose + H2O Pyrococcus furiosus
-
 2 D-glucose
-
 ?
3.2.1.20 additional information Pyrococcus furiosus the enzyme catalyzes the final step in the metabolism of carbohydrates, such as starch, to glucose for cell utilization, the oligosaccharide transport system shows high affinity for trehalose and maltose ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Pyrococcus furiosus
-
 strain DSM 3638
-
3.2.1.20 Pyrococcus woesei
-
-
-
3.2.1.20 Saccharolobus solfataricus
-
-
-
3.2.1.20 Saccharolobus solfataricus 98/2
-
-
-
3.2.1.20 Thermococcus sp.
-
-
-
3.2.1.20 Thermococcus sp. AN1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20
-
 Thermococcus sp.
3.2.1.20 2 different protocols, 5-step procedure including ammonium sulfate precipitation and several chromatographic steps, 310fold Pyrococcus furiosus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.20 additional information continuous culture, growth on yeast extract, tryptone and starch Pyrococcus furiosus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 185
-
 purified enzyme, substrate methyl alpha-D-glucopyranoside Pyrococcus furiosus
3.2.1.20 287
-
 purified enzyme, substrate 4-nitrophenyl alpha-D-glucopyranoside Pyrococcus furiosus
3.2.1.20 951
-
 purified enzyme, substrate isomaltose Pyrococcus furiosus
3.2.1.20 1760
-
 purified enzyme, substrate maltose Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + alpha-D-glucopyranose
-
 ?
3.2.1.20 4-nitrophenyl alpha-D-glucopyranoside + H2O preferred substrate Thermococcus sp. 4-nitrophenol + alpha-D-glucopyranose
-
 ?
3.2.1.20 4-nitrophenyl alpha-D-glucopyranoside + H2O preferred substrate Thermococcus sp. AN1 4-nitrophenol + alpha-D-glucopyranose
-
 ?
3.2.1.20 isomaltose + H2O
-
 Pyrococcus furiosus 2 D-glucose
-
 ?
3.2.1.20 maltopentaose + H2O preferred substrate Pyrococcus woesei D-glucose + ?
-
 ?
3.2.1.20 maltose + H2O
-
 Pyrococcus furiosus 2 D-glucose
-
 ?
3.2.1.20 maltose + H2O best substrate Pyrococcus furiosus 2 D-glucose
-
 ?
3.2.1.20 maltose + H2O preferred substrate Saccharolobus solfataricus 2 D-glucose
-
 ?
3.2.1.20 maltose + H2O preferred substrate Saccharolobus solfataricus 98/2 2 D-glucose
-
 ?
3.2.1.20 methyl alpha-D-glucopyranoside + H2O
-
 Pyrococcus furiosus methanol + alpha-D-glucopyranose
-
 ?
3.2.1.20 additional information the enzyme catalyzes the final step in the metabolism of carbohydrates, such as starch, to glucose for cell utilization, the oligosaccharide transport system shows high affinity for trehalose and maltose Pyrococcus furiosus ?
-
 ?
3.2.1.20 additional information sucrose is a poor or no substrate, no activity with starch Pyrococcus furiosus ?
-
 ?
3.2.1.20 oligosaccharides + H2O various substrates, enzyme is exo-acting to release alpha-D-glucose, preferential cleavage of the alpha-1,4-bond Pyrococcus furiosus alpha-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.20 dimer 2 * 55000-60000, SDS-PAGE Pyrococcus furiosus
3.2.1.20 monomer 1 * 60000 Thermococcus sp.
3.2.1.20 tetramer or pentamer x * 80000 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 98
-
 at pH 5.0-6.0 Pyrococcus furiosus
3.2.1.20 105
-
-
 Saccharolobus solfataricus
3.2.1.20 105
-
-
 Pyrococcus woesei
3.2.1.20 105 115 at pH 5.5 Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.20 95
-
 purified enzyme, stable 3 h Thermococcus sp.
3.2.1.20 98
-
 half-life 30 min Thermococcus sp.
3.2.1.20 98
-
 half-life 160-180 min Saccharolobus solfataricus
3.2.1.20 98
-
 pH 6.5, loss of 50% activity within 48 h, in presence of 0.1 M DTT or 1 M urea 80% activity are retained for 30 min, enzyme shows loss of 78% activity in presence of 1 M SDS within 30 min, and loss of 50% activity in presence of 20 mM EDTA, complete inactivation occurs in presence of 1 M guanidine hydrochloride within 30 min Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 4.5
-
-
 Saccharolobus solfataricus
3.2.1.20 5 5.5
-
 Pyrococcus woesei
3.2.1.20 5.5
-
 assay at Pyrococcus furiosus
3.2.1.20 7.5
-
-
 Thermococcus sp.