Any feedback?
Literature summary extracted from
- Investigation of glucose 6-phosphate dehydrogenase (G6PD) kinetics for normal and G6PD-deficient persons and the effects of some drugs (2004), J. Enzyme Inhib. Med. Chem., 19, 45-50.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.49 | metamizol | activation of mutant enzymes 1 and 2 | Homo sapiens |  |
| 1.1.1.49 | streptomycin | activation of mutant enzymes 1 and 2 | Homo sapiens | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.49 | metamizol | inhibition of wild-type and mutant enzyme 3 | Homo sapiens | |
| 1.1.1.49 | netilmicin | inhibition of wild-type and mutant enzymes 1-3 | Homo sapiens | |
| 1.1.1.49 | sodium ceftizoxime | inhibition of wild-type and mutant enzymes 1-3 | Homo sapiens | |
| 1.1.1.49 | sodium cefuroxime | inhibition of wild-type and mutant enzymes 1-3 | Homo sapiens | |
| 1.1.1.49 | streptomycin | inhibition of wild-type and mutant enzyme 3 | Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.49 | 0.048 | - |
D-glucose 6-phosphate | pH 8.0, 25, mutant 2 | Homo sapiens | |
| 1.1.1.49 | 0.07 | - |
NADP+ | pH 8.0, 25, mutant enzyme 2 | Homo sapiens | |
| 1.1.1.49 | 0.083 | - |
D-glucose 6-phosphate | pH 8.0, 25, mutant 3 | Homo sapiens | |
| 1.1.1.49 | 0.085 | - |
NADP+ | pH 8.0, 25, mutant enzyme 3 | Homo sapiens | |
| 1.1.1.49 | 0.13 | - |
NADP+ | pH 8.0, 25, mutant enzyme 1 | Homo sapiens | |
| 1.1.1.49 | 0.15 | - |
D-glucose 6-phosphate | pH 8.0, 25, mutant 1 | Homo sapiens | |
| 1.1.1.49 | 0.21 | - |
NADP+ | pH 8.0, 25, wild-type enzyme | Homo sapiens | |
| 1.1.1.49 | 0.5 | - |
D-glucose 6-phosphate | pH 8.0, 25, wild-type enzyme | Homo sapiens | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.49 | Mg2+ | - |
Homo sapiens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.49 | D-glucose 6-phosphate + NADP+ | Homo sapiens | key enzyme catalyzing the first step in the pentose phosphate pathway | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.49 | Homo sapiens | - |
wild-type and 3 enzyme-deficient children, i.e. mutants 1,2, and 3 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.49 | wild-type enzyme, 4.1fold from blood by ammonium sulfate precipitation, dialysis, and 2',5'-ADP affinity chromatography | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.49 | blood | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.49 | 27.5 | - |
purified wild-type enzyme | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.49 | D-glucose 6-phosphate + NADP+ | - |
Homo sapiens | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? | |
| 1.1.1.49 | D-glucose 6-phosphate + NADP+ | key enzyme catalyzing the first step in the pentose phosphate pathway | Homo sapiens | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.49 | ? | 59000-60000, wild-type and mutant enzymes, SDS-PAGE | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.49 | G6PD | - |
Homo sapiens |
| 1.1.1.49 | glucose-6-phosphate dehydrogenase | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.49 | 37 | - |
wild-type enzyme | Homo sapiens |
| 1.1.1.49 | 40 | - |
mutant enzymes 1 and 3 | Homo sapiens |
| 1.1.1.49 | 45 | - |
mutant enzyme 2 | Homo sapiens |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.49 | additional information | - |
mutant enzymes are more thermostable than the wild-type enzyme | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.49 | 8 | - |
wild-type enzyme | Homo sapiens |
| 1.1.1.49 | 8.5 | - |
mutant enzymes 1 and 3 | Homo sapiens |
| 1.1.1.49 | 9 | - |
mutant enzyme 2 | Homo sapiens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.49 | 6 | 9 | - |
Homo sapiens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.49 | NADP+ | - |
Homo sapiens | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.49 | additional information | - |
additional information | - |
Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
