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Literature summary extracted from

  • Proudfoot, M.; Kuznetsova, E.; Brown, G.; Rao, N.N.; Kitagawa, M.; Mori, H.; Savchenko, A.; Yakunin, A.F.
    General Enzymatic Screens Identify Three New Nucleotidases in Escherichia coli. Biochemical Characterization of SurE, YfbR, and Yjjg (2004), J. Biol. Chem., 279, 54687-54694.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.5
-
 Escherichia coli
3.1.3.6
-
 Escherichia coli
3.6.1.11
-
 Escherichia coli

General Stability

EC Number General Stability Organism
3.1.3.5 stable Escherichia coli
3.1.3.6 stable Escherichia coli
3.6.1.11 stable Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.5 ADP
-
 Escherichia coli
3.1.3.5 ATP
-
 Escherichia coli
3.1.3.5 CTP
-
 Escherichia coli
3.1.3.5 dATP
-
 Escherichia coli
3.1.3.5 dCTP
-
 Escherichia coli
3.1.3.5 dGTP
-
 Escherichia coli
3.1.3.5 dTTP
-
 Escherichia coli
3.1.3.5 GTP
-
 Escherichia coli
3.1.3.5 ITP
-
 Escherichia coli
3.1.3.5 TTP
-
 Escherichia coli
3.1.3.5 UTP
-
 Escherichia coli
3.1.3.6 ATP
-
 Escherichia coli
3.1.3.6 CTP
-
 Escherichia coli
3.1.3.6 dATP
-
 Escherichia coli
3.1.3.6 dCTP
-
 Escherichia coli
3.1.3.6 dGTP
-
 Escherichia coli
3.1.3.6 dTTP
-
 Escherichia coli
3.1.3.6 GTP
-
 Escherichia coli
3.1.3.6 ITP
-
 Escherichia coli
3.1.3.6 TTP
-
 Escherichia coli
3.1.3.6 UTP
-
 Escherichia coli
3.1.3.89 ADP 0.2 mM, 21% residual activity Escherichia coli
3.1.3.89 ATP 0.2 mM, 18% residual activity Escherichia coli
3.1.3.89 CTP 0.2 mM, 29% residual activity Escherichia coli
3.1.3.89 dATP 0.2 mM, 15% residual activity Escherichia coli
3.1.3.89 dCTP 0.2 mM, 19% residual activity Escherichia coli
3.1.3.89 dGTP 0.2 mM, 13% residual activity Escherichia coli
3.1.3.89 dTTP 0.2 mM, 15% residual activity Escherichia coli
3.1.3.89 GTP 0.2 mM, 28% residual activity Escherichia coli
3.1.3.89 ITP 0.2 mM, 29% residual activity Escherichia coli
3.1.3.89 TTP 0.2 mM, 14% residual activity Escherichia coli
3.1.3.89 UTP 0.2 mM, 26% residual activity Escherichia coli
3.6.1.11 ATP
-
 Escherichia coli
3.6.1.11 CTP
-
 Escherichia coli
3.6.1.11 dATP
-
 Escherichia coli
3.6.1.11 dCTP
-
 Escherichia coli
3.6.1.11 dGTP
-
 Escherichia coli
3.6.1.11 dTTP
-
 Escherichia coli
3.6.1.11 GTP
-
 Escherichia coli
3.6.1.11 ITP
-
 Escherichia coli
3.6.1.11 TTP
-
 Escherichia coli
3.6.1.11 UTP
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.5 0.008
-
 5'-dTMP +/- 0.0003 Escherichia coli
3.1.3.5 0.012
-
 5'-dAMP +/- 0.001 Escherichia coli
3.1.3.5 0.017
-
 5'-dIMP +/- 0.001 Escherichia coli
3.1.3.5 0.02
-
 5'-dUMP +/- 0.001 Escherichia coli
3.1.3.5 0.02
-
 (Polyphosphate)n +/- 0.003 Escherichia coli
3.1.3.5 0.036
-
