BRENDA - Enzyme Database

General Enzymatic Screens Identify Three New Nucleotidases in Escherichia coli. Biochemical Characterization of SurE, YfbR, and Yjjg

Proudfoot, M.; Kuznetsova, E.; Brown, G.; Rao, N.N.; Kitagawa, M.; Mori, H.; Savchenko, A.; Yakunin, A.F.; J. Biol. Chem. 279, 54687-54694 (2004)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.3.5
-
Escherichia coli
3.1.3.6
-
Escherichia coli
3.6.1.11
-
Escherichia coli
General Stability
EC Number
General Stability
Organism
3.1.3.5
stable
Escherichia coli
3.1.3.6
stable
Escherichia coli
3.6.1.11
stable
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.5
ADP
;
Escherichia coli
3.1.3.5
ATP
;
Escherichia coli
3.1.3.5
CTP
-
Escherichia coli
3.1.3.5
dATP
;
Escherichia coli
3.1.3.5
dCTP
-
Escherichia coli
3.1.3.5
dGTP
-
Escherichia coli
3.1.3.5
dTTP
-
Escherichia coli
3.1.3.5
GTP
-
Escherichia coli
3.1.3.5
ITP
-
Escherichia coli
3.1.3.5
TTP
-
Escherichia coli
3.1.3.5
UTP
-
Escherichia coli
3.1.3.6
ATP
-
Escherichia coli
3.1.3.6
CTP
-
Escherichia coli
3.1.3.6
dATP
-
Escherichia coli
3.1.3.6
dCTP
-
Escherichia coli
3.1.3.6
dGTP
-
Escherichia coli
3.1.3.6
dTTP
-
Escherichia coli
3.1.3.6
GTP
-
Escherichia coli
3.1.3.6
ITP
-
Escherichia coli
3.1.3.6
TTP
-
Escherichia coli
3.1.3.6
UTP
-
Escherichia coli
3.1.3.31
ADP
inhibits SurE slightly, YfbR, not YjjG, competitive
Escherichia coli
3.1.3.31
ATP
inhibits SurE, YfbR, not YjjG, competitive
Escherichia coli
3.1.3.31
CTP
inhibits SurE, YfbR, not YjjG, competitive
Escherichia coli
3.1.3.31
dATP
inhibits SurE, YfbR, not YjjG, competitive
Escherichia coli
3.1.3.31
dCTP
inhibits SurE, YfbR, not YjjG, competitive
Escherichia coli
3.1.3.31
dGTP
inhibits SurE, YfbR, not YjjG, competitive
Escherichia coli
3.1.3.31
dTTP
inhibits SurE, YfbR, not YjjG, competitive
Escherichia coli
3.1.3.31
GTP
inhibits SurE, YfbR, not YjjG, competitive
Escherichia coli
3.1.3.31
ITP
inhibits SurE, YfbR, not YjjG, competitive
Escherichia coli
3.1.3.31
additional information
inhibitory potency of nucleotides, overview, AMP is a poor inhibitor for SurE, YfbR, and YjjG, YjjG is not inhibitable by nucleotides
Escherichia coli
3.1.3.31
TTP
inhibits SurE, YfbR, not YjjG, competitive
Escherichia coli
3.1.3.31
UTP
inhibits SurE, YfbR, and slightly YjjG, competitive
Escherichia coli
3.1.3.89
ADP
0.2 mM, 21% residual activity
Escherichia coli
3.1.3.89
ATP
0.2 mM, 18% residual activity
Escherichia coli
3.1.3.89
CTP
0.2 mM, 29% residual activity
Escherichia coli
3.1.3.89
dATP
0.2 mM, 15% residual activity
Escherichia coli
3.1.3.89
dCTP
0.2 mM, 19% residual activity
Escherichia coli
3.1.3.89
dGTP
0.2 mM, 13% residual activity
Escherichia coli
3.1.3.89
dTTP
0.2 mM, 15% residual activity
Escherichia coli
3.1.3.89
GTP
0.2 mM, 28% residual activity
Escherichia coli
3.1.3.89
ITP
0.2 mM, 29% residual activity
Escherichia coli
3.1.3.89
TTP
0.2 mM, 14% residual activity
Escherichia coli
3.1.3.89
UTP
0.2 mM, 26% residual activity
Escherichia coli
3.6.1.11
ATP
-
Escherichia coli
3.6.1.11
CTP
-
Escherichia coli
3.6.1.11
dATP
-
Escherichia coli
3.6.1.11
dCTP
-
Escherichia coli
3.6.1.11
dGTP
-
Escherichia coli
3.6.1.11
dTTP
-
Escherichia coli
3.6.1.11
GTP
-
Escherichia coli
3.6.1.11
ITP
-
Escherichia coli
3.6.1.11
TTP
-
Escherichia coli
3.6.1.11
UTP
-
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.5
0.008
-
5'-dTMP
+/- 0.0003
Escherichia coli
3.1.3.5
0.012
-
5'-dAMP
+/- 0.001
Escherichia coli
3.1.3.5
0.017
-
5'-dIMP
+/- 0.001
Escherichia coli
3.1.3.5
0.02
-
5'-dUMP
+/- 0.001
Escherichia coli
3.1.3.5
0.02
-
Polyphosphate
+/- 0.003
Escherichia coli
3.1.3.5
0.036
-
5'-dCMP
+/- 0.002
Escherichia coli
3.1.3.5
0.047
-
5'-dGMP
+/- 0.005
Escherichia coli
3.1.3.5
0.1
-
3'-AMP
+/- 0.01
Escherichia coli
3.1.3.5
0.26
-
5'-GMP
+/- 0.05
Escherichia coli
3.1.3.5
0.28
-
5'-dGMP
+/- 0.04
Escherichia coli
3.1.3.5
0.32
-
5'-AMP
+/- 0.04
Escherichia coli
3.1.3.5
0.37
-
3'-CMP
+/- 0.08
Escherichia coli
3.1.3.5
2.09
-
p-nitrophenyl phosphate
+/- 0.13
Escherichia coli
3.1.3.5
2.49
-
p-nitrophenyl phosphate
+/- 0.4
Escherichia coli
3.1.3.6
0.02
-
Polyphosphate
+/- 0.003
Escherichia coli
3.1.3.6
0.1
-
3'-AMP
+/- 0.01
Escherichia coli
3.1.3.6
0.26
-
5'-GMP
+/- 0.05
Escherichia coli
3.1.3.6
0.28
-
5'-dGMP
+/- 0.04
Escherichia coli
3.1.3.6
0.32
-
5'-AMP
+/- 0.04
Escherichia coli
3.1.3.6
0.37
-
3'-CMP
+/- 0.08
Escherichia coli
3.1.3.6
2.49
-
p-nitrophenyl phosphate
+/- 0.4
Escherichia coli
3.1.3.31
0.008
-
5'-dTMP
pH 8.0, 37°C, YfbR, with Co2+
Escherichia coli
3.1.3.31
0.012
-
5'-dAMP
pH 8.0, 37°C, YfbR, with Co2+
Escherichia coli
3.1.3.31
0.017
-
5'-dIMP
pH 8.0, 37°C, YfbR, with Co2+
Escherichia coli
3.1.3.31
0.02
-
5'-dUMP
pH 8.0, 37°C, YfbR, with Co2+
Escherichia coli
3.1.3.31
0.02
-
Polyphosphate
pH 7.0, 37°C, SurE, with Mg2+
Escherichia coli
3.1.3.31
0.036
-
5'-dCMP
pH 8.0, 37°C, YfbR, with Co2+
Escherichia coli
3.1.3.31
0.047
-
5'-dGMP
pH 8.0, 37°C, YfbR, with Co2+
Escherichia coli
3.1.3.31
0.1
-
3'-AMP
pH 7.0, 37°C, SurE, with Mn2+
Escherichia coli
3.1.3.31
0.26
-
5'-GMP
pH 7.0, 37°C, SurE, with Mn2+
Escherichia coli
3.1.3.31
0.28
-
5'-dGMP
pH 7.0, 37°C, SurE, with Mn2+
Escherichia coli
3.1.3.31
0.32
-
5'-AMP
pH 7.0, 37°C, SurE, with Mn2+
Escherichia coli
3.1.3.31
0.37
-
3'-CMP
pH 7.0, 37°C, SurE, with Mn2+
Escherichia coli
3.1.3.31
0.51
-
5'-dTMP
