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Literature summary extracted from

  • Muramatsu, H.; Mihara, H.; Kakutani, R.; Yasuda, M.; Ueda, M.; Kurihara, T.; Esaki, N.
    The putative malate/lactate dehydrogenase from Pseudomonas putida is an NADPH-dependent DELTA1-piperideine-2-carboxylate/DELTA1-pyrroline-2-carboxylate reductase involved in the catabolism of D-lysine and D-proline (2005), J. Biol. Chem., 280, 5329-5335.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.1
-
 Pseudomonas putida
1.5.1.21 DNA sequence determination and analysis, overexpression in Escherichia coli Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.5.1.1 additional information enzyme disruption mutant, strain is unable to use D-lysine or D-proline as a sole carbon source Pseudomonas putida
1.5.1.21 additional information dpk disruption mutant is unable to grow on D-lysine or D-proline as sole carbon source Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.21 NADPH inhibits the reverse reaction Pseudomonas putida
1.5.1.21 pyrroline-2-carboxylate inhibits the reverse reaction Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.21 additional information
-
 additional information kinetics Pseudomonas putida
1.5.1.21 0.034
-
 NADP+ pH 10.0, 35°C, with substrate L-proline Pseudomonas putida
1.5.1.21 0.034
-
 NADPH pH 8.0, 35°C, with substrate DELTA1-pyrroline-2-carboxylate Pseudomonas putida
1.5.1.21 0.14
-
 NADP+ pH 10.0, 35°C, with substrate L-pipecolate Pseudomonas putida
1.5.1.21 0.14
-
 NADPH pH 8.0, 35°C, with substrate DELTA1-piperideine-2-carboxylate Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.1 additional information Pseudomonas putida involved in the catabolism of D-lysine and D-proline ?
-
 ?
1.5.1.21 additional information Pseudomonas putida enzyme is involved in the catabolism of D-lysine and D-proline, pathway overview ?
-
 ?
1.5.1.21 additional information Pseudomonas putida ATCC 12633 enzyme is involved in the catabolism of D-lysine and D-proline, pathway overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.1 Pseudomonas putida
-
-
-
1.5.1.21 Pseudomonas putida Q5FB93 strain ATCC12633, gene dpkA
-
1.5.1.21 Pseudomonas putida ATCC 12633 Q5FB93 strain ATCC12633, gene dpkA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.21 recombinant enzyme from Escherichia coli, to homogeneity Pseudomonas putida

