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Literature summary extracted from

  • Satoh, Y.; Tajima, K.; Tannai, H.; Munekata, M.
    Enzyme-catalyzed poly(3-hydroxybutyrate) synthesis from acetate with CoA recycling and NADPH regeneration in vitro (2003), J. Biosci. Bioeng., 95, 335-341.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.36 synthesis establishing of an enzyme-catalyzed synthesis system for production of poly(3-hydroxybutyrate) in vitro on the basis of the poly(3-hydroxybutyrate) biosynthesis pathway of Ralstonia eutropha, recycling CoA for synthesis of acetyl-CoA and deriving NADPH from regeneration by GDH, overview Cupriavidus necator
1.1.1.119 synthesis use of enzyme in enzyme-catalyzed synthesis system for poly(3-hydroxybutyrate), enzyme catalyzes regeneration of NADPH, system yields 5.6 mg of poly(3-hydroxybutyrate), in a 5 ml-reaction mixture Cupriavidus necator

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.36 gene phaB, expression of the N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Cupriavidus necator

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.36 NADP+ competitive inhibition Cupriavidus necator

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.36 Cupriavidus necator
-
-
-
1.1.1.119 Cupriavidus necator
-
 recombinant enzyme, enzyme-based synthesis system for poly(3-hydroxybutyrate)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.36 recombinant His-tagged enzyme from Escherichia coli by nickel-affinity chromatography Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.36 acetoacetyl-CoA + NADPH + H+ step in the enzyme-catalyzed synthesis system for production of poly(3-hydroxybutyrate) in vitro, overview Cupriavidus necator (R)-3-hydroxybutyryl-CoA + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.36 NADPH-dependent acetoacetyl-CoA reductase
-
 Cupriavidus necator
1.1.1.36 PhaB
-
 Cupriavidus necator

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.36 25
-
 assay at Cupriavidus necator

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.36 7.5 8 assay at Cupriavidus necator

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.36 NADPH dependent on Cupriavidus necator