pH 7.5, 37°C, YjjG, with Mn2+
Escherichia coli
3.1.3.31
0.66
-
5'-UMP
pH 7.5, 37°C, YjjG, with Mn2+
Escherichia coli
3.1.3.31
0.77
-
5'-dUMP
pH 7.5, 37°C, YjjG, with Mn2+
Escherichia coli
3.1.3.31
2.09
-
4-nitrophenyl phosphate
pH 8.0, 37°C, YfbR, with Co2+
Escherichia coli
3.1.3.31
2.49
-
4-nitrophenyl phosphate
pH 7.0, 37°C, SurE, with Mn2+
Escherichia coli
3.1.3.31
17.8
-
4-nitrophenyl phosphate
pH 7.5, 37°C, YjjG, with Mn2+
Escherichia coli
3.1.3.89
0.008
-
5'-dTMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
0.012
-
5'-dAMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
0.017
-
5'-dIMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
0.02
-
5'-dUMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
0.036
-
5'-dCMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
0.047
-
5'-dGMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
2.09
-
4-nitrophenyl phosphate
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.6.1.11
0.02
-
Polyphosphate
+/- 0.003
Escherichia coli
3.6.1.11
0.1
-
3'-AMP
+/- 0.01
Escherichia coli
3.6.1.11
0.26
-
5'-GMP
+/- 0.05
Escherichia coli
3.6.1.11
0.28
-
5'-dGMP
+/- 0.04
Escherichia coli
3.6.1.11
0.32
-
5'-AMP
+/- 0.04
Escherichia coli
3.6.1.11
0.37
-
3'-CMP
+/- 0.08
Escherichia coli
3.6.1.11
2.49
-
p-nitrophenyl phosphate
+/- 0.4
Escherichia coli
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.5
Co2+
activates with 5'-dAMP, KD: 67.6 +/- 2.86 micorM; activates with p-nitrophenyl phosphate, KD: 537.7 +/- 19.7 microM; KD: 46.9 +/- 5.66 microM Co2+ with p-nitrophenyl phosphate, KD: 10.7 +/- 1.07 microM Co2+ with 5'-AMP
Escherichia coli
3.1.3.5
Mg2+
KD: 224.7 +/- 35.1 microM Mg2+ with p-nitrophenyl phosphate, KD: 140.0 +/- 9.99 microM Mg2+ with 5'-AMP
Escherichia coli
3.1.3.5
Mn2+
KD: 7.24 +/- 0.71 microM Mn2+ with p-nitrophenyl phosphate, KD: 2.23 +/- 0.14 micorM Mn2+ with 5'-AMP
Escherichia coli
3.1.3.5
Ni2+
KD: 72.3 +/- 7.61 microM Ni2+ with p-nitrophenyl phosphate, KD: 29.4 +/- 3.69 microM Ni2+ with 5'-AMP
Escherichia coli
3.1.3.6
Co2+
KD: 46.9 +/- 5.66 microM Co2+ with p-nitrophenyl phosphate, KD: 10.7 +/- 1.07 microM Co2+ with 5'-AMP
Escherichia coli
3.1.3.6
Mg2+
KD: 224.7 +/- 35.1 microM Mg2+ with p-nitrophenyl phosphate, KD: 140.0 +/- 9.99 microM Mg2+ with 5'-AMP
Escherichia coli
3.1.3.6
Mn2+
KD: 7.24 +/- 0.71 microM Mn2+ with p-nitrophenyl phosphate, KD: 2.23 +/- 0.14 micorM Mn2+ with 5'-AMP
Escherichia coli
3.1.3.6
Ni2+
KD: 72.3 +/- 7.61 microM Ni2+ with p-nitrophenyl phosphate, KD: 29.4 +/- 3.69 microM Ni2+ with 5'-AMP
Escherichia coli
3.1.3.31
Co2+
preferred by YfbR, activates SurE, YfbR, and YjjG
Escherichia coli
3.1.3.31
Cu2+
activates YfbR
Escherichia coli
3.1.3.31
Mg2+
preferred by YjjG, activates SurE and YjjG
Escherichia coli
3.1.3.31
Mn2+
preferred by SurE, activates SurE, YfbR, and YjjG
Escherichia coli
3.1.3.31
additional information
dependent on divalent cations: SurE prefers Mn2+ to Co2+, Ni2+, and Mg2+, YfbR prefers Co2+ to Mn2+ and Cu2+, YjjG prefers Mg2+ to Mn2+ and Co2+, in descending orders, overview
Escherichia coli
3.1.3.31
Ni2+
activates SurE
Escherichia coli
3.1.3.89
Co2+
presence of divalent cation absolutely required. Maximum activity with Co2+, followed in decreasing order by Mn2+, Cu2+. Apparent Kd value for Co2+ 0.8 mM
Escherichia coli
3.1.3.89
Cu2+
presence of divalent cation absolutely required. Maximum activity with Co2+, followed in decreasing order by Mn2+, Cu2+
Escherichia coli
3.1.3.89
Mn2+
presence of divalent cation absolutely required. Maximum activity with Co2+, followed in decreasing order by Mn2+, Cu2+
Escherichia coli
3.6.1.11
Co2+
KD: 46.9 +/- 5.66 micorM Co2+ with p-nitrophenyl phosphate, KD: 10.7 +/- 1.07 microM Co2+ with 5'-AMP
Escherichia coli
3.6.1.11
Mg2+
KD: 224.7 +/- 35.1 microM Mg2+ with p-nitrophenyl phosphate, KD: 140.0 +/- 9.99 microM Mg2+ with 5'-AMP
Escherichia coli
3.6.1.11
Mn2+
KD: 7.24 +/- 0.71 microM Mn2+ with p-nitrophenyl phosphate, KD: 2.23 +/- 0.14 micorM Mn2+ with 5'-AMP
Escherichia coli
3.6.1.11
Ni2+
KD: 72.3 +/- 7.61 microM Ni2+ with p-nitrophenyl phosphate, KD: 29.4 +/- 3.69 microM Ni2+ with 5'-AMP
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.31
35700
-
monomeric YjjG, gel filtration
Escherichia coli
3.1.3.31
41600
-
monomeric SurE, gel filtration
Escherichia coli
3.1.3.31
52800
-
dimeric YjjG, gel filtration
Escherichia coli
3.1.3.31
71300
-
trimeric YfbR, gel filtration
Escherichia coli
3.1.3.31
100000
-
above, tetrameric YjjG and YfbR, gel filtration
Escherichia coli
3.1.3.89
24000
-
and tetramer, 3 * 24000, SDS-PAGE; and trimer, 4 * 24000, SDS-PAGE
Escherichia coli
3.1.3.89
71300
-
and above 100000, gel filtration
Escherichia coli
3.1.3.89
100000
-
and 71300, gel filtration
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.5
Escherichia coli
P0A840
-
-
3.1.3.5
Escherichia coli
P76491
-
-
3.1.3.6
Escherichia coli
P0A840
-
-
3.1.3.31
Escherichia coli
-
genes surE, yfbR, and yjjG
-
3.1.3.89
Escherichia coli
-
-
-
3.6.1.11
Escherichia coli
P0A840
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.5
0.1
-
+/- 0.01, polyphosphate
Escherichia coli
3.1.3.5
0.37
-
+/- 0.01, 5'-dTMP
Escherichia coli
3.1.3.5
0.46
-
+/- 0.03, 5'-dGMP
Escherichia coli
3.1.3.5
0.52
-
+/- 0.02, 5'-dCMP
Escherichia coli
3.1.3.5
0.54
-
+/- 0.02, 5'-dUMP
Escherichia coli
3.1.3.5
0.56
-
