EC Number | Application | Comment | Organism |
---|---|---|---|
3.6.1.23 | medicine | potential target for anti-tuberculosis therapy | Mycobacterium tuberculosis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.6.1.23 | expressed in Escherichia coli BL21-Gold (DE3) as His-tag fusion protein | Mycobacterium tuberculosis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.6.1.23 | hanging and sitting drop vapor diffusion method | Mycobacterium tuberculosis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.23 | Mg2+ | required for stabilization of the active form of the enzyme | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.23 | dUTP + H2O | Mycobacterium tuberculosis | removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA | dUMP + diphosphate | - |
? | |
3.6.1.23 | dUTP + H2O | Mycobacterium tuberculosis H37Rv | removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA | dUMP + diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.23 | Mycobacterium tuberculosis | P9WNS5 | - |
- |
3.6.1.23 | Mycobacterium tuberculosis H37Rv | P9WNS5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.6.1.23 | recombinant protein using His-tag | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.23 | dUTP + H2O | - |
Mycobacterium tuberculosis | dUMP + diphosphate | - |
? | |
3.6.1.23 | dUTP + H2O | removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA | Mycobacterium tuberculosis | dUMP + diphosphate | - |
? | |
3.6.1.23 | dUTP + H2O | - |
Mycobacterium tuberculosis H37Rv | dUMP + diphosphate | - |
? | |
3.6.1.23 | dUTP + H2O | removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA | Mycobacterium tuberculosis H37Rv | dUMP + diphosphate | - |
? |