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Literature summary extracted from

  • Isobe, K.; Shoji, K.; Nakanishi, Y.; Yokoe, M.; Wakao, N.
    Purification and some properties of cholesterol oxidase stable in detergents from gamma-Proteobacterium Y-134 (2003), J. Biosci. Bioeng., 95, 257-263.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.3.6 stable in nonionic detergents over a wide range. More than 90% of activity remains after 60 min at 60°C, pH 7.0, at concentrations of 0.1% Emulgen A-60, Triton X-100, Triton X-405 or sodium cholate gamma proteobacterium Y-134

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.6 2-pyridylhydroxymethane sulfate 1 mM, 30 min at 4°C, 34% inhibition gamma proteobacterium Y-134
1.1.3.6 8-hydroxyquinoline 1 mM, 30 min at 4°C, 29% inhibition gamma proteobacterium Y-134
1.1.3.6 FAD 1 mM, 30 min at 4°C, 9% inhibition gamma proteobacterium Y-134
1.1.3.6 FMN 1 mM, 30 min at 4°C, 11% inhibition gamma proteobacterium Y-134
1.1.3.6 o-phenanthroline 1 mM, 30 min at 4°C, 30% inhibition gamma proteobacterium Y-134
1.1.3.6 potassium cyanide 1 mM, 30 min at 4°C, 12% inhibition gamma proteobacterium Y-134

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.3.6 MgCl2 1 mM, 30 min at 4°C, activation to 113% of control gamma proteobacterium Y-134
1.1.3.6 MnCl2 1 mM, 30 min at 4°C, 35% inhibition gamma proteobacterium Y-134

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.6 gamma proteobacterium Y-134
-
 uncultured
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.6
-
 gamma proteobacterium Y-134

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.6 additional information enzyme production reaches its maximum by incubation at 30°C for 12 d gamma proteobacterium Y-134
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.6 14.4
-
-
 gamma proteobacterium Y-134

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.6 7-dehydrocholesterol + O2 14% of the activity with cholesterol gamma proteobacterium Y-134 cholest-4,7-dien-3-one + H2O2
-
 ?
1.1.3.6 beta-sitosterol + O2 29% of the activity with cholesterol gamma proteobacterium Y-134 stigmast-5-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 gamma proteobacterium Y-134 cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 dihydrocholesterol + O2 79% of the activity with cholesterol gamma proteobacterium Y-134 5alpha-cholestan-3-one + H2O2
-
 ?
1.1.3.6 additional information no activity with: ergosterol, dehydroepiandrosterone, epiandrosterone, pregnenolone gamma proteobacterium Y-134 ?
-
 ?
1.1.3.6 stigmastanol + O2 15% of the activity with cholesterol gamma proteobacterium Y-134 stigmastanone + H2O2
-
 ?
1.1.3.6 stigmasterol + O2 32% of the activity with cholesterol gamma proteobacterium Y-134 stigmasta-5,22-dien-3-one + H2O2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.6 50
-
-
 gamma proteobacterium Y-134

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.3.6 30 70 30°C: about 85% of maximal activity, 70°C: about 80% of maximal activity gamma proteobacterium Y-134

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.6 60
-
 pH 7.0, 60 min, about 10% loss of activity gamma proteobacterium Y-134
1.1.3.6 70
-
 pH 7.0, 10 min, about 55% loss of activity gamma proteobacterium Y-134

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.6 6.5
-
-
 gamma proteobacterium Y-134

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.6 5 7.5 pH 5.0: about 70% of maximal activity, pH 7.5: about 30% of maximal activity gamma proteobacterium Y-134

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.6 5 6.5 60°C, 60 min, stable gamma proteobacterium Y-134
1.1.3.6 7.5
-
 60°C, 60 min, about 10% loss of activity gamma proteobacterium Y-134
1.1.3.6 8
-
 60°C, 60 min, about 40% loss of activity gamma proteobacterium Y-134

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.3.6 gamma proteobacterium Y-134 isoelectric focusing, pH-gradient 3.5-10.0
-
 4.2