+/- 0.01, 5'-dIMP
Escherichia coli
3.1.3.5
0.71
-
+/- 0.02, 5'-dAMP
Escherichia coli
3.1.3.5
1.32
-
+/- 0.02, p-nitrophenyl phosphate
Escherichia coli
3.1.3.5
7.24
-
+/- 0.34, p-nitrophenyl phosphate
Escherichia coli
3.1.3.5
10
-
+/- 0.61, 5'-AMP
Escherichia coli
3.1.3.5
12.1
-
+/- 0.90, 3'-CMP
Escherichia coli
3.1.3.5
16.4
-
+/- 0.80, 5'-dGMP
Escherichia coli
3.1.3.5
20.1
-
+/- 0.76, 3'-AMP
Escherichia coli
3.1.3.5
22.4
-
+/- 1.78, 5'-GMP
Escherichia coli
3.1.3.6
0.1
-
+/- 0.01, polyphosphate
Escherichia coli
3.1.3.6
7.24
-
+/- 0.34, p-nitrophenyl phosphate
Escherichia coli
3.1.3.6
10
-
+/- 0.61, 5'-AMP
Escherichia coli
3.1.3.6
12.1
-
+/- 0.90, 3'-CMP
Escherichia coli
3.1.3.6
16.4
-
+/- 0.80, 5'-dGMP
Escherichia coli
3.1.3.6
20.1
-
+/- 0.76, 3'-AMP
Escherichia coli
3.1.3.6
22.4
-
+/- 1.78, 5'-GMP
Escherichia coli
3.1.3.31
additional information
-
-
Escherichia coli
3.6.1.11
0.1
-
+/- 0.01, polyphosphate
Escherichia coli
3.6.1.11
7.24
-
+/- 0.34, p-nitrophenyl phosphate
Escherichia coli
3.6.1.11
10
-
+/- 0.61, 5'-AMP
Escherichia coli
3.6.1.11
12.1
-
+/- 0.90, 3'-CMP
Escherichia coli
3.6.1.11
16.4
-
+/- 0.80, 5'-dGMP
Escherichia coli
3.6.1.11
20.1
-
+/- 0.76, 3'-AMP
Escherichia coli
3.6.1.11
22.4
-
+/- 1.78, 5'-GMP
Escherichia coli
Storage Stability
EC Number
Storage Stability
Organism
3.1.3.5
4°C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months
Escherichia coli
3.1.3.6
4°C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months
Escherichia coli
3.6.1.11
4°C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.5
(polyphosphate)n + H2O
-
656266
Escherichia coli
(polyphosphate)n-1 + phosphate
-
-
-
-
3.1.3.5
3'-AMP + H2O
-
656266
Escherichia coli
adenosine + phosphate
-
-
-
-
3.1.3.5
3'-CMP + H2O
-
656266
Escherichia coli
cytosine + phosphate
-
-
-
-
3.1.3.5
5'-AMP + H2O
-
656266
Escherichia coli
adenosine + phosphate
-
-
-
-
3.1.3.5
5'-dAMP + H2O
-
656266
Escherichia coli
deoxyadenosine + phosphate
-
-
-
-
3.1.3.5
5'-dCMP + H2O
-
656266
Escherichia coli
2'-deoxycytidine + phosphate
-
-
-
-
3.1.3.5
5'-dGMP + H2O
-
656266
Escherichia coli
deoxyguanosine + phosphate
-
-
-
-
3.1.3.5
5'-dIMP + H2O
-
656266
Escherichia coli
deoxyinosine + phosphate
-
-
-
-
3.1.3.5
5'-dTMP + H2O
-
656266
Escherichia coli
deoxythymidine + phosphate
-
-
-
-
3.1.3.5
5'-dUMP + H2O
-
656266
Escherichia coli
deoxyuridine + phosphate
-
-
-
-
3.1.3.5
5'-GMP + H2O
-
656266
Escherichia coli
guanosine + phosphate
-
-
-
-
3.1.3.5
p-nitrophenyl phosphate + H2O
-
656266
Escherichia coli
p-nitrophenol + phosphate
-
-
-
-
3.1.3.6
(polyphosphate)n + H2O
-
656266
Escherichia coli
(polyphosphate)(n-1) + phosphate
-
-
-
-
3.1.3.6
3'-AMP + H2O
-
656266
Escherichia coli
adenosine + phosphate
-
-
-
-
3.1.3.6
3'-CMP + H2O
-
656266
Escherichia coli
cytosine + phosphate
-
-
-
-
3.1.3.6
5'-AMP + H2O
-
656266
Escherichia coli
adenosine + phosphate
-
-
-
-
3.1.3.6
5'-dGMP + H2O
-
656266
Escherichia coli
deoxyguanosine + phosphate
-
-
-
-
3.1.3.6
5'-GMP + H2O
-
656266
Escherichia coli
guanosine + phosphate
-
-
-
-
3.1.3.6
p-nitrophenyl phosphate + H2O
-
656266
Escherichia coli
p-nitrophenol + phosphate
-
-
-
-
3.1.3.31
3'-AMP + H2O
-
656266
Escherichia coli
adenosine + phosphate
-
-
-
?
3.1.3.31
3'-CMP + H2O
-
656266
Escherichia coli
cytosine + phosphate
-
-
-
?
3.1.3.31
4-nitrophenyl phosphate + H2O
-
656266
Escherichia coli
4-nitrophenol + phosphate
-
-
-
?
3.1.3.31
5'-AMP + H2O
-
656266
Escherichia coli
adenosine + phosphate
-
-
-
?
3.1.3.31
5'-CMP + H2O
-
656266
Escherichia coli
cytidine + phosphate
-
-
-
?
3.1.3.31
5'-dAMP + H2O
-
656266
Escherichia coli
deoxyadenosine + phosphate
-
-
-
?
3.1.3.31
5'-dCMP + H2O
-
656266
Escherichia coli
2'-deoxycytidine + phosphate
-
-
-
?
3.1.3.31
5'-dGMP + H2O
-
656266
Escherichia coli
deoxyguanosine + phosphate
-
-
-
?
3.1.3.31
5'-dIMP + H2O
YfbR
656266
Escherichia coli
hypoxanthine + phosphate
-
-
-
?
3.1.3.31
5'-dTMP + H2O
-
656266
Escherichia coli
deoxythymidine + phosphate
-
-
-
?
3.1.3.31
5'-dUMP + H2O
-
656266
Escherichia coli
deoxyuridine + phosphate
-
-
-
-
3.1.3.31
5'-GMP + H2O
-
656266
Escherichia coli
guanosine + phosphate
-
-
-
?
3.1.3.31
5'-TMP + H2O
-
656266
Escherichia coli
thymidine + phosphate
-
-
-
?
3.1.3.31
additional information
substrate specificities of SurE, YfbR, and YjjG, overview
656266
Escherichia coli
?
-
-
-
-
3.1.3.31
polyphosphate + H2O
SurE
656266
Escherichia coli
phosphate
-
-
-
?
3.1.3.89
4-nitrophenyl phosphate + H2O
-
656266
Escherichia coli
4-nitrophenol + phosphate
-
-
-
?
3.1.3.89
5'-dAMP + H2O
-
656266
Escherichia coli
deoxyadenosine + phosphate
-
-
-
?
3.1.3.89
5'-dCMP + H2O
-
656266
Escherichia coli
deoxycytidine + phosphate
-
-
-
?
3.1.3.89
5'-dGMP + H2O
-
656266
Escherichia coli
deoxyguanosine + phosphate
-
-
-
?
3.1.3.89
5'-dIMP + H2O
-
656266
Escherichia coli
deoxyinosine + phosphate
-
-
-
?
3.1.3.89
5'-dTMP + H2O
-
656266
Escherichia coli
thymidine + phosphate
-
-
-
?
3.1.3.89
5'-dUMP + H2O
-
656266
Escherichia coli
deoxyuridine + phosphate
-
-
-
?
3.1.3.89
additional information
enzyme is strictly specific to deoxyribonucleoside 5'-monophosphates and does not hydrolyze ribonucleotides or deoxyribonucleoside 3'-monophosphates. It has similar levels of activity against all tested deoxyribonucleoside 5'-monophosphates
656266
Escherichia coli
?
