Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Patzlaff, J.S.; van der Heide, T.; Poolman, B.
    The ATP/substrate stoichiometry of the ATP-binding cassette (ABC) transporter OpuA (2003), J. Biol. Chem., 278, 29546-29551.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.6.2.9 ADP inhibits ATPase activity Lactococcus lactis
7.6.2.9 additional information the enzyme is not inhibited by 400 mM glycine betaine on the trans side of the membrane. The enzyme is not inhibited by 250 mM carnitine Lactococcus lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.6.2.9 3
-
 ATP 30ºC Lactococcus lactis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.6.2.9 membrane
-
 Lactococcus lactis 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.6.2.9 Mg2+ significant reduction of glycine betaine efflux when 6 mM ADP/Mg2+ is added to the reaction Lactococcus lactis

Organism

EC Number Organism UniProt Comment Textmining
7.6.2.9 Lactococcus lactis
-
-
-
7.6.2.9 Lactococcus lactis NZ9000
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
7.6.2.9 ATP + H2O + quaternary amine-[quaternary amine-binding protein][side 1] = ADP + phosphate + quaternary amine[side 2] + [quaternary amine-binding protein][side 1] the mechanistica stoichiometry is 2 Lactococcus lactis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.6.2.9 ATP + H2O + glycine betaine/out no efflux of glycine betaine is observed in the absence of ATP Lactococcus lactis ADP + phosphate + glycine betaine/in
-
 ?
7.6.2.9 ATP + H2O + glycine betaine/out no efflux of glycine betaine is observed in the absence of ATP Lactococcus lactis NZ9000 ADP + phosphate + glycine betaine/in
-
 ?
7.6.2.9 additional information carnitine is no substrate for the enzyme Lactococcus lactis ?
-
 ?
7.6.2.9 additional information carnitine is no substrate for the enzyme Lactococcus lactis NZ9000 ?
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
7.6.2.9 12
-
 ADP 30ºC Lactococcus lactis