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Literature summary extracted from

  • Deutch, C.E.
    Characterization of a salt-tolerant extracellular alpha-amylase from Bacillus dipsosauri (2002), Lett. Appl. Microbiol., 35, 78-84.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 CdCl2 30 mM, 95% inhibition Gracilibacillus dipsosauri
3.2.1.1 CoCl2 30 mM, 50% inhibition Gracilibacillus dipsosauri
3.2.1.1 maltopentaose 30 mM, 51% inhibition Gracilibacillus dipsosauri
3.2.1.1 maltotetraose 30 mM, 51% inhibition Gracilibacillus dipsosauri
3.2.1.1 maltotriose 30 mM, 42% inhibition Gracilibacillus dipsosauri
3.2.1.1 NiCl2 30 mM, 39% inhibition Gracilibacillus dipsosauri
3.2.1.1 ZnCl2 30 mM, complete inhibition Gracilibacillus dipsosauri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.1 12.5
-
 2-chloro-4-nitrophenyl-alpha-D-maltotrioside 22°C, pH 6.1 Gracilibacillus dipsosauri

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.1 extracellular
-
 Gracilibacillus dipsosauri
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.1 Na2SO4 1 M, 4fold increase in activity Gracilibacillus dipsosauri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 30000
-
 native PAGE Gracilibacillus dipsosauri
3.2.1.1 80000
-
 gel filtration Gracilibacillus dipsosauri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.1 starch + H2O Gracilibacillus dipsosauri
-
 fragments of starch
-
 ?
3.2.1.1 starch + H2O Gracilibacillus dipsosauri DD1
-
 fragments of starch
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Gracilibacillus dipsosauri
-
 moderately halophilic bacterium
-
3.2.1.1 Gracilibacillus dipsosauri DD1
-
 moderately halophilic bacterium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1 ethanol, DEAE-cellulose, BioGel P-100 Gracilibacillus dipsosauri

Storage Stability

EC Number Storage Stability Organism
3.2.1.1 4°C, 50 mM 2-[N-morpholino]ethanesulfonic acid, pH 6.1, several months, no loss of activity Gracilibacillus dipsosauri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 2-chloro-4-nitrophenyl-alpha-D-maltotrioside + H2O
-
 Gracilibacillus dipsosauri 2-chloro-4-nitrophenol + maltotriose
-
 ?
3.2.1.1 2-chloro-4-nitrophenyl-alpha-D-maltotrioside + H2O
-
 Gracilibacillus dipsosauri DD1 2-chloro-4-nitrophenol + maltotriose
-
 ?
3.2.1.1 starch + H2O
-
 Gracilibacillus dipsosauri fragments of starch
-
 ?
3.2.1.1 starch + H2O
-
 Gracilibacillus dipsosauri DD1 fragments of starch
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.1 monomer 1 * 80000, SDS-PAGE Gracilibacillus dipsosauri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 60
-
-
 Gracilibacillus dipsosauri

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.1 25 60 approx. 30% of maximal activity at 25°C, approx. 90% of maximal activity at 55°C, no activity at 65°C Gracilibacillus dipsosauri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 6.5
-
 in the presence of 1 M KCl Gracilibacillus dipsosauri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.1 5 8.5 approx. 50% of maximal activity at pH 5.5 and pH 8.0 Gracilibacillus dipsosauri