-
-
-
-
3.6.1.11
(polyphosphate)n + H2O
-
656266
Escherichia coli
(polyphosphate)n-1 + phosphate
-
-
-
-
3.6.1.11
3'-AMP + H2O
-
656266
Escherichia coli
adenosine + phosphate
-
-
-
-
3.6.1.11
3'-CMP + H2O
-
656266
Escherichia coli
cytosine + phosphate
-
-
-
-
3.6.1.11
5'-AMP + H2O
-
656266
Escherichia coli
adenosine + phosphate
-
-
-
-
3.6.1.11
5'-dGMP + H2O
-
656266
Escherichia coli
deoxyguanosine + phosphate
-
-
-
-
3.6.1.11
5'-GMP + H2O
-
656266
Escherichia coli
guanosine + phosphate
-
-
-
-
3.6.1.11
p-nitrophenyl phosphate + H2O
-
656266
Escherichia coli
p-nitrophenol + phosphate
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
3.1.3.5
monomer
in solution
Escherichia coli
3.1.3.5
oligomer
at least four identical subunits in solution; three or more subunits in solution
Escherichia coli
3.1.3.6
monomer
in solution
Escherichia coli
3.1.3.6
oligomer
at least four identical subunits in solution
Escherichia coli
3.1.3.31
More
SurE, YfbR, and YjjG show monomeric, dimeric, trimeric, and tetrameric forms, overview
Escherichia coli
3.1.3.31
oligomer
x * 35700, YjjG, SDS-PAGE and gel filtration, x * 41600, SurE, SDS-PAGE and gel filtration
Escherichia coli
3.1.3.89
tetramer
and trimer, 4 * 24000, SDS-PAGE
Escherichia coli
3.1.3.89
trimer
and tetramer, 3 * 24000, SDS-PAGE
Escherichia coli
3.6.1.11
monomer
in solution
Escherichia coli
3.6.1.11
oligomer
at least four subunits in solution
Escherichia coli
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.3.31
37
-
assay at
Escherichia coli
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.1.3.5
0.05
-
Polyphosphate
+/- 0.005
Escherichia coli
3.1.3.5
0.16
-
5'-dTMP
+/- 0.01
Escherichia coli
3.1.3.5
0.2
-
5'-dGMP
+/- 0.01
Escherichia coli
3.1.3.5
0.23
-
5'-dCMP
+/- 0.01
Escherichia coli
3.1.3.5
0.23
-
5'-dUMP
+/- 0.01
Escherichia coli
3.1.3.5
0.24
-
5'-dIMP
+/- 0.01
Escherichia coli
3.1.3.5
0.3
-
5'-dAMP
+/- 0.01
Escherichia coli
3.1.3.5
0.56
-
p-nitrophenyl phosphate
+/- 0.01
Escherichia coli
3.1.3.5
3.55
-
p-nitrophenyl phosphate
+/- 0.16
Escherichia coli
3.1.3.5
4.9
-
5'-AMP
+/- 0.3
Escherichia coli
3.1.3.5
5.93
-
3'-CMP
+/- 0.44
Escherichia coli
3.1.3.5
8.04
-
5'-dGMP
+/- 0.39
Escherichia coli
3.1.3.5
9.84
-
3'-AMP
+/- 0.37
Escherichia coli
3.1.3.5
11
-
5'-GMP
+/- 0.87
Escherichia coli
3.1.3.6
0.05
-
Polyphosphate
+/- 0.005
Escherichia coli
3.1.3.6
3.55
-
p-nitrophenyl phosphate
+/- 0.16
Escherichia coli
3.1.3.6
4.9
-
5'-AMP
+/- 0.3
Escherichia coli
3.1.3.6
5.93
-
3'-CMP
+/- 0.44
Escherichia coli
3.1.3.6
8.04
-
5'-dGMP
+/- 0.39
Escherichia coli
3.1.3.6
9.84
-
3'-AMP
+/- 0.37
Escherichia coli
3.1.3.6
11
-
5'-GMP
+/- 0.87
Escherichia coli
3.1.3.31
0.05
-
Polyphosphate
pH 7.0, 37°C, SurE, with Mg2+
Escherichia coli
3.1.3.31
0.16
-
5'-dTMP
pH 8.0, 37°C, YfbR, with Co2+
Escherichia coli
3.1.3.31
0.2
-
5'-dGMP
pH 8.0, 37°C, YfbR, with Co2+
Escherichia coli
3.1.3.31
0.23
-
5'-dCMP
pH 8.0, 37°C, YfbR, with Co2+
Escherichia coli
3.1.3.31
0.23
-
5'-dUMP
pH 8.0, 37°C, YfbR, with Co2+
Escherichia coli
3.1.3.31
0.24
-
5'-dIMP
pH 8.0, 37°C, YfbR, with Co2+
Escherichia coli
3.1.3.31
0.3
-
5'-dAMP
pH 8.0, 37°C, YfbR, with Co2+
Escherichia coli
3.1.3.31
0.56
-
4-nitrophenyl phosphate
pH 8.0, 37°C, YfbR, with Co2+
Escherichia coli
3.1.3.31
3.55
-
4-nitrophenyl phosphate
pH 7.0, 37°C, SurE, with Mn2+
Escherichia coli
3.1.3.31
4.1
-
4-nitrophenyl phosphate
pH 7.5, 37°C, YjjG, with Mn2+
Escherichia coli
3.1.3.31
4.9
-
5'-AMP
pH 7.0, 37°C, SurE, with Mn2+
Escherichia coli
3.1.3.31
5.93
-
3'-CMP
pH 7.0, 37°C, SurE, with Mn2+
Escherichia coli
3.1.3.31
8.04
-
5'-dGMP
pH 7.0, 37°C, SurE, with Mn2+
Escherichia coli
3.1.3.31
9.84
-
3'-AMP
pH 7.0, 37°C, SurE, with Mn2+
Escherichia coli
3.1.3.31
11
-
5'-GMP
pH 7.0, 37°C, SurE, with Mn2+
Escherichia coli
3.1.3.31
21.4
-
5'-dUMP
pH 7.5, 37°C, YjjG, with Mn2+
Escherichia coli
3.1.3.31
30.4
-
5'-dTMP
pH 7.5, 37°C, YjjG, with Mn2+
Escherichia coli
3.1.3.31
34.2
-
5'-UMP
pH 7.5, 37°C, YjjG, with Mn2+
Escherichia coli
3.1.3.89
0.16
-
5'-dTMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
0.2
-
5'-dGMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
0.23
-
5'-dCMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
0.23
-
5'-dUMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
0.24
-
5'-dIMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
0.3
-
5'-dAMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
0.56
-
4-nitrophenyl phosphate
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.6.1.11
0.05
-
Polyphosphate
+/- 0.005
Escherichia coli
3.6.1.11
3.55
-
p-nitrophenyl phosphate
+/- 0.16
Escherichia coli
3.6.1.11
4.9
-
5'-AMP
+/- 0.3
Escherichia coli
3.6.1.11
5.93
-
3'-CMP
+/- 0.44
Escherichia coli
3.6.1.11
8.04
-
5'-dGMP
+/- 0.39
Escherichia coli
3.6.1.11
9.84
-
3'-AMP
+/- 0.37
Escherichia coli
3.6.1.11
11
-
5'-GMP
+/- 0.87
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.5
7
-
-
Escherichia coli
3.1.3.5
8
-
-
Escherichia coli
3.1.3.6
7
-
-
Escherichia coli
3.1.3.31
7
-
SurE
Escherichia coli
3.1.3.31
7.5
-
YjjG
Escherichia coli
3.1.3.31
8
-
YfbR
Escherichia coli
3.6.1.11
7
-
-
Escherichia coli
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.3.89
7
8
-
Escherichia coli
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.1.3.5
1.3
-
ADP
-
Escherichia coli
3.1.3.5
1.39
-
dATP
-
Escherichia coli
3.1.3.5
1.65
-
ATP
-
Escherichia coli
3.1.3.5
24.1
-
ATP
with 3'-AMP as substrate
Escherichia coli
3.1.3.6
24.1
-
ATP
with 3'-AMP as substrate
Escherichia coli
3.1.3.31
