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Literature summary extracted from

  • Chakraborti, S.; Sani, R.K.; Banerjee, U.C.; Sobti, R.C.
    Purification and characterization of a novel beta-galactosidase from Bacillus sp. MTCC 3088 (2000), J. Ind. Microbiol. Biotechnol., 24, 58-63.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 Ag+ 1-2.5 mM Bacillus sp. (in: Bacteria)
3.2.1.23 Cu2+ 1-2.5 mM Bacillus sp. (in: Bacteria)
3.2.1.23 galactose
-
 Bacillus sp. (in: Bacteria)
3.2.1.23 Hg2+ 1-2.5 mM Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 6.18
-
 lactose
-
 Bacillus sp. (in: Bacteria)
3.2.1.23 6.34
-
 o-nitrophenyl beta-D-galactopyranoside
-
 Bacillus sp. (in: Bacteria)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.23 extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.23 Mg2+ good activator Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 484000
-
 gel filtration Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Bacillus sp. (in: Bacteria)
-
 MTCC 3088
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 lactose + H2O
-
 Bacillus sp. (in: Bacteria) D-glucose + D-galactose
-
 ?
3.2.1.23 o-nitrophenyl beta-D-galactopyranoside + H2O the enzyme is specific for beta-D-anomeric linkages Bacillus sp. (in: Bacteria) o-nitrophenol + beta-D-galactose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 60
-
-
 Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 8
-
-
 Bacillus sp. (in: Bacteria)

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.23 Bacillus sp. (in: Bacteria) PAGE
-
 6