EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.4.1.9 | A113G | mutant enzyme with altered substrate specificity. 17.9fold decrease in turnover number for L-Leu, 1.2fold decrease in turnover-number for L-Ile, 13.8fold increase in turnover number of L-norleucine, 1.7fold decrease in turnover-number for L-norvaline, 3fold decrease in turnover number for alpha-keto-isocaproate, 1.2fold decrease in turnover number for alpha-ketocaproate, 1.3fold increase in turnover number for alpha-ketocaproate, 3.6fold decrease in Km-value for L-Leu, 3.3fold increase in Km-value for L-Ile, 1.1fold decrease in Km-value for L-norleucine, 3.5fold increase in Km-value for L-norvaline, 1.9fold increase in Km-value for alpha-keto-isocaproate, 2.5fold increase in Km-value for alpha-keto-beta-methylvalerate, 2.4fold decrease in Km-value for alpha-ketocaproate, 1.2fold increase in Km-value for NAD+, 1.2fold increase in Km-value for NADH as compared to wild-type enzyme. L-Ethionine and L-Phe are not substrates of the wild-type enzyme but are deaminated by mutant enzyme. Phenylpyruvate is not a substrate of the wild-type enzyme, but is aminated by mutant enzyme | Geobacillus stearothermophilus |
1.4.1.9 | A113G/V291L | mutant enzyme with altered substrate specificity. 67.6fold decrease in turnover number for L-Leu, 20fold decrease in turnover-number for L-Ile, 2.2fold decrease in turnover number of L-norleucine, 44.8fold decrease in turnover-number for L-norvaline, 9.7fold decrease in turnover number for alpha-keto-isocaproate, 7.6fold decrease in turnover number for alpha-ketocaproate, 4.6fold decrease in turnover number for alpha-ketocaproate, 6.9fold increase in Km-value for L-Leu, 13.8fold increase in Km-value for L-Ile, 5.5fold increase in Km-value for L-norleucine, 9fold increase in Km-value for L-norvaline, 34fold increase in Km-value for alpha-keto-isocaproate, 18.2fold increase in Km-value for alpha-keto-beta-methylvalerate, 6fold increase in Km-value for alpha-ketocaproate, 4.4fold increase in Km-value for NAD+, 2fold decrease in Km-value for NADH as compared to wild-type enzyme. L-Ethionine and L-Phe are not substrates of the wild-type enzyme but are deaminated by mutant enzyme. Phenylpyruvate is not a substrate of the wild-type enzyme, but is aminated by mutant enzyme | Geobacillus stearothermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.9 | 0.017 | - |
NADH | mutant enzyme A113A/V291L | Geobacillus stearothermophilus | |
1.4.1.9 | 0.035 | - |
NADH | wild-type enzyme | Geobacillus stearothermophilus | |
1.4.1.9 | 0.042 | - |
NADH | mutant enzyme A113A | Geobacillus stearothermophilus | |
1.4.1.9 | 0.063 | - |
NAD+ | wild-type enzyme | Geobacillus stearothermophilus | |
1.4.1.9 | 0.076 | - |
NAD+ | mutant enzyme A113A | Geobacillus stearothermophilus | |
1.4.1.9 | 0.28 | - |
NAD+ | mutant enzyme A113A/V291L | Geobacillus stearothermophilus | |
1.4.1.9 | 0.88 | - |
alpha-keto-isocaproate | wild-type enzyme | Geobacillus stearothermophilus | |
1.4.1.9 | 1.4 | - |
L-Leu | mutant enzyme A113A | Geobacillus stearothermophilus | |
1.4.1.9 | 1.7 | - |
alpha-keto-isocaproate | mutant enzyme A113A | Geobacillus stearothermophilus | |
1.4.1.9 | 2 | - |
2-Oxohexanoate | mutant enzyme A113A | Geobacillus stearothermophilus | |
1.4.1.9 | 2.4 | - |
L-Ile | wild-type enzyme | Geobacillus stearothermophilus | |
1.4.1.9 | 3.8 | - |
alpha-keto-beta-methylvalerate | wild-type enzyme | Geobacillus stearothermophilus | |
