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Literature summary extracted from

  • D'Amico, S.; Gerday, C.; Feller, G.
    Temperature adaptation of proteins: engineering mesophilic-like activity and stability in a cold-adapted alpha-amylase (2003), J. Mol. Biol., 332, 981-988.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.1 expression of AHA mutants in Escherichia coli Pseudoalteromonas haloplanktis

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.1 K300R 47% of wild-type kcat Pseudoalteromonas haloplanktis
3.2.1.1 K300R/N150D/V196F/Q164I/T232V 44% of wild-type kcat, increase in temperature stability Pseudoalteromonas haloplanktis
3.2.1.1 N150D 98% of wild-type kcat Pseudoalteromonas haloplanktis
3.2.1.1 Q164I 73% of wild-type kcat Pseudoalteromonas haloplanktis
3.2.1.1 T232V 105% of wild-type kcat Pseudoalteromonas haloplanktis
3.2.1.1 V196F 108% of wild-type kcat Pseudoalteromonas haloplanktis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.1 0.065
-
 4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene 25°C, pH 7.2 Sus scrofa
3.2.1.1 0.23
-
 4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene 25°C, pH 7.2 Pseudoalteromonas haloplanktis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.1 starch + H2O Sus scrofa
-
 maltooligosaccharides
-
 ?
3.2.1.1 starch + H2O Pseudoalteromonas haloplanktis
-
 maltooligosaccharides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Pseudoalteromonas haloplanktis
-
-
-
3.2.1.1 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.1 pancreas
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene + H2O
-
 Sus scrofa 4-nitrophenol + 4,6-O-ethylidene-[G7]-alpha-D-maltoheptaose
-
 ?
3.2.1.1 4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene + H2O
-
 Pseudoalteromonas haloplanktis 4-nitrophenol + 4,6-O-ethylidene-[G7]-alpha-D-maltoheptaose
-
 ?
3.2.1.1 starch + H2O
-
 Sus scrofa maltooligosaccharides
-
 ?
3.2.1.1 starch + H2O
-
 Pseudoalteromonas haloplanktis maltooligosaccharides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.1 AHA
-
 Pseudoalteromonas haloplanktis
3.2.1.1 PPA
-
 Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 45
-
 wild-type, approx. 70% loss of activity after 45 min in the absence of starch, approx. 95% loss of activity after 45 min in the presence of 2% starch, K300R/N150D/V196F/Q164I/T232V mutant, approx. 50% loss of activity in the absence of starch Pseudoalteromonas haloplanktis
3.2.1.1 60
-
 approx. 50% loss of activity after 30 min in the presence of 2% starch, approx. 85% loss of activity after 30 min in the absence of starch Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.1 141
-
 4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene 25°C, pH 7.2 Sus scrofa
3.2.1.1 291
-
 4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene 25°C, pH 7.2 Sus scrofa
3.2.1.1 392
-
 4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene 15°C, pH 7.2 Pseudoalteromonas haloplanktis
3.2.1.1 697
-
 4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene 25°C, pH 7.2 Pseudoalteromonas haloplanktis