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Literature summary extracted from

  • Coirini, H.; Gouezou, M.; Delespierre, B.; Schumacher, M.; Guennoun, R.
    3 beta-hydroxysteroid dehydrogenase isomerase (3beta-HSD) activity in the rat sciatic nerve: kinetic analysis and regulation by steroids (2003), J. Steroid Biochem. Mol. Biol., 85, 89-94.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.145 expression analysis of the enzyme in sciatic nerves Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.145 estradiol 0.0005 mM, 40% inhibition, 0.001 mM, 62% inhibition Rattus norvegicus
1.1.1.145 progesterone 0.025 mM, 44% inhibition, 0.05 mM, 67% inhibition Rattus norvegicus
1.1.1.145 trilostane 0.005 mM, 56% inhibition, 0.025 mM, 90% inhibition; potent inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.145 additional information
-
 additional information kinetic analysis Rattus norvegicus
1.1.1.145 0.001
-
 pregnenolone 37°C Rattus norvegicus
1.1.1.145 0.0011
-
 pregnenolone 37°C Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.145 45000
-
 x * 45000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.145 additional information Rattus norvegicus enzyme regulation by steroids, overview ?
-
 ?
1.1.1.145 pregnenolone + NAD+ Rattus norvegicus
-
 pregn-5-en-3,20-dione + NADH + H+
-
 ?
1.1.1.145 pregnenolone + NAD+ Rattus norvegicus
-
 progesterone + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.145 Rattus norvegicus
-
-
-
1.1.1.145 Rattus norvegicus
-
 male Sprague–Dawley rats, type I and IV isozymes
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.145 sciatic nerve
-
 Rattus norvegicus
-
1.1.1.145 sciatic nerve expression analysis Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.145 additional information enzyme regulation by steroids, overview Rattus norvegicus ?
-
 ?
1.1.1.145 pregnenolone + NAD+
-
 Rattus norvegicus pregn-5-en-3,20-dione + NADH + H+
-
 ?
1.1.1.145 pregnenolone + NAD+
-
 Rattus norvegicus progesterone + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.145 ? x * 45000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.145 3beta-HSD
-
 Rattus norvegicus
1.1.1.145 3beta-hydroxysteroid dehydrogenase/isomerase
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.145 37
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.145 NAD+
-
 Rattus norvegicus