1.4.1.9 | 4.1 | - |
L-norleucine | mutant enzyme A113A | Geobacillus stearothermophilus | |
1.4.1.9 | 4.4 | - |
L-norleucine | wild-type enzyme | Geobacillus stearothermophilus | |
1.4.1.9 | 4.7 | - |
2-Oxohexanoate | wild-type enzyme | Geobacillus stearothermophilus | |
1.4.1.9 | 5.1 | - |
L-Leu | wild-type enzyme | Geobacillus stearothermophilus | |
1.4.1.9 | 7.1 | - |
phenylpyruvate | mutant enzyme A113A | Geobacillus stearothermophilus | |
1.4.1.9 | 7.8 | - |
L-norvaline | wild-type enzyme | Geobacillus stearothermophilus | |
1.4.1.9 | 7.8 | - |
L-Ile | mutant enzyme A113A | Geobacillus stearothermophilus | |
1.4.1.9 | 9.4 | - |
alpha-keto-beta-methylvalerate | mutant enzyme A113A | Geobacillus stearothermophilus | |
1.4.1.9 | 9.9 | - |
phenylpyruvate | mutant enzyme A113A/V291L | Geobacillus stearothermophilus | |
1.4.1.9 | 24 | - |
L-norleucine | mutant enzyme A113A/V291L | Geobacillus stearothermophilus | |
1.4.1.9 | 27 | - |
L-norvaline | mutant enzyme A113A | Geobacillus stearothermophilus | |
1.4.1.9 | 28 | - |
2-Oxohexanoate | mutant enzyme A113A/V291L | Geobacillus stearothermophilus | |
1.4.1.9 | 30 | - |
alpha-keto-isocaproate | mutant enzyme A113A/V291L | Geobacillus stearothermophilus | |
1.4.1.9 | 31 | - |
L-Phe | mutant enzyme A113A | Geobacillus stearothermophilus | |
1.4.1.9 | 33 | - |
L-Ile | mutant enzyme A113A/V291L | Geobacillus stearothermophilus | |
1.4.1.9 | 35 | - |
L-Leu | mutant enzyme A113A/V291L | Geobacillus stearothermophilus | |
1.4.1.9 | 66 | - |
L-Phe | mutant enzyme A113A/V291L | Geobacillus stearothermophilus | |
1.4.1.9 | 69 | - |
alpha-keto-beta-methylvalerate | mutant enzyme A113A/V291L | Geobacillus stearothermophilus | |
1.4.1.9 | 70 | - |
L-norvaline | mutant enzyme A113A/V291L | Geobacillus stearothermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.9 | Geobacillus stearothermophilus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.1.9 | recombinant enzyme | Geobacillus stearothermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.9 | 2-keto-beta-methylvalerate + NH3 + NADH | as active as 2-ketoisocaproate, wild-type enzyme | Geobacillus stearothermophilus | ? + H2O + NAD+ | - |
? | |
1.4.1.9 | 2-keto-gamma-methylthiobutanoate + NH3 + NADH | 15% of the activity with 2-ketoisocaproate, wild-type enzyme | Geobacillus stearothermophilus | L-Met + H2O + NAD+ | - |
? | |
1.4.1.9 | 2-ketobutyrate + NH3 + NADH | 47% of the activity with 2-ketoisocaproate, wild-type enzyme | Geobacillus stearothermophilus | L-2-aminobutyrate + H2O + NAD+ | - |
? | |
1.4.1.9 | 2-ketoisocaproate + NH3 + NADH | - |
Geobacillus stearothermophilus | L-Ile + H2O + NAD+ | - |
? | |
1.4.1.9 | 2-ketovalerate + NH3 + NADH | 86% of the activity with 2-ketoisocaproate, wild-type enzyme | Geobacillus stearothermophilus | L-norvaline + H2O + NAD+ | - |
? | |
1.4.1.9 | 2-oxohexanoate + NH3 + NADH + H+ | - |
Geobacillus stearothermophilus | L-norleucine + H2O + NAD+ | - |
r | |
1.4.1.9 | L-Ile + H2O + NAD+ | 54% of the activity with L-Leu, wild-type enzyme | Geobacillus stearothermophilus | 3-methyl-2-oxopentanoate + NH3 + NADH | - |
? | |
1.4.1.9 | L-Leu + H2O + NAD+ | - |
Geobacillus stearothermophilus | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
? | |
1.4.1.9 | L-Met + H2O + NAD+ | 0.7% the activity with L-Leu, wild-type enzyme | Geobacillus stearothermophilus | 4-methylthio-2-oxobutyrate + NH3 + NADH + H+ | - |
? | |
