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Literature summary extracted from

  • Ter Kuile, B.H.; Hrdy, I.; Sanchez, L.B.; Muller, M.
    Purification and specificity of two alpha-glucosidase isoforms of the parasitic protist Trichomonas vaginalis (2000), J. Eukaryot. Microbiol., 47, 440-442.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.20 additional information no inhibition by phosphate Trichomonas vaginalis
3.2.1.20 Naphthyl acetate
-
 Trichomonas vaginalis
3.2.1.20 p-chloromercuribenzoic acid
-
 Trichomonas vaginalis
3.2.1.20 wheat amylase inhibitor
-
 Trichomonas vaginalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 0.4
-
 maltose isozyme 1, pH 4.5, 37°C Trichomonas vaginalis
3.2.1.20 2
-
 maltose isozyme 2, pH 5.5, 37°C Trichomonas vaginalis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Trichomonas vaginalis
-
 parasitic protist, 2 isozymes 1 and 2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20 2 isozymes 1 and 2, isozyme 2 is purified 472fold Trichomonas vaginalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 7.9
-
 purified isozyme 1, substrate 4-nitrophenyl alpha-D-glucopyranoside Trichomonas vaginalis
3.2.1.20 17
-
 purified isozyme 2, substrate 4-nitrophenyl alpha-D-glucopyranoside Trichomonas vaginalis
3.2.1.20 30
-
 purified isozyme 1, substrate maltose Trichomonas vaginalis
3.2.1.20 101
-
 purified isozyme 2, substrate maltose Trichomonas vaginalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Trichomonas vaginalis 4-nitrophenol + alpha-D-glucopyranose
-
 ?
3.2.1.20 isomaltose + H2O
-
 Trichomonas vaginalis 2 alpha-D-glucose
-
 ?
3.2.1.20 maltoheptaose + H2O
-
 Trichomonas vaginalis maltohexaose + D-glucose
-
 ?
3.2.1.20 maltohexaose + H2O
-
 Trichomonas vaginalis maltopentaose + D-glucose
-
 ?
3.2.1.20 maltopentaose + H2O
-
 Trichomonas vaginalis alpha-D-glucose + ?
-
 ?
3.2.1.20 maltose + H2O preferred substrate of both isozymes Trichomonas vaginalis alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 maltotetraose + H2O
-
 Trichomonas vaginalis alpha-D-glucose
-
 ?
3.2.1.20 maltotriose + H2O
-
 Trichomonas vaginalis maltose + alpha-D-glucose
-
 ?
3.2.1.20 additional information substrate specificity, no activity of either isozyme with amylose and glycogen, no activity of isozyme 1 with amylopectin, starch, trehalose, panose, and lactose are a poor substrates for both isozymes, amylopectin is a poor substrate for isozyme 2 Trichomonas vaginalis ?
-
 ?
3.2.1.20 nigerose + H2O high activity Trichomonas vaginalis 2 alpha-D-glucose
-
 ?
3.2.1.20 turanose + H2O
-
 Trichomonas vaginalis alpha-D-glucose + D-fructose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.20 ? x * 100000-105000, isozymes 1 and 2, SDS-PAGE Trichomonas vaginalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 4.5
-
 isozyme 1 Trichomonas vaginalis
3.2.1.20 5.5
-
 isozyme 2 Trichomonas vaginalis