0.0013
-
ADP
pH 8.0, 37°C, YfbR, versus 5'-dAMP, with Co2+
Escherichia coli
3.1.3.31
0.0014
-
dATP
pH 8.0, 37°C, YfbR, versus 5'-dAMP, with Co2+
Escherichia coli
3.1.3.31
0.0017
-
ATP
pH 8.0, 37°C, YfbR, versus 5'-dAMP, with Co2+
Escherichia coli
3.1.3.31
0.024
-
ATP
pH 7.0, 37°C, SurE, versus 3'-AMP, with Mn2+
Escherichia coli
3.6.1.11
24.1
-
ATP
with 3'-AMP as substrate
Escherichia coli
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.5
-
Escherichia coli
3.1.3.6
-
Escherichia coli
3.6.1.11
-
Escherichia coli
General Stability (protein specific)
EC Number
General Stability
Organism
3.1.3.5
stable
Escherichia coli
3.1.3.6
stable
Escherichia coli
3.6.1.11
stable
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.5
ADP
-
Escherichia coli
3.1.3.5
ATP
-
Escherichia coli
3.1.3.5
CTP
-
Escherichia coli
3.1.3.5
dATP
-
Escherichia coli
3.1.3.5
dCTP
-
Escherichia coli
3.1.3.5
dGTP
-
Escherichia coli
3.1.3.5
dTTP
-
Escherichia coli
3.1.3.5
GTP
-
Escherichia coli
3.1.3.5
ITP
-
Escherichia coli
3.1.3.5
TTP
-
Escherichia coli
3.1.3.5
UTP
-
Escherichia coli
3.1.3.6
ATP
-
Escherichia coli
3.1.3.6
CTP
-
Escherichia coli
3.1.3.6
dATP
-
Escherichia coli
3.1.3.6
dCTP
-
Escherichia coli
3.1.3.6
dGTP
-
Escherichia coli
3.1.3.6
dTTP
-
Escherichia coli
3.1.3.6
GTP
-
Escherichia coli
3.1.3.6
ITP
-
Escherichia coli
3.1.3.6
TTP
-
Escherichia coli
3.1.3.6
UTP
-
Escherichia coli
3.1.3.31
ADP
inhibits SurE slightly, YfbR, not YjjG, competitive
Escherichia coli
3.1.3.31
ATP
inhibits SurE, YfbR, not YjjG, competitive
Escherichia coli
3.1.3.31
CTP
inhibits SurE, YfbR, not YjjG, competitive
Escherichia coli
3.1.3.31
dATP
inhibits SurE, YfbR, not YjjG, competitive
Escherichia coli
3.1.3.31
dCTP
inhibits SurE, YfbR, not YjjG, competitive
Escherichia coli
3.1.3.31
dGTP
inhibits SurE, YfbR, not YjjG, competitive
Escherichia coli
3.1.3.31
dTTP
inhibits SurE, YfbR, not YjjG, competitive
Escherichia coli
3.1.3.31
GTP
inhibits SurE, YfbR, not YjjG, competitive
Escherichia coli
3.1.3.31
ITP
inhibits SurE, YfbR, not YjjG, competitive
Escherichia coli
3.1.3.31
additional information
inhibitory potency of nucleotides, overview, AMP is a poor inhibitor for SurE, YfbR, and YjjG, YjjG is not inhibitable by nucleotides
Escherichia coli
3.1.3.31
TTP
inhibits SurE, YfbR, not YjjG, competitive
Escherichia coli
3.1.3.31
UTP
inhibits SurE, YfbR, and slightly YjjG, competitive
Escherichia coli
3.1.3.89
ADP
0.2 mM, 21% residual activity
Escherichia coli
3.1.3.89
ATP
0.2 mM, 18% residual activity
Escherichia coli
3.1.3.89
CTP
0.2 mM, 29% residual activity
Escherichia coli
3.1.3.89
dATP
0.2 mM, 15% residual activity
Escherichia coli
3.1.3.89
dCTP
0.2 mM, 19% residual activity
Escherichia coli
3.1.3.89
dGTP
0.2 mM, 13% residual activity
Escherichia coli
3.1.3.89
dTTP
0.2 mM, 15% residual activity
Escherichia coli
3.1.3.89
GTP
0.2 mM, 28% residual activity
Escherichia coli
3.1.3.89
ITP
0.2 mM, 29% residual activity
Escherichia coli
3.1.3.89
TTP
0.2 mM, 14% residual activity
Escherichia coli
3.1.3.89
UTP
0.2 mM, 26% residual activity
Escherichia coli
3.6.1.11
ATP
-
Escherichia coli
3.6.1.11
CTP
-
Escherichia coli
3.6.1.11
dATP
-
Escherichia coli
3.6.1.11
dCTP
-
Escherichia coli
3.6.1.11
dGTP
-
Escherichia coli
3.6.1.11
dTTP
-
Escherichia coli
3.6.1.11
GTP
-
Escherichia coli
3.6.1.11
ITP
-
Escherichia coli
3.6.1.11
TTP
-
Escherichia coli
3.6.1.11
UTP
-
Escherichia coli
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.1.3.5
1.3
-
ADP
-
Escherichia coli
3.1.3.5
1.39
-
dATP
-
Escherichia coli
3.1.3.5
1.65
-
ATP
-
Escherichia coli
3.1.3.5
24.1
-
ATP
with 3'-AMP as substrate
Escherichia coli
3.1.3.6
24.1
-
ATP
with 3'-AMP as substrate
Escherichia coli
3.1.3.31
0.0013
-
ADP
pH 8.0, 37°C, YfbR, versus 5'-dAMP, with Co2+
Escherichia coli
3.1.3.31
0.0014
-
dATP
pH 8.0, 37°C, YfbR, versus 5'-dAMP, with Co2+
Escherichia coli
3.1.3.31
0.0017
-
ATP
pH 8.0, 37°C, YfbR, versus 5'-dAMP, with Co2+
Escherichia coli
3.1.3.31
0.024
-
ATP
pH 7.0, 37°C, SurE, versus 3'-AMP, with Mn2+
Escherichia coli
3.6.1.11
24.1
-
ATP
with 3'-AMP as substrate
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.5
0.008
-
5'-dTMP
+/- 0.0003
Escherichia coli
3.1.3.5
0.012
-
5'-dAMP
+/- 0.001
Escherichia coli
3.1.3.5
0.017
-
5'-dIMP
+/- 0.001
Escherichia coli
3.1.3.5
0.02
-
5'-dUMP
+/- 0.001
Escherichia coli
3.1.3.5
0.02
-
Polyphosphate
+/- 0.003
Escherichia coli
3.1.3.5
0.036
-
5'-dCMP
+/- 0.002
Escherichia coli
3.1.3.5
0.047
-
5'-dGMP
+/- 0.005
Escherichia coli
3.1.3.5
0.1
-
3'-AMP
+/- 0.01
Escherichia coli
3.1.3.5
0.26
-
5'-GMP
+/- 0.05
Escherichia coli
3.1.3.5
0.28
-
5'-dGMP
+/- 0.04
Escherichia coli
3.1.3.5
0.32
-
5'-AMP
+/- 0.04
Escherichia coli
3.1.3.5
0.37
-
3'-CMP
+/- 0.08
Escherichia coli
3.1.3.5
2.09
-
p-nitrophenyl phosphate
+/- 0.13
Escherichia coli
3.1.3.5
2.49
-
p-nitrophenyl phosphate
+/- 0.4
Escherichia coli
3.1.3.6
0.02
-
Polyphosphate
+/- 0.003
Escherichia coli
3.1.3.6
0.1
-
3'-AMP
+/- 0.01
Escherichia coli
3.1.3.6
0.26
-
5'-GMP
+/- 0.05
Escherichia coli
3.1.3.6
0.28
-
5'-dGMP
+/- 0.04
Escherichia coli
3.1.3.6
0.32
-
5'-AMP
+/- 0.04
Escherichia coli
3.1.3.6
0.37
-
3'-CMP
+/- 0.08
Escherichia coli
3.1.3.6
2.49
-
p-nitrophenyl phosphate
+/- 0.4
Escherichia coli
3.1.3.31
0.008
-
5'-dTMP
pH 8.0, 37°C, YfbR, with Co2+
Escherichia coli
3.1.3.31
0.012
-
5'-dAMP
pH 8.0, 37°C, YfbR, with Co2+
Escherichia coli
3.1.3.31
0.017
-
5'-dIMP
pH 8.0, 37°C, YfbR, with Co2+
Escherichia coli
3.1.3.31
0.02
-
5'-dUMP
pH 8.0, 37°C, YfbR, with Co2+
