EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.4 | AgNO3 | 1 mM, 30°C, complete loss of aminating activity | Klebsiella pneumoniae | |
1.4.1.4 | AlCl3 | 1 mM, 30°C, 23% loss of aminating activity | Klebsiella pneumoniae | |
1.4.1.4 | BaCl2 | 1 mM, 30°C, 15% loss of aminating activity | Klebsiella pneumoniae | |
1.4.1.4 | CaCl2 | 1 mM, 30°C, 11% loss of aminating activity | Klebsiella pneumoniae | |
1.4.1.4 | CH2ICOOH | 1 mM, 30°C, 15% loss of aminating activity | Klebsiella pneumoniae | |
1.4.1.4 | FeSO3(NH4)2SO4 | 1 mM, 30°C, 14% loss of aminating activity | Klebsiella pneumoniae | |
1.4.1.4 | HgCl2 | 1 mM, 30°C, 48% loss of aminating activity | Klebsiella pneumoniae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.1.4 | 52000 | - |
6 * 52000, SDS-PAGE | Klebsiella pneumoniae |
1.4.1.4 | 300000 | - |
gel filtration | Klebsiella pneumoniae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.4 | 2-oxoglutarate + NADPH + NH3 | Klebsiella pneumoniae | ammonia-assimilating enzyme | L-glutamate + NADP+ + H2O | - |
r | |
1.4.1.4 | 2-oxoglutarate + NADPH + NH3 | Klebsiella pneumoniae F-5-2 | ammonia-assimilating enzyme | L-glutamate + NADP+ + H2O | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.4 | Klebsiella pneumoniae | - |
- |
- |
1.4.1.4 | Klebsiella pneumoniae F-5-2 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.1.4 | - |
Klebsiella pneumoniae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.4.1.4 | 190 | - |
- |
Klebsiella pneumoniae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.4 | 2-oxoadipate + NADPH + NH3 | 6.3% of the activity with 2-oxoglutarate | Klebsiella pneumoniae | L-2-aminoadipate + NADP+ + H2O | - |
? | |
1.4.1.4 | 2-oxoadipate + NADPH + NH3 | 6.3% of the activity with 2-oxoglutarate | Klebsiella pneumoniae F-5-2 | L-2-aminoadipate + NADP+ + H2O | - |
? | |
1.4.1.4 | 2-oxobutyrate + NADPH + NH3 | 12% of the activity with 2-oxoglutarate | Klebsiella pneumoniae | L-2-aminobutyrate + NADP+ + H2O | - |
? | |
1.4.1.4 | 2-oxobutyrate + NADPH + NH3 | 12% of the activity with 2-oxoglutarate | Klebsiella pneumoniae F-5-2 | L-2-aminobutyrate + NADP+ + H2O | - |
? | |
1.4.1.4 | 2-oxoglutarate + NADPH + NH3 | - |
Klebsiella pneumoniae | L-glutamate + NADP+ + H2O | - |
r | |
1.4.1.4 | 2-oxoglutarate + NADPH + NH3 | ammonia-assimilating enzyme | Klebsiella pneumoniae | L-glutamate + NADP+ + H2O | - |
r | |
1.4.1.4 | 2-oxoglutarate + NADPH + NH3 | - |
Klebsiella pneumoniae F-5-2 | L-glutamate + NADP+ + H2O | - |
r | |
1.4.1.4 | 2-oxoglutarate + NADPH + NH3 | ammonia-assimilating enzyme | Klebsiella pneumoniae F-5-2 | L-glutamate + NADP+ + H2O | - |
r | |
1.4.1.4 | 2-oxovalerate + NADPH + NH3 | 15% of the activity with 2-oxoglutarate | Klebsiella pneumoniae | L-2-aminovalerate + NADP+ + H2O | - |
? | |
1.4.1.4 | additional information | no activity with 2-oxoisovalerate and pyruvate | Klebsiella pneumoniae | ? | - |
? | |
1.4.1.4 | additional information | no activity with 2-oxoisovalerate and pyruvate | Klebsiella pneumoniae F-5-2 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.4.1.4 | hexamer | 6 * 52000, SDS-PAGE | Klebsiella pneumoniae |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.1.4 | 50 | - |
pH 8.0, 10 min, maintains more than 90% activity | Klebsiella pneumoniae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.1.4 | 8 | - |
aminating activity | Klebsiella pneumoniae |
1.4.1.4 | 9 | 10 | deaminating activity | Klebsiella pneumoniae |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.4.1.4 | 5.5 | 11.5 | stable | Klebsiella pneumoniae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.4 | NADP+ | - |
Klebsiella pneumoniae | |
1.4.1.4 | NADPH | - |
Klebsiella pneumoniae |