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Literature summary extracted from

  • Katoh, R.; Nagata, S.; Misono, H.
    Cloning and sequencing of the leucine dehydrogenase gene from Bacillus sphaericus IFO 3525 and importance of the C-terminal region for the enzyme activity (2003), J. Mol. Catal. B, 23, 239-247.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.9 expression in Escherichia coli Lysinibacillus sphaericus

Protein Variants

EC Number Protein Variants Comment Organism
1.4.1.9 LeuDEL4 4 C-terminal amino acids deleted, mutant enzyme is a dimer Lysinibacillus sphaericus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.9 0.48
-
 NAD+ native enzyme Lysinibacillus sphaericus
1.4.1.9 1.25
-
 NAD+ mutant enzyme LeuDEL4 Lysinibacillus sphaericus
1.4.1.9 3.3
-
 L-Leu native enzyme Lysinibacillus sphaericus
1.4.1.9 3.8
-
 L-Val native enzyme Lysinibacillus sphaericus
1.4.1.9 11
-
 L-Leu mutant enzyme LeuDEL4 Lysinibacillus sphaericus
1.4.1.9 25
-
 L-Val mutant enzyme LeuDEL4 Lysinibacillus sphaericus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.9 43000
-
 8 * 43000, recombinant native enzyme, SDS-PAGE Lysinibacillus sphaericus
1.4.1.9 82000
-
 mutant enzyme LeuDEL4, gel filtration Lysinibacillus sphaericus
1.4.1.9 340000
-
 recombinant native enzyme, gel filtration Lysinibacillus sphaericus

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.9 Lysinibacillus sphaericus
-
 IFO 3525
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.9 recombinant enzyme Lysinibacillus sphaericus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.9 71.7
-
-
 Lysinibacillus sphaericus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.9 L-Val + H2O + NAD+
-
 Lysinibacillus sphaericus 3-methyl-2-oxobutanoate + NH3 + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.9 dimer mutant enzyme LeuDEL4 Lysinibacillus sphaericus
1.4.1.9 octamer 8 * 43000, recombinant native enzyme, SDS-PAGE Lysinibacillus sphaericus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.9 40
-
 stable up to, mutant enzyme LeuDEL4 Lysinibacillus sphaericus
1.4.1.9 55
-
 stable up to, native enzyme Lysinibacillus sphaericus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.9 10
-
 mutant enzyme LeuDEL4 Lysinibacillus sphaericus
1.4.1.9 11
-
 native enzyme Lysinibacillus sphaericus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.1.9 9.5
-
 mutant enzyme LeuDEL4 is stable Lysinibacillus sphaericus
1.4.1.9 9.5 10.5 native enzyme is stable Lysinibacillus sphaericus