Escherichia coli
3.1.3.31
0.02
-
Polyphosphate
pH 7.0, 37°C, SurE, with Mg2+
Escherichia coli
3.1.3.31
0.036
-
5'-dCMP
pH 8.0, 37°C, YfbR, with Co2+
Escherichia coli
3.1.3.31
0.047
-
5'-dGMP
pH 8.0, 37°C, YfbR, with Co2+
Escherichia coli
3.1.3.31
0.1
-
3'-AMP
pH 7.0, 37°C, SurE, with Mn2+
Escherichia coli
3.1.3.31
0.26
-
5'-GMP
pH 7.0, 37°C, SurE, with Mn2+
Escherichia coli
3.1.3.31
0.28
-
5'-dGMP
pH 7.0, 37°C, SurE, with Mn2+
Escherichia coli
3.1.3.31
0.32
-
5'-AMP
pH 7.0, 37°C, SurE, with Mn2+
Escherichia coli
3.1.3.31
0.37
-
3'-CMP
pH 7.0, 37°C, SurE, with Mn2+
Escherichia coli
3.1.3.31
0.51
-
5'-dTMP
pH 7.5, 37°C, YjjG, with Mn2+
Escherichia coli
3.1.3.31
0.66
-
5'-UMP
pH 7.5, 37°C, YjjG, with Mn2+
Escherichia coli
3.1.3.31
0.77
-
5'-dUMP
pH 7.5, 37°C, YjjG, with Mn2+
Escherichia coli
3.1.3.31
2.09
-
4-nitrophenyl phosphate
pH 8.0, 37°C, YfbR, with Co2+
Escherichia coli
3.1.3.31
2.49
-
4-nitrophenyl phosphate
pH 7.0, 37°C, SurE, with Mn2+
Escherichia coli
3.1.3.31
17.8
-
4-nitrophenyl phosphate
pH 7.5, 37°C, YjjG, with Mn2+
Escherichia coli
3.1.3.89
0.008
-
5'-dTMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
0.012
-
5'-dAMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
0.017
-
5'-dIMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
0.02
-
5'-dUMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
0.036
-
5'-dCMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
0.047
-
5'-dGMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
2.09
-
4-nitrophenyl phosphate
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.6.1.11
0.02
-
Polyphosphate
+/- 0.003
Escherichia coli
3.6.1.11
0.1
-
3'-AMP
+/- 0.01
Escherichia coli
3.6.1.11
0.26
-
5'-GMP
+/- 0.05
Escherichia coli
3.6.1.11
0.28
-
5'-dGMP
+/- 0.04
Escherichia coli
3.6.1.11
0.32
-
5'-AMP
+/- 0.04
Escherichia coli
3.6.1.11
0.37
-
3'-CMP
+/- 0.08
Escherichia coli
3.6.1.11
2.49
-
p-nitrophenyl phosphate
+/- 0.4
Escherichia coli
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.5
Co2+
activates with 5'-dAMP, KD: 67.6 +/- 2.86 micorM; activates with p-nitrophenyl phosphate, KD: 537.7 +/- 19.7 microM; KD: 46.9 +/- 5.66 microM Co2+ with p-nitrophenyl phosphate, KD: 10.7 +/- 1.07 microM Co2+ with 5'-AMP
Escherichia coli
3.1.3.5
Mg2+
KD: 224.7 +/- 35.1 microM Mg2+ with p-nitrophenyl phosphate, KD: 140.0 +/- 9.99 microM Mg2+ with 5'-AMP
Escherichia coli
3.1.3.5
Mn2+
KD: 7.24 +/- 0.71 microM Mn2+ with p-nitrophenyl phosphate, KD: 2.23 +/- 0.14 micorM Mn2+ with 5'-AMP
Escherichia coli
3.1.3.5
Ni2+
KD: 72.3 +/- 7.61 microM Ni2+ with p-nitrophenyl phosphate, KD: 29.4 +/- 3.69 microM Ni2+ with 5'-AMP
Escherichia coli
3.1.3.6
Co2+
KD: 46.9 +/- 5.66 microM Co2+ with p-nitrophenyl phosphate, KD: 10.7 +/- 1.07 microM Co2+ with 5'-AMP
Escherichia coli
3.1.3.6
Mg2+
KD: 224.7 +/- 35.1 microM Mg2+ with p-nitrophenyl phosphate, KD: 140.0 +/- 9.99 microM Mg2+ with 5'-AMP
Escherichia coli
3.1.3.6
Mn2+
KD: 7.24 +/- 0.71 microM Mn2+ with p-nitrophenyl phosphate, KD: 2.23 +/- 0.14 micorM Mn2+ with 5'-AMP
Escherichia coli
3.1.3.6
Ni2+
KD: 72.3 +/- 7.61 microM Ni2+ with p-nitrophenyl phosphate, KD: 29.4 +/- 3.69 microM Ni2+ with 5'-AMP
Escherichia coli
3.1.3.31
Co2+
preferred by YfbR, activates SurE, YfbR, and YjjG
Escherichia coli
3.1.3.31
Cu2+
activates YfbR
Escherichia coli
3.1.3.31
Mg2+
preferred by YjjG, activates SurE and YjjG
Escherichia coli
3.1.3.31
Mn2+
preferred by SurE, activates SurE, YfbR, and YjjG
Escherichia coli
3.1.3.31
additional information
dependent on divalent cations: SurE prefers Mn2+ to Co2+, Ni2+, and Mg2+, YfbR prefers Co2+ to Mn2+ and Cu2+, YjjG prefers Mg2+ to Mn2+ and Co2+, in descending orders, overview
Escherichia coli
3.1.3.31
Ni2+
activates SurE
Escherichia coli
3.1.3.89
Co2+
presence of divalent cation absolutely required. Maximum activity with Co2+, followed in decreasing order by Mn2+, Cu2+. Apparent Kd value for Co2+ 0.8 mM
Escherichia coli
3.1.3.89
Cu2+
presence of divalent cation absolutely required. Maximum activity with Co2+, followed in decreasing order by Mn2+, Cu2+
Escherichia coli
3.1.3.89
Mn2+
presence of divalent cation absolutely required. Maximum activity with Co2+, followed in decreasing order by Mn2+, Cu2+
Escherichia coli
3.6.1.11
Co2+
KD: 46.9 +/- 5.66 micorM Co2+ with p-nitrophenyl phosphate, KD: 10.7 +/- 1.07 microM Co2+ with 5'-AMP
Escherichia coli
3.6.1.11
Mg2+
KD: 224.7 +/- 35.1 microM Mg2+ with p-nitrophenyl phosphate, KD: 140.0 +/- 9.99 microM Mg2+ with 5'-AMP
Escherichia coli
3.6.1.11
Mn2+
KD: 7.24 +/- 0.71 microM Mn2+ with p-nitrophenyl phosphate, KD: 2.23 +/- 0.14 micorM Mn2+ with 5'-AMP
Escherichia coli
3.6.1.11
Ni2+
KD: 72.3 +/- 7.61 microM Ni2+ with p-nitrophenyl phosphate, KD: 29.4 +/- 3.69 microM Ni2+ with 5'-AMP
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.31
35700
-
monomeric YjjG, gel filtration
Escherichia coli
3.1.3.31
41600
-
monomeric SurE, gel filtration
Escherichia coli
3.1.3.31
52800
-
dimeric YjjG, gel filtration
Escherichia coli
3.1.3.31
71300
-
trimeric YfbR, gel filtration
Escherichia coli
3.1.3.31
100000
-
above, tetrameric YjjG and YfbR, gel filtration
Escherichia coli
3.1.3.89
24000
-
and tetramer, 3 * 24000, SDS-PAGE; and trimer, 4 * 24000, SDS-PAGE
Escherichia coli
3.1.3.89
71300
-
and above 100000, gel filtration
Escherichia coli
3.1.3.89
100000
-
and 71300, gel filtration
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.5
0.1
-
+/- 0.01, polyphosphate
Escherichia coli
3.1.3.5
0.37
-
+/- 0.01, 5'-dTMP
