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Literature summary extracted from

  • Bernier-Villamor, L.; Navarro, E.; Sevilla, F.; Lazaro, J.J.
    Cloning and characterization of a 2-Cys peroxiredoxin from Pisum sativum (2004), J. Exp. Bot., 55, 2191-2199.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.24 expression using vector pET3d Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.24 0.0276
-
 H2O2
-
 Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.24 21913
-
 x * 21913, calculation from nucleotide sequence Pisum sativum
1.11.1.24 49000
-
 dimer, gel filtration Pisum sativum
1.11.1.24 388000
-
 decamer, gel filtration Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.24 Pisum sativum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.24
-
 Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.24 H2O2 + dithiothreitol
-
 Pisum sativum H2O + oxidized dithiothreitol
-
 ?
1.11.1.24 additional information thioredoxin m is more efficent than thioredoxin f in reducing the enzyme Pisum sativum ?
-
 ?
1.11.1.24 tert-butyl hydroperoxide + dithiothreitol
-
 Pisum sativum tert-butanol + oxidized dithiothreitol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.24 ? x * 21913, calculation from nucleotide sequence Pisum sativum
1.11.1.24 More the transition dimer-decamer produced in vitro between pH 7.5 and 8.0 suggests that a great change in the enzyme quarternary structure of the enzyme may take place in the chloroplast during the dark-light transition. The dimer-decamer equilibrium depends on NaCl concentration and concentration of dithiothreitol Pisum sativum

Synonyms

EC Number Synonyms Comment Organism
1.11.1.24 2-Cys peroxiredoxin
-
 Pisum sativum
1.11.1.24 2-Cys Prx
-
 Pisum sativum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.24 0.69
-
 H2O2
-
 Pisum sativum

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.24 thioredoxin thioredoxin m is more efficent than thioredoxin f in reducing the enzyme Pisum sativum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.24 Pisum sativum calculation from nucleotide sequence
-
 4.9