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Literature summary extracted from

  • Iwamoto, K.; Shiraiwa, Y.
    Characterization of NADH: nitrate reductase from the coccolithophorid Emiliania huxleyi (Lohman) Hay & Mohler (Haptophyceae) (2003), Mar. Biotechnol., 5, 20-26.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.1 KCN 0.1 mM, complete inhibition Emiliania huxleyi
1.7.1.1 N-ethylmaleimide 1 mM, complete inhibition Emiliania huxleyi
1.7.1.1 NaN3 0.1 mM, complete inhibition Emiliania huxleyi
1.7.1.1 o-phenanthroline 1 mM, complete inhibition Emiliania huxleyi
1.7.1.1 p-chloromercuribenzoic acid 0.01 mM, complete inhibition Emiliania huxleyi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.1 0.04
-
 NADH pH 8.5, 25°C Emiliania huxleyi
1.7.1.1 0.104
-
 nitrate pH 8.5, 25°C Emiliania huxleyi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.1 85000
-
 6 * 85000, SDS-PAGE Emiliania huxleyi
1.7.1.1 514000
-
 gel filtration Emiliania huxleyi

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.1 Emiliania huxleyi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.1
-
 Emiliania huxleyi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.1 52.33
-
 pH 8.5, 25°C Emiliania huxleyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.1 NADH + nitrate + H+
-
 Emiliania huxleyi NAD+ + H2O + nitrite
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.1 hexamer 6 * 85000, SDS-PAGE Emiliania huxleyi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.1 8
-
-
 Emiliania huxleyi