EC Number | General Stability | Organism |
---|---|---|
1.1.3.6 | stable in nonionic detergents over a wide range. More than 90% of activity remains after 60 min at 60°C, pH 7.0, at concentrations of 0.1% Emulgen A-60, Triton X-100, Triton X-405 or sodium cholate | gamma proteobacterium Y-134 |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.3.6 | 2-pyridylhydroxymethane sulfate | 1 mM, 30 min at 4°C, 34% inhibition | gamma proteobacterium Y-134 | |
1.1.3.6 | 8-hydroxyquinoline | 1 mM, 30 min at 4°C, 29% inhibition | gamma proteobacterium Y-134 | |
1.1.3.6 | FAD | 1 mM, 30 min at 4°C, 9% inhibition | gamma proteobacterium Y-134 | |
1.1.3.6 | FMN | 1 mM, 30 min at 4°C, 11% inhibition | gamma proteobacterium Y-134 | |
1.1.3.6 | o-phenanthroline | 1 mM, 30 min at 4°C, 30% inhibition | gamma proteobacterium Y-134 | |
1.1.3.6 | potassium cyanide | 1 mM, 30 min at 4°C, 12% inhibition | gamma proteobacterium Y-134 |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.3.6 | MgCl2 | 1 mM, 30 min at 4°C, activation to 113% of control | gamma proteobacterium Y-134 | |
1.1.3.6 | MnCl2 | 1 mM, 30 min at 4°C, 35% inhibition | gamma proteobacterium Y-134 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.3.6 | gamma proteobacterium Y-134 | - |
uncultured | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.3.6 | - |
gamma proteobacterium Y-134 |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.3.6 | additional information | enzyme production reaches its maximum by incubation at 30°C for 12 d | gamma proteobacterium Y-134 | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.3.6 | 14.4 | - |
- |
gamma proteobacterium Y-134 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.6 | 7-dehydrocholesterol + O2 | 14% of the activity with cholesterol | gamma proteobacterium Y-134 | cholest-4,7-dien-3-one + H2O2 | - |
? | |
1.1.3.6 | beta-sitosterol + O2 | 29% of the activity with cholesterol | gamma proteobacterium Y-134 | stigmast-5-en-3-one + H2O2 | - |
? | |
1.1.3.6 | cholesterol + O2 | - |
gamma proteobacterium Y-134 | cholest-4-en-3-one + H2O2 | - |
? | |
1.1.3.6 | dihydrocholesterol + O2 | 79% of the activity with cholesterol | gamma proteobacterium Y-134 | 5alpha-cholestan-3-one + H2O2 | - |
? | |
1.1.3.6 | additional information | no activity with: ergosterol, dehydroepiandrosterone, epiandrosterone, pregnenolone | gamma proteobacterium Y-134 | ? | - |
? | |
1.1.3.6 | stigmastanol + O2 | 15% of the activity with cholesterol | gamma proteobacterium Y-134 | stigmastanone + H2O2 | - |
? | |
1.1.3.6 | stigmasterol + O2 | 32% of the activity with cholesterol | gamma proteobacterium Y-134 | stigmasta-5,22-dien-3-one + H2O2 | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.3.6 | 50 | - |
- |
gamma proteobacterium Y-134 |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.3.6 | 30 | 70 | 30°C: about 85% of maximal activity, 70°C: about 80% of maximal activity | gamma proteobacterium Y-134 |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.3.6 | 60 | - |
pH 7.0, 60 min, about 10% loss of activity | gamma proteobacterium Y-134 |
1.1.3.6 | 70 | - |
pH 7.0, 10 min, about 55% loss of activity | gamma proteobacterium Y-134 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.6 | 6.5 | - |
- |
gamma proteobacterium Y-134 |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.6 | 5 | 7.5 | pH 5.0: about 70% of maximal activity, pH 7.5: about 30% of maximal activity | gamma proteobacterium Y-134 |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.6 | 5 | 6.5 | 60°C, 60 min, stable | gamma proteobacterium Y-134 |
1.1.3.6 | 7.5 | - |
60°C, 60 min, about 10% loss of activity | gamma proteobacterium Y-134 |
1.1.3.6 | 8 | - |
60°C, 60 min, about 40% loss of activity | gamma proteobacterium Y-134 |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.1.3.6 | gamma proteobacterium Y-134 | isoelectric focusing, pH-gradient 3.5-10.0 | - |
4.2 |