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.21 additional information specificity for carbon sources, wild-type shows the highest growth rate on D-proline, the deletion mutant on L-lysine, overview Pseudomonas putida
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.1 0.33
-
 reduction of DELTA1-pyrroline-2-carboxylate, cosubstrate NADPH, pH 8.0, 30°C Pseudomonas putida
1.5.1.1 0.67
-
 reduction of DELTA1-piperidine-2-carboxylate, cosubstrate NADH, pH 8.0, 30°C Pseudomonas putida
1.5.1.1 1.4
-
 oxidation of L-pipecolate, cosubstrate NADP+, pH 8.0, 30°C Pseudomonas putida
1.5.1.1 5.4
-
 oxidation of L-proline, cosubstrate NADP+, pH 8.0, 30°C Pseudomonas putida
1.5.1.1 51
-
 reduction of DELTA1-pyrroline-2-carboxylate, cosubstrate NADPH, pH 8.0, 30°C Pseudomonas putida
1.5.1.1 92
-
 reduction of DELTA1-piperidine-2-carboxylate, cosubstrate NADPH, pH 8.0, 30°C Pseudomonas putida
1.5.1.21 additional information
-
-
 Pseudomonas putida
1.5.1.21 0.33
-
 purified recombinant enzyme, substrates DELTA1-pyrroline-2-carboxylate and NADH Pseudomonas putida
1.5.1.21 0.67
-
 purified recombinant enzyme, substrates DELTA1-piperideine-2-carboxylate and NADH Pseudomonas putida
1.5.1.21 1.4
-
 purified recombinant enzyme, substrates L-pipecolate and NADP+ Pseudomonas putida
1.5.1.21 51
-
 purified recombinant enzyme, substrates DELTA1-pyrroline-2-carboxylate and NADPH Pseudomonas putida
1.5.1.21 92
-
 purified recombinant enzyme, substrates DELTA1-piperideine-2-carboxylate and NADPH Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.1 DELTA1-piperidine-2-carboxylate + NAD(P)H
-
 Pseudomonas putida L-pipecolic acid + NAD(P)+
-
 r
1.5.1.1 DELTA1-pyrroline-2-carboxylate + NAD(P)H at 55% of the rate with DELTA1-piperidine-2-carboxylate Pseudomonas putida L-proline + NAD(P)+
-
 r
1.5.1.1 L-pipecolic acid + NAD(P)+ at 26% of the rate with L-proline Pseudomonas putida DELTA1-piperidine-2-carboxylate + NAD(P)H
-
 r
1.5.1.1 L-proline + NAD(P)+
-
 Pseudomonas putida DELTA1-pyrroline-2-carboxylate + NAD(P)H + H+
-
 r
1.5.1.1 additional information involved in the catabolism of D-lysine and D-proline Pseudomonas putida ?
-
 ?
1.5.1.1 additional information reverse reactions oxidizing L-proline or L-pipecolic acid are catalyzed at much lower rates. Very poor substrates: trans-4-hydroxy-L-proline, L-thioproline Pseudomonas putida ?
-
 ?
1.5.1.21 DELTA1-piperideine-2-carboxylate + NADPH NADH is a poor electron donor, lower activity in the reverse reaction direction Pseudomonas putida L-pipecolate + NADP+
-
 r
1.5.1.21 DELTA1-piperideine-2-carboxylate + NADPH NADH is a poor electron donor, lower activity in the reverse reaction direction Pseudomonas putida ATCC 12633 L-pipecolate + NADP+
-
 r
1.5.1.21 DELTA1-pyrroline-2-carboxylate + NADPH NADH is a poor electron donor, lower activity in the reverse reaction direction Pseudomonas putida L-proline + NADP+
-
 r
1.5.1.21 DELTA1-pyrroline-2-carboxylate + NADPH NADH is a poor electron donor, lower activity in the reverse reaction direction Pseudomonas putida ATCC 12633 L-proline + NADP+
-
 r
1.5.1.21 additional information enzyme is involved in the catabolism of D-lysine and D-proline, pathway overview Pseudomonas putida ?
-
 ?
1.5.1.21 additional information substrate specificity, enzyme shows no malate or lactate dehydrogenase activity Pseudomonas putida ?
-
 ?
1.5.1.21 additional information enzyme is involved in the catabolism of D-lysine and D-proline, pathway overview Pseudomonas putida ATCC 12633 ?
-
 ?
1.5.1.21 additional information substrate specificity, enzyme shows no malate or lactate dehydrogenase activity Pseudomonas putida ATCC 12633 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.21 DELTA1-piperideine-2-carboxylate/DELTA1-pyrroline-2-carboxylate reductase
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.21 35
-
 assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.21 8
-
 assay at, forward reaction Pseudomonas putida
1.5.1.21 10
-
 assay at, reverse reaction Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.1 NAD+
-
 Pseudomonas putida
1.5.1.1 NADH reaction with NADH is less than 1% of that with NADPH Pseudomonas putida
1.5.1.1 NADP+
-
 Pseudomonas putida
1.5.1.1 NADPH reaction with NADH is less than 1% of that with NADPH Pseudomonas putida
1.5.1.21 NADPH dependent on Pseudomonas putida

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.21 additional information
-
 additional information substrate and product inhibition kinetics, overview Pseudomonas putida