Escherichia coli
3.1.3.5
0.46
-
+/- 0.03, 5'-dGMP
Escherichia coli
3.1.3.5
0.52
-
+/- 0.02, 5'-dCMP
Escherichia coli
3.1.3.5
0.54
-
+/- 0.02, 5'-dUMP
Escherichia coli
3.1.3.5
0.56
-
+/- 0.01, 5'-dIMP
Escherichia coli
3.1.3.5
0.71
-
+/- 0.02, 5'-dAMP
Escherichia coli
3.1.3.5
1.32
-
+/- 0.02, p-nitrophenyl phosphate
Escherichia coli
3.1.3.5
7.24
-
+/- 0.34, p-nitrophenyl phosphate
Escherichia coli
3.1.3.5
10
-
+/- 0.61, 5'-AMP
Escherichia coli
3.1.3.5
12.1
-
+/- 0.90, 3'-CMP
Escherichia coli
3.1.3.5
16.4
-
+/- 0.80, 5'-dGMP
Escherichia coli
3.1.3.5
20.1
-
+/- 0.76, 3'-AMP
Escherichia coli
3.1.3.5
22.4
-
+/- 1.78, 5'-GMP
Escherichia coli
3.1.3.6
0.1
-
+/- 0.01, polyphosphate
Escherichia coli
3.1.3.6
7.24
-
+/- 0.34, p-nitrophenyl phosphate
Escherichia coli
3.1.3.6
10
-
+/- 0.61, 5'-AMP
Escherichia coli
3.1.3.6
12.1
-
+/- 0.90, 3'-CMP
Escherichia coli
3.1.3.6
16.4
-
+/- 0.80, 5'-dGMP
Escherichia coli
3.1.3.6
20.1
-
+/- 0.76, 3'-AMP
Escherichia coli
3.1.3.6
22.4
-
+/- 1.78, 5'-GMP
Escherichia coli
3.1.3.31
additional information
-
-
Escherichia coli
3.6.1.11
0.1
-
+/- 0.01, polyphosphate
Escherichia coli
3.6.1.11
7.24
-
+/- 0.34, p-nitrophenyl phosphate
Escherichia coli
3.6.1.11
10
-
+/- 0.61, 5'-AMP
Escherichia coli
3.6.1.11
12.1
-
+/- 0.90, 3'-CMP
Escherichia coli
3.6.1.11
16.4
-
+/- 0.80, 5'-dGMP
Escherichia coli
3.6.1.11
20.1
-
+/- 0.76, 3'-AMP
Escherichia coli
3.6.1.11
22.4
-
+/- 1.78, 5'-GMP
Escherichia coli
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.3.5
4°C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months
Escherichia coli
3.1.3.6
4°C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months
Escherichia coli
3.6.1.11
4°C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.5
(polyphosphate)n + H2O
-
656266
Escherichia coli
(polyphosphate)n-1 + phosphate
-
-
-
-
3.1.3.5
3'-AMP + H2O
-
656266
Escherichia coli
adenosine + phosphate
-
-
-
-
3.1.3.5
3'-CMP + H2O
-
656266
Escherichia coli
cytosine + phosphate
-
-
-
-
3.1.3.5
5'-AMP + H2O
-
656266
Escherichia coli
adenosine + phosphate
-
-
-
-
3.1.3.5
5'-dAMP + H2O
-
656266
Escherichia coli
deoxyadenosine + phosphate
-
-
-
-
3.1.3.5
5'-dCMP + H2O
-
656266
Escherichia coli
2'-deoxycytidine + phosphate
-
-
-
-
3.1.3.5
5'-dGMP + H2O
-
656266
Escherichia coli
deoxyguanosine + phosphate
-
-
-
-
3.1.3.5
5'-dIMP + H2O
-
656266
Escherichia coli
deoxyinosine + phosphate
-
-
-
-
3.1.3.5
5'-dTMP + H2O
-
656266
Escherichia coli
deoxythymidine + phosphate
-
-
-
-
3.1.3.5
5'-dUMP + H2O
-
656266
Escherichia coli
deoxyuridine + phosphate
-
-
-
-
3.1.3.5
5'-GMP + H2O
-
656266
Escherichia coli
guanosine + phosphate
-
-
-
-
3.1.3.5
p-nitrophenyl phosphate + H2O
-
656266
Escherichia coli
p-nitrophenol + phosphate
-
-
-
-
3.1.3.6
(polyphosphate)n + H2O
-
656266
Escherichia coli
(polyphosphate)(n-1) + phosphate
-
-
-
-
3.1.3.6
3'-AMP + H2O
-
656266
Escherichia coli
adenosine + phosphate
-
-
-
-
3.1.3.6
3'-CMP + H2O
-
656266
Escherichia coli
cytosine + phosphate
-
-
-
-
3.1.3.6
5'-AMP + H2O
-
656266
Escherichia coli
adenosine + phosphate
-
-
-
-
3.1.3.6
5'-dGMP + H2O
-
656266
Escherichia coli
deoxyguanosine + phosphate
-
-
-
-
3.1.3.6
5'-GMP + H2O
-
656266
Escherichia coli
guanosine + phosphate
-
-
-
-
3.1.3.6
p-nitrophenyl phosphate + H2O
-
656266
Escherichia coli
p-nitrophenol + phosphate
-
-
-
-
3.1.3.31
3'-AMP + H2O
-
656266
Escherichia coli
adenosine + phosphate
-
-
-
?
3.1.3.31
3'-CMP + H2O
-
656266
Escherichia coli
cytosine + phosphate
-
-
-
?
3.1.3.31
4-nitrophenyl phosphate + H2O
-
656266
Escherichia coli
4-nitrophenol + phosphate
-
-
-
?
3.1.3.31
5'-AMP + H2O
-
656266
Escherichia coli
adenosine + phosphate
-
-
-
?
3.1.3.31
5'-CMP + H2O
-
656266
Escherichia coli
cytidine + phosphate
-
-
-
?
3.1.3.31
5'-dAMP + H2O
-
656266
Escherichia coli
deoxyadenosine + phosphate
-
-
-
?
3.1.3.31
5'-dCMP + H2O
-
656266
Escherichia coli
2'-deoxycytidine + phosphate
-
-
-
?
3.1.3.31
5'-dGMP + H2O
-
656266
Escherichia coli
deoxyguanosine + phosphate
-
-
-
?
3.1.3.31
5'-dIMP + H2O
YfbR
656266
Escherichia coli
hypoxanthine + phosphate
-
-
-
?
3.1.3.31
5'-dTMP + H2O
-
656266
Escherichia coli
deoxythymidine + phosphate
-
-
-
?
3.1.3.31
5'-dUMP + H2O
-
656266
Escherichia coli
deoxyuridine + phosphate
-
-
-
-
3.1.3.31
5'-GMP + H2O
-
656266
Escherichia coli
guanosine + phosphate
-
-
-
?
3.1.3.31
5'-TMP + H2O
-
656266
Escherichia coli
thymidine + phosphate
-
-
-
?
3.1.3.31
additional information
substrate specificities of SurE, YfbR, and YjjG, overview
656266
Escherichia coli
?
-
-
-
-
3.1.3.31
polyphosphate + H2O
SurE
656266
Escherichia coli
phosphate
-
-
-
?
3.1.3.89
4-nitrophenyl phosphate + H2O
-
656266
Escherichia coli
4-nitrophenol + phosphate
-
-
-
?
3.1.3.89
5'-dAMP + H2O
-
656266
Escherichia coli
deoxyadenosine + phosphate
-
-
-
?
3.1.3.89
5'-dCMP + H2O
-
656266
Escherichia coli
deoxycytidine + phosphate
-
-
-
?
3.1.3.89
5'-dGMP + H2O
-
656266
Escherichia coli
deoxyguanosine + phosphate
-
-
-
?
3.1.3.89
5'-dIMP + H2O
-
656266
Escherichia coli
deoxyinosine + phosphate
-
-
-
?
3.1.3.89
5'-dTMP + H2O
-
656266
Escherichia coli
thymidine + phosphate
-
-
-
?
3.1.3.89
5'-dUMP + H2O
-
656266
Escherichia coli
deoxyuridine + phosphate
-
-
-
?
3.1.3.89
additional information
enzyme is strictly specific to deoxyribonucleoside 5'-monophosphates and does not hydrolyze ribonucleotides or deoxyribonucleoside 3'-monophosphates. It has similar levels of activity against all tested deoxyribonucleoside 5'-monophosphates
656266
Escherichia coli
?
