Literature summary extracted from

Shi, W.; Mersfelder, J.; Hille, R.
The interaction of trimethylamine dehydrogenase and electron-transferring flavoprotein (2005), J. Biol. Chem., 280, 20239-20246.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.5.8.2	expression of wild-type and mutant T442G enzymes in Escherichia coli strain Bl21(DE3)	Methylophilus methylotrophus

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.5.8.2	Y442G	site-directed mutagenesis	Methylophilus methylotrophus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.5.8.2	additional information	-	additional information	kinetics, dissociation constants, thermodynamics	Methylophilus methylotrophus

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.5.8.2	Methylophilus methylotrophus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.5.8.2	native enzyme, and recombinant wild-type and mutant T442G enzymes expressed in Escherichia coli strain BL21(DE3)	Methylophilus methylotrophus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.5.8.2	trimethylamine + H2O + oxidized electron-transferring flavoprotein	-	Methylophilus methylotrophus	dimethylamine + formaldehyde + reduced electron-transferring flavoprotein	-	r
1.5.8.2	trimethylamine + H2O + semiquinone electron-transferring flavoprotein	-	Methylophilus methylotrophus	dimethylamine + formaldehyde + reduced electron-transferring flavoprotein	-	r

Synonyms

EC Number	Synonyms	Comment	Organism
1.5.8.2	TMADH	-	Methylophilus methylotrophus

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.5.8.2	additional information	-	Abz-VAA	-	Methylophilus methylotrophus

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.5.8.2	electron-transferring flavoprotein	interaction with the enzyme, binding study, reaction mechanism and kinetics	Methylophilus methylotrophus
1.5.8.2	FAD	-	Methylophilus methylotrophus

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Release 2023.1 (January 2023)