 5'-dCMP +/- 0.002 Escherichia coli
3.1.3.5 0.047
-
 5'-dGMP +/- 0.005 Escherichia coli
3.1.3.5 0.1
-
 3'-AMP +/- 0.01 Escherichia coli
3.1.3.5 0.26
-
 5'-GMP +/- 0.05 Escherichia coli
3.1.3.5 0.28
-
 5'-dGMP +/- 0.04 Escherichia coli
3.1.3.5 0.32
-
 5'-AMP +/- 0.04 Escherichia coli
3.1.3.5 0.37
-
 3'-CMP +/- 0.08 Escherichia coli
3.1.3.5 2.09
-
 4-nitrophenyl phosphate +/- 0.13 Escherichia coli
3.1.3.5 2.49
-
 4-nitrophenyl phosphate +/- 0.4 Escherichia coli
3.1.3.6 0.02
-
 (Polyphosphate)n +/- 0.003 Escherichia coli
3.1.3.6 0.1
-
 3'-AMP +/- 0.01 Escherichia coli
3.1.3.6 0.26
-
 5'-GMP +/- 0.05 Escherichia coli
3.1.3.6 0.28
-
 5'-dGMP +/- 0.04 Escherichia coli
3.1.3.6 0.32
-
 5'-AMP +/- 0.04 Escherichia coli
3.1.3.6 0.37
-
 3'-CMP +/- 0.08 Escherichia coli
3.1.3.6 2.49
-
 p-nitrophenyl phosphate +/- 0.4 Escherichia coli
3.1.3.89 0.008
-
 5'-dTMP presence of Co2+, pH 8.0, 37°C Escherichia coli
3.1.3.89 0.012
-
 5'-dAMP presence of Co2+, pH 8.0, 37°C Escherichia coli
3.1.3.89 0.017
-
 5'-dIMP presence of Co2+, pH 8.0, 37°C Escherichia coli
3.1.3.89 0.02
-
 5'-dUMP presence of Co2+, pH 8.0, 37°C Escherichia coli
3.1.3.89 0.036
-
 5'-dCMP presence of Co2+, pH 8.0, 37°C Escherichia coli
3.1.3.89 0.047
-
 5'-dGMP presence of Co2+, pH 8.0, 37°C Escherichia coli
3.1.3.89 2.09
-
 4-nitrophenyl phosphate presence of Co2+, pH 8.0, 37°C Escherichia coli
3.6.1.11 0.02
-
 (Polyphosphate)n +/- 0.003 Escherichia coli
3.6.1.11 0.1
-
 3'-AMP +/- 0.01 Escherichia coli
3.6.1.11 0.26
-
 5'-GMP +/- 0.05 Escherichia coli
3.6.1.11 0.28
-
 5'-dGMP +/- 0.04 Escherichia coli
3.6.1.11 0.32
-
 5'-AMP +/- 0.04 Escherichia coli
3.6.1.11 0.37
-
 3'-CMP +/- 0.08 Escherichia coli
3.6.1.11 2.49
-
 p-nitrophenyl phosphate +/- 0.4 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.5 Co2+ activates with 5'-dAMP, KD: 67.6 +/- 2.86 micorM Escherichia coli
3.1.3.5 Co2+ activates with p-nitrophenyl phosphate, KD: 537.7 +/- 19.7 microM Escherichia coli
3.1.3.5 Co2+ KD: 46.9 +/- 5.66 microM Co2+ with p-nitrophenyl phosphate, KD: 10.7 +/- 1.07 microM Co2+ with 5'-AMP Escherichia coli
3.1.3.5 Mg2+ KD: 224.7 +/- 35.1 microM Mg2+ with p-nitrophenyl phosphate, KD: 140.0 +/- 9.99 microM Mg2+ with 5'-AMP Escherichia coli
3.1.3.5 Mn2+ KD: 7.24 +/- 0.71 microM Mn2+ with p-nitrophenyl phosphate, KD: 2.23 +/- 0.14 micorM Mn2+ with 5'-AMP Escherichia coli
3.1.3.5 Ni2+ KD: 72.3 +/- 7.61 microM Ni2+ with p-nitrophenyl phosphate, KD: 29.4 +/- 3.69 microM Ni2+ with 5'-AMP Escherichia coli
3.1.3.6 Co2+ KD: 46.9 +/- 5.66 microM Co2+ with p-nitrophenyl phosphate, KD: 10.7 +/- 1.07 microM Co2+ with 5'-AMP Escherichia coli
3.1.3.6 Mg2+ KD: 224.7 +/- 35.1 microM Mg2+ with p-nitrophenyl phosphate, KD: 140.0 +/- 9.99 microM Mg2+ with 5'-AMP Escherichia coli