-
-
-
-
3.6.1.11
(polyphosphate)n + H2O
-
656266
Escherichia coli
(polyphosphate)n-1 + phosphate
-
-
-
-
3.6.1.11
3'-AMP + H2O
-
656266
Escherichia coli
adenosine + phosphate
-
-
-
-
3.6.1.11
3'-CMP + H2O
-
656266
Escherichia coli
cytosine + phosphate
-
-
-
-
3.6.1.11
5'-AMP + H2O
-
656266
Escherichia coli
adenosine + phosphate
-
-
-
-
3.6.1.11
5'-dGMP + H2O
-
656266
Escherichia coli
deoxyguanosine + phosphate
-
-
-
-
3.6.1.11
5'-GMP + H2O
-
656266
Escherichia coli
guanosine + phosphate
-
-
-
-
3.6.1.11
p-nitrophenyl phosphate + H2O
-
656266
Escherichia coli
p-nitrophenol + phosphate
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.3.5
monomer
in solution
Escherichia coli
3.1.3.5
oligomer
at least four identical subunits in solution; three or more subunits in solution
Escherichia coli
3.1.3.6
monomer
in solution
Escherichia coli
3.1.3.6
oligomer
at least four identical subunits in solution
Escherichia coli
3.1.3.31
More
SurE, YfbR, and YjjG show monomeric, dimeric, trimeric, and tetrameric forms, overview
Escherichia coli
3.1.3.31
oligomer
x * 35700, YjjG, SDS-PAGE and gel filtration, x * 41600, SurE, SDS-PAGE and gel filtration
Escherichia coli
3.1.3.89
tetramer
and trimer, 4 * 24000, SDS-PAGE
Escherichia coli
3.1.3.89
trimer
and tetramer, 3 * 24000, SDS-PAGE
Escherichia coli
3.6.1.11
monomer
in solution
Escherichia coli
3.6.1.11
oligomer
at least four subunits in solution
Escherichia coli
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.3.31
37
-
assay at
Escherichia coli
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.1.3.5
0.05
-
Polyphosphate
+/- 0.005
Escherichia coli
3.1.3.5
0.16
-
5'-dTMP
+/- 0.01
Escherichia coli
3.1.3.5
0.2
-
5'-dGMP
+/- 0.01
Escherichia coli
3.1.3.5
0.23
-
5'-dCMP
+/- 0.01
Escherichia coli
3.1.3.5
0.23
-
5'-dUMP
+/- 0.01
Escherichia coli
3.1.3.5
0.24
-
5'-dIMP
+/- 0.01
Escherichia coli
3.1.3.5
0.3
-
5'-dAMP
+/- 0.01
Escherichia coli
3.1.3.5
0.56
-
p-nitrophenyl phosphate
+/- 0.01
Escherichia coli
3.1.3.5
3.55
-
p-nitrophenyl phosphate
+/- 0.16
Escherichia coli
3.1.3.5
4.9
-
5'-AMP
+/- 0.3
Escherichia coli
3.1.3.5
5.93
-
3'-CMP
+/- 0.44
Escherichia coli
3.1.3.5
8.04
-
5'-dGMP
+/- 0.39
Escherichia coli
3.1.3.5
9.84
-
3'-AMP
+/- 0.37
Escherichia coli
3.1.3.5
11
-
5'-GMP
+/- 0.87
Escherichia coli
3.1.3.6
0.05
-
Polyphosphate
+/- 0.005
Escherichia coli
3.1.3.6
3.55
-
p-nitrophenyl phosphate
+/- 0.16
Escherichia coli
3.1.3.6
4.9
-
5'-AMP
+/- 0.3
Escherichia coli
3.1.3.6
5.93
-
3'-CMP
+/- 0.44
Escherichia coli
3.1.3.6
8.04
-
5'-dGMP
+/- 0.39
Escherichia coli
3.1.3.6
9.84
-
3'-AMP
+/- 0.37
Escherichia coli
3.1.3.6
11
-
5'-GMP
+/- 0.87
Escherichia coli
3.1.3.31
0.05
-
Polyphosphate
pH 7.0, 37°C, SurE, with Mg2+
Escherichia coli
3.1.3.31
0.16
-
5'-dTMP
pH 8.0, 37°C, YfbR, with Co2+
Escherichia coli
3.1.3.31
0.2
-
5'-dGMP
pH 8.0, 37°C, YfbR, with Co2+
Escherichia coli
3.1.3.31
0.23
-
5'-dCMP
pH 8.0, 37°C, YfbR, with Co2+
Escherichia coli
3.1.3.31
0.23
-
5'-dUMP
pH 8.0, 37°C, YfbR, with Co2+
Escherichia coli
3.1.3.31
0.24
-
5'-dIMP
pH 8.0, 37°C, YfbR, with Co2+
Escherichia coli
3.1.3.31
0.3
-
5'-dAMP
pH 8.0, 37°C, YfbR, with Co2+
Escherichia coli
3.1.3.31
0.56
-
4-nitrophenyl phosphate
pH 8.0, 37°C, YfbR, with Co2+
Escherichia coli
3.1.3.31
3.55
-
4-nitrophenyl phosphate
pH 7.0, 37°C, SurE, with Mn2+
Escherichia coli
3.1.3.31
4.1
-
4-nitrophenyl phosphate
pH 7.5, 37°C, YjjG, with Mn2+
Escherichia coli
3.1.3.31
4.9
-
5'-AMP
pH 7.0, 37°C, SurE, with Mn2+
Escherichia coli
3.1.3.31
5.93
-
3'-CMP
pH 7.0, 37°C, SurE, with Mn2+
Escherichia coli
3.1.3.31
8.04
-
5'-dGMP
pH 7.0, 37°C, SurE, with Mn2+
Escherichia coli
3.1.3.31
9.84
-
3'-AMP
pH 7.0, 37°C, SurE, with Mn2+
Escherichia coli
3.1.3.31
11
-
5'-GMP
pH 7.0, 37°C, SurE, with Mn2+
Escherichia coli
3.1.3.31
21.4
-
5'-dUMP
pH 7.5, 37°C, YjjG, with Mn2+
Escherichia coli
3.1.3.31
30.4
-
5'-dTMP
pH 7.5, 37°C, YjjG, with Mn2+
Escherichia coli
3.1.3.31
34.2
-
5'-UMP
pH 7.5, 37°C, YjjG, with Mn2+
Escherichia coli
3.1.3.89
0.16
-
5'-dTMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
0.2
-
5'-dGMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
0.23
-
5'-dCMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
0.23
-
5'-dUMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
0.24
-
5'-dIMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
0.3
-
5'-dAMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
0.56
-
4-nitrophenyl phosphate
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.6.1.11
0.05
-
Polyphosphate
+/- 0.005
Escherichia coli
3.6.1.11
3.55
-
p-nitrophenyl phosphate
+/- 0.16
Escherichia coli
3.6.1.11
4.9
-
5'-AMP
+/- 0.3
Escherichia coli
3.6.1.11
5.93
-
3'-CMP
+/- 0.44
Escherichia coli
3.6.1.11
8.04
-
5'-dGMP
+/- 0.39
Escherichia coli
3.6.1.11
9.84
-
3'-AMP
+/- 0.37
Escherichia coli
3.6.1.11
11
-
5'-GMP
+/- 0.87
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.5
7
-
-
Escherichia coli
3.1.3.5
8
-
-
Escherichia coli
3.1.3.6
7
-
-
Escherichia coli
3.1.3.31
7
-
SurE
Escherichia coli
3.1.3.31
7.5
-
YjjG
Escherichia coli
3.1.3.31
8
-
YfbR
Escherichia coli
3.6.1.11
7
-
-
Escherichia coli
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.3.89
7
8
-
Escherichia coli
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.1.3.89
0.27
-
4-nitrophenyl phosphate
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
4.22
-
5'-dGMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
6.42
-
5'-dCMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
11.6
-
5'-dUMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
14.1
-
5'-dIMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
21.3
-
5'-dTMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
25.6
-
5'-dAMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.1.3.89
0.27
-
4-nitrophenyl phosphate
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
4.22
-
5'-dGMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
6.42
-
5'-dCMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
11.6
-
5'-dUMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
14.1
-
5'-dIMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
21.3
-
5'-dTMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli
3.1.3.89
25.6
-
5'-dAMP
presence of Co2+, pH 8.0, 37°C
Escherichia coli