Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lin, J.J.; Yang, T.H.; Wahlstrand, B.D.; Fields, P.A.; Somero, G.N.
    Phylogenetic relationships and biochemical properties of the duplicated cytosolic and mitochondrial isoforms of malate dehydrogenase from a teleost fish,Sphyraena idiastes (2002), J. Mol. Evol., 54, 107-117.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.37 expression in Escherichia coli Sphyraena idiastes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.37 0.0083
-
 oxaloacetate pH 7.5, 20°C, cytosolic isoenzyme cMDH Sus scrofa
1.1.1.37 0.0113
-
 oxaloacetate pH 7.5, 20°C, recombinant cytosolic isoenzyme cMDH-L Sphyraena idiastes
1.1.1.37 0.0113
-
 oxaloacetate isoform cMDH-S, at pH 7.5 and 20°C Sphyraena idiastes
1.1.1.37 0.0134
-
 NADH pH 7.5, 20°C, recombinant cytosolic isoenzyme cMDH-L Sphyraena idiastes
1.1.1.37 0.0134
-
 NADH isoform r-cMDH-L, at pH 7.5 and 20°C Sphyraena idiastes
1.1.1.37 0.0135
-
 oxaloacetate pH 7.5, 20°C, mitochondrial isoenzyme mMDH Sphyraena idiastes
1.1.1.37 0.0135
-
 oxaloacetate isoform r-cMDH-L, at pH 7.5 and 20°C Sphyraena idiastes
1.1.1.37 0.0145
-
 NADH pH 7.5, 20°C, mitochondrial isoenzyme mMDH Sus scrofa
1.1.1.37 0.0205
-
 NADH pH 7.5, 20°C, cytosolic isoenzyme cMDH Sus scrofa
1.1.1.37 0.0207
-
 NADH pH 7.5, 20°C, recombinant cytosolic isoenzyme cMDH-S Sphyraena idiastes
1.1.1.37 0.0207
-
 NADH isoform r-cMDH-S, at pH 7.5 and 20°C Sphyraena idiastes
1.1.1.37 0.0214
-
 NADH pH 7.5, 20°C, cytosolic isoenzyme cMDH-S Sphyraena idiastes
1.1.1.37 0.0214
-
 NADH isoform cMDH-S, at pH 7.5 and 20°C Sphyraena idiastes
1.1.1.37 0.0244
-
 NADH pH 7.5, 20°C, mitochondrial isoenzyme mMDH Sphyraena idiastes
1.1.1.37 0.0244
-
 NADH isoform r-mMDH, at pH 7.5 and 20°C Sphyraena idiastes
1.1.1.37 0.0302
-
 oxaloacetate pH 7.5, 20°C, cytosolic isoenzyme cMDH-S Sphyraena idiastes
1.1.1.37 0.0302
-
 oxaloacetate isoform r-mMDH, at pH 7.5 and 20°C Sphyraena idiastes
1.1.1.37 0.0348
-
 oxaloacetate pH 7.5, 20°C, mitochondrial isoenzyme mMDH Sus scrofa
1.1.1.37 0.039
-
 oxaloacetate pH 7.5, 20°C, recombinant cytosolic isoenzyme cMDH-S Sphyraena idiastes
1.1.1.37 0.039
-
 oxaloacetate isoform r-cMDH-S, at pH 7.5 and 20°C Sphyraena idiastes

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.37 cytosol
-
 Sphyraena idiastes 5829
-
1.1.1.37 mitochondrion
-
 Sphyraena idiastes 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.37 (S)-malate + NAD+ Sphyraena idiastes mitochondrial enzyme form mMDH functions in citric acid cycle and in the malate-aspartate shuttle oxaloacetate + NADH + H+
-
 ?
1.1.1.37 oxaloacetate + NADH Sphyraena idiastes
-
 L-malate + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.37 Sphyraena idiastes Q90YZ7 isoenzyme mMDH; Pelican barracuda
-
1.1.1.37 Sphyraena idiastes Q90YZ8 isoenzyme cMDH-L; Pelican barracuda
-
1.1.1.37 Sphyraena idiastes Q90YZ9 isoenzyme cMDH-S; Pelican barracuda
-
1.1.1.37 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.37
-
 Sphyraena idiastes

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.37 skeletal muscle white Sphyraena idiastes
-
1.1.1.37 white muscle
-
 Sphyraena idiastes
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.37 (S)-malate + NAD+ mitochondrial enzyme form mMDH functions in citric acid cycle and in the malate-aspartate shuttle Sphyraena idiastes oxaloacetate + NADH + H+
-
 ?
1.1.1.37 oxaloacetate + NADH
-
 Sus scrofa L-malate + NAD+
-
 ?
1.1.1.37 oxaloacetate + NADH
-
 Sphyraena idiastes L-malate + NAD+
-
 ?
1.1.1.37 oxaloacetate + NADH + H+
-
 Sphyraena idiastes (S)-malate + NAD+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.37 cMDH cytosolic isoenzyme Sus scrofa
1.1.1.37 cMDH -S
-
 Sphyraena idiastes
1.1.1.37 cMDH-L
-
 Sphyraena idiastes
1.1.1.37 mMDH mitochondrial isoenzyme Sus scrofa
1.1.1.37 mMDH mitochondrial isoenzyme Sphyraena idiastes

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.37 42
-
 about 20% loss of activity of cytosolic enzyme form cMDH after 50 h, about 70% loss of activity of mitochondrial enzyme form mMDH after 10 min Sus scrofa
1.1.1.37 42
-
 half-life of mMDH is about 5 min Sphyraena idiastes
1.1.1.37 42
-
 recombinant cytosolic isoenzyme c-MDH-S has a half-life of more than 500 min Sphyraena idiastes
1.1.1.37 42
-
 recombinant cytosolic isoenzyme form cMDH-L has a half-life of 3 min Sphyraena idiastes

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.37 NAD+
-
 Sphyraena idiastes
1.1.1.37 NADH
-
 Sus scrofa
1.1.1.37 NADH
-
 Sphyraena idiastes