3.1.3.6 Mn2+ KD: 7.24 +/- 0.71 microM Mn2+ with p-nitrophenyl phosphate, KD: 2.23 +/- 0.14 micorM Mn2+ with 5'-AMP Escherichia coli
3.1.3.6 Ni2+ KD: 72.3 +/- 7.61 microM Ni2+ with p-nitrophenyl phosphate, KD: 29.4 +/- 3.69 microM Ni2+ with 5'-AMP Escherichia coli
3.1.3.89 Co2+ presence of divalent cation absolutely required. Maximum activity with Co2+, followed in decreasing order by Mn2+, Cu2+. Apparent Kd value for Co2+ 0.8 mM Escherichia coli
3.1.3.89 Cu2+ presence of divalent cation absolutely required. Maximum activity with Co2+, followed in decreasing order by Mn2+, Cu2+ Escherichia coli
3.1.3.89 Mn2+ presence of divalent cation absolutely required. Maximum activity with Co2+, followed in decreasing order by Mn2+, Cu2+ Escherichia coli
3.6.1.11 Co2+ KD: 46.9 +/- 5.66 micorM Co2+ with p-nitrophenyl phosphate, KD: 10.7 +/- 1.07 microM Co2+ with 5'-AMP Escherichia coli
3.6.1.11 Mg2+ KD: 224.7 +/- 35.1 microM Mg2+ with p-nitrophenyl phosphate, KD: 140.0 +/- 9.99 microM Mg2+ with 5'-AMP Escherichia coli
3.6.1.11 Mn2+ KD: 7.24 +/- 0.71 microM Mn2+ with p-nitrophenyl phosphate, KD: 2.23 +/- 0.14 micorM Mn2+ with 5'-AMP Escherichia coli
3.6.1.11 Ni2+ KD: 72.3 +/- 7.61 microM Ni2+ with p-nitrophenyl phosphate, KD: 29.4 +/- 3.69 microM Ni2+ with 5'-AMP Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.89 24000
-
 and tetramer, 3 * 24000, SDS-PAGE Escherichia coli
3.1.3.89 24000
-
 and trimer, 4 * 24000, SDS-PAGE Escherichia coli
3.1.3.89 71300
-
 and above 100000, gel filtration Escherichia coli
3.1.3.89 100000
-
 and 71300, gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Escherichia coli P0A840
-
-
3.1.3.5 Escherichia coli P76491
-
-
3.1.3.6 Escherichia coli P0A840
-
-
3.1.3.89 Escherichia coli
-
-
-
3.6.1.11 Escherichia coli P0A840
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.5 0.1
-
 +/- 0.01, polyphosphate Escherichia coli
3.1.3.5 0.37
-
 +/- 0.01, 5'-dTMP Escherichia coli
3.1.3.5 0.46
-
 +/- 0.03, 5'-dGMP Escherichia coli
3.1.3.5 0.52
-
 +/- 0.02, 5'-dCMP Escherichia coli
3.1.3.5 0.54
-
 +/- 0.02, 5'-dUMP Escherichia coli
3.1.3.5 0.56
-
 +/- 0.01, 5'-dIMP Escherichia coli
3.1.3.5 0.71
-
 +/- 0.02, 5'-dAMP Escherichia coli
3.1.3.5 1.32
-
 +/- 0.02, p-nitrophenyl phosphate Escherichia coli
3.1.3.5 7.24
-
 +/- 0.34, p-nitrophenyl phosphate Escherichia coli
3.1.3.5 10
-
 +/- 0.61, 5'-AMP Escherichia coli
3.1.3.5 12.1
-
 +/- 0.90, 3'-CMP Escherichia coli
3.1.3.5 16.4
-
 +/- 0.80, 5'-dGMP Escherichia coli
3.1.3.5 20.1
-
 +/- 0.76, 3'-AMP Escherichia coli
3.1.3.5 22.4
-
 +/- 1.78, 5'-GMP Escherichia coli
3.1.3.6 0.1
-
 +/- 0.01, polyphosphate Escherichia coli
3.1.3.6 7.24
-
