Literature summary extracted from
Satoh, Y.; Tajima, K.; Tannai, H.; Munekata, M.
Enzyme-catalyzed poly(3-hydroxybutyrate) synthesis from acetate with CoA recycling and NADPH regeneration in vitro (2003), J. Biosci. Bioeng., 95, 335-341.
Application
EC Number |
Application |
Comment |
Organism |
---|
1.1.1.36 |
synthesis |
establishing of an enzyme-catalyzed synthesis system for production of poly(3-hydroxybutyrate) in vitro on the basis of the poly(3-hydroxybutyrate) biosynthesis pathway of Ralstonia eutropha, recycling CoA for synthesis of acetyl-CoA and deriving NADPH from regeneration by GDH, overview |
Cupriavidus necator |
1.1.1.119 |
synthesis |
use of enzyme in enzyme-catalyzed synthesis system for poly(3-hydroxybutyrate), enzyme catalyzes regeneration of NADPH, system yields 5.6 mg of poly(3-hydroxybutyrate), in a 5 ml-reaction mixture |
Cupriavidus necator |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.1.1.36 |
gene phaB, expression of the N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) |
Cupriavidus necator |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
1.1.1.36 |
NADP+ |
competitive inhibition |
Cupriavidus necator |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.1.1.36 |
Cupriavidus necator |
- |
- |
- |
1.1.1.119 |
Cupriavidus necator |
- |
recombinant enzyme, enzyme-based synthesis system for poly(3-hydroxybutyrate) |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.1.1.36 |
recombinant His-tagged enzyme from Escherichia coli by nickel-affinity chromatography |
Cupriavidus necator |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.1.1.36 |
acetoacetyl-CoA + NADPH + H+ |
step in the enzyme-catalyzed synthesis system for production of poly(3-hydroxybutyrate) in vitro, overview |
Cupriavidus necator |
(R)-3-hydroxybutyryl-CoA + NADP+ |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.1.1.36 |
NADPH-dependent acetoacetyl-CoA reductase |
- |
Cupriavidus necator |
1.1.1.36 |
PhaB |
- |
Cupriavidus necator |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
1.1.1.36 |
25 |
- |
assay at |
Cupriavidus necator |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.1.1.36 |
7.5 |
8 |
assay at |
Cupriavidus necator |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.1.1.36 |
NADPH |
dependent on |
Cupriavidus necator |
|