EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.99.12 | expression in Pichia pastoris | Zea mays |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.5.99.12 | purified free recombinant enzyme, or purified recombinant enzyme complexed with trans-zeatin, N6-isopentenyladenine, and N6-benzyladenine, sitting drop vapour diffusion method, 20°C, protein solution: 30 mg/ml protein, 20 mM HEPES-KOH, pH 7.5, mixed with equal volume of well solution containing 0.1 M sodium acetat, pH 4.6, 0.2 M ammonium sulfate, and 12% w/v PEG 5000 monomethylether, a few hours, soaking in ligand solution and/or cryoprotectant 30% glycerol before data collection, X-ray diffraction structure determination and analysis at 1.7-2.0 A resolution, single isomorphous replacement, Fourier methods | Zea mays |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.99.12 | additional information | Zea mays | enzyme has a major role in control of cytokinin plant hormone levels | ? | - |
? | |
1.5.99.12 | N6-isopentenyladenine + FAD + H2O | Zea mays | oxidative degradation | adenine + 3-methylbut-2-enal + FADH2 | - |
ir |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.99.12 | Zea mays | Q9T0N8 | - |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.5.99.12 | N6-dimethylallyladenine + acceptor + H2O = adenine + 3-methylbut-2-enal + reduced acceptor | reaction scheme and mechanism, the substrate displays a plug-into-socket binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin, bipartite architecture of the catalytic centre, which consists of a funnel-shaped region on the protein surface and an internal cavity lined by the flavin ring, the 3 active site regions are connected by a pore of about 4 A diameter | Zea mays |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.99.12 | additional information | enzyme has a major role in control of cytokinin plant hormone levels | Zea mays | ? | - |
? | |
1.5.99.12 | additional information | the substrate binding site is located at amino acid residues 245-491 | Zea mays | ? | - |
? | |
1.5.99.12 | N6-benzyladenine + FAD + H2O | substrate binding mechanism and structure, overview | Zea mays | adenine + ? + FADH2 | - |
ir | |
1.5.99.12 | N6-isopentenyladenine + FAD + H2O | oxidative degradation | Zea mays | adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
1.5.99.12 | N6-isopentenyladenine + FAD + H2O | substrate binding mechanism and structure, overview | Zea mays | adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
1.5.99.12 | trans-zeatin + FAD + H2O | substrate binding mechanism and structure, overview | Zea mays | ? + FADH2 | - |
ir |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.99.12 | CKX | - |
Zea mays |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.99.12 | FAD | binding site structure comprising amino acid residues 40-244 and 492-534 | Zea mays |