1.4.1.9 | L-norleucine + H2O + NAD+ | 14% the activity with L-Leu, wild-type enzyme | Geobacillus stearothermophilus | ? + NH3 + NADH | - |
? | |
1.4.1.9 | L-norvaline + H2O + NAD+ | 56% the activity with L-Leu, wild-type enzyme | Geobacillus stearothermophilus | 2-ketovalerate + NH3 + NADH | - |
? | |
1.4.1.9 | L-Phe + H2O + NAD+ | no activity of wild-type enzyme activity with mutant enzymes A113G, A113G/V291L | Geobacillus stearothermophilus | phenylpyruvate + NH3 + NADH | - |
? | |
1.4.1.9 | L-Val + H2O + NAD+ | 39% of the activity with L-Leu, wild-type enzyme | Geobacillus stearothermophilus | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
? | |
1.4.1.9 | phenylpyruvate + NH3 + NADH | 15% of the activity with 2-ketoisocaproate, wild-type enzyme | Geobacillus stearothermophilus | L-Phe + H2O + NAD+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.1.9 | LeuDH | - |
Geobacillus stearothermophilus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.9 | 0.29 | - |
L-norvaline | mutant enzyme A113A/V291L | Geobacillus stearothermophilus | |
1.4.1.9 | 0.6 | - |
L-norleucine | mutant enzyme A113A/V291L | Geobacillus stearothermophilus | |
1.4.1.9 | 0.74 | - |
L-Leu | mutant enzyme A113A/V291L | Geobacillus stearothermophilus | |
1.4.1.9 | 0.8 | - |
L-Phe | mutant enzyme A113A | Geobacillus stearothermophilus | |
1.4.1.9 | 1.3 | - |
L-norleucine | wild-type enzyme | Geobacillus stearothermophilus | |
1.4.1.9 | 1.4 | - |
L-Phe | mutant enzyme A113A/V291L | Geobacillus stearothermophilus | |
1.4.1.9 | 1.4 | - |
L-Ile | mutant enzyme A113A/V291L | Geobacillus stearothermophilus | |
1.4.1.9 | 2 | 8 | L-Ile | wild-type enzyme | Geobacillus stearothermophilus | |
1.4.1.9 | 2.8 | - |
L-Leu | mutant enzyme A113A | Geobacillus stearothermophilus | |
1.4.1.9 | 7.7 | - |
L-norvaline | mutant enzyme A113A | Geobacillus stearothermophilus | |
1.4.1.9 | 9.9 | - |
phenylpyruvate | mutant enzyme A113A/V291L | Geobacillus stearothermophilus | |
1.4.1.9 | 13 | - |
L-norvaline | wild-type enzyme | Geobacillus stearothermophilus | |
1.4.1.9 | 18 | - |
L-norleucine | mutant enzyme A113A | Geobacillus stearothermophilus | |
1.4.1.9 | 22 | - |
phenylpyruvate | mutant enzyme A113A | Geobacillus stearothermophilus | |
1.4.1.9 | 23 | - |
L-Ile | mutant enzyme A113A | Geobacillus stearothermophilus | |
1.4.1.9 | 24 | - |
2-Oxohexanoate | mutant enzyme A113A/V291L | Geobacillus stearothermophilus | |
1.4.1.9 | 29 | - |
alpha-keto-isocaproate | mutant enzyme A113A/V291L | Geobacillus stearothermophilus | |
1.4.1.9 | 37 | - |
alpha-keto-beta-methylvalerate | mutant enzyme A113A/V291L | Geobacillus stearothermophilus | |
1.4.1.9 | 50 | - |
L-Leu | wild-type enzyme | Geobacillus stearothermophilus | |
1.4.1.9 | 94 | - |
alpha-keto-isocaproate | mutant enzyme A113A | Geobacillus stearothermophilus | |
1.4.1.9 | 110 | - |
2-Oxohexanoate | wild-type enzyme | Geobacillus stearothermophilus | |
1.4.1.9 | 140 | - |
2-Oxohexanoate | mutant enzyme A113A | Geobacillus stearothermophilus | |
1.4.1.9 | 235 | - |
alpha-keto-beta-methylvalerate | mutant enzyme A113A | Geobacillus stearothermophilus | |
1.4.1.9 | 280 | - |
alpha-keto-beta-methylvalerate | wild-type enzyme | Geobacillus stearothermophilus | |
1.4.1.9 | 280 | - |
alpha-keto-isocaproate | wild-type enzyme | Geobacillus stearothermophilus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.9 | NAD+ | - |
Geobacillus stearothermophilus | |
1.4.1.9 | NADH | - |
Geobacillus stearothermophilus |