 +/- 0.34, p-nitrophenyl phosphate Escherichia coli
3.1.3.6 10
-
 +/- 0.61, 5'-AMP Escherichia coli
3.1.3.6 12.1
-
 +/- 0.90, 3'-CMP Escherichia coli
3.1.3.6 16.4
-
 +/- 0.80, 5'-dGMP Escherichia coli
3.1.3.6 20.1
-
 +/- 0.76, 3'-AMP Escherichia coli
3.1.3.6 22.4
-
 +/- 1.78, 5'-GMP Escherichia coli
3.6.1.11 0.1
-
 +/- 0.01, polyphosphate Escherichia coli
3.6.1.11 7.24
-
 +/- 0.34, p-nitrophenyl phosphate Escherichia coli
3.6.1.11 10
-
 +/- 0.61, 5'-AMP Escherichia coli
3.6.1.11 12.1
-
 +/- 0.90, 3'-CMP Escherichia coli
3.6.1.11 16.4
-
 +/- 0.80, 5'-dGMP Escherichia coli
3.6.1.11 20.1
-
 +/- 0.76, 3'-AMP Escherichia coli
3.6.1.11 22.4
-
 +/- 1.78, 5'-GMP Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.1.3.5 4°C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months Escherichia coli
3.1.3.6 4°C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months Escherichia coli
3.6.1.11 4°C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.5 (polyphosphate)n + H2O
-
 Escherichia coli (polyphosphate)n-1 + phosphate
-
 ?
3.1.3.5 (polyphosphate)n + H2O
-
 Escherichia coli (polyphosphate)(n-1) + phosphate
-
 ?
3.1.3.5 3'-AMP + H2O
-
 Escherichia coli adenosine + phosphate
-
 ?
3.1.3.5 3'-CMP + H2O
-
 Escherichia coli cytosine + phosphate
-
 ?
3.1.3.5 4-nitrophenyl phosphate + H2O
-
 Escherichia coli 4-nitrophenol + phosphate
-
 ?
3.1.3.5 5'-AMP + H2O
-
 Escherichia coli adenosine + phosphate
-
 ?
3.1.3.5 5'-dAMP + H2O
-
 Escherichia coli deoxyadenosine + phosphate
-
 ?
3.1.3.5 5'-dCMP + H2O
-
 Escherichia coli deoxycytidine + phosphate
-
 ?
3.1.3.5 5'-dCMP + H2O
-
 Escherichia coli 2'-deoxycytidine + phosphate
-
 ?
3.1.3.5 5'-dGMP + H2O
-
 Escherichia coli deoxyguanosine + phosphate
-
 ?
3.1.3.5 5'-dIMP + H2O
-
 Escherichia coli deoxyinosine + phosphate
-
 ?
3.1.3.5 5'-dTMP + H2O
-
 Escherichia coli deoxythymidine + phosphate
-
 ?
3.1.3.5 5'-dUMP + H2O
-
 Escherichia coli deoxyuridine + phosphate
-
 ?
3.1.3.5 5'-GMP + H2O
-
 Escherichia coli guanosine + phosphate
-
 ?
3.1.3.5 p-nitrophenyl phosphate + H2O
-
 Escherichia coli p-nitrophenol + phosphate
-
 ?
3.1.3.6 (polyphosphate)n + H2O
-
 Escherichia coli (polyphosphate)(n-1) + phosphate
-
 ?
3.1.3.6 3'-AMP + H2O
-
 Escherichia coli adenosine + phosphate
-
 ?
3.1.3.6 3'-CMP + H2O
-
 Escherichia coli cytosine + phosphate
-
 ?
3.1.3.6 5'-AMP + H2O
-
 Escherichia coli adenosine + phosphate
-
 ?
3.1.3.6 5'-dGMP + H2O
-
 Escherichia coli deoxyguanosine + phosphate
-
 ?
3.1.3.6 5'-GMP + H2O
-
 Escherichia coli guanosine + phosphate
-
 ?
3.1.3.6 p-nitrophenyl phosphate + H2O
-
 Escherichia coli p-nitrophenol + phosphate
-
 ?
3.1.3.89 4-nitrophenyl phosphate + H2O
-
 Escherichia coli 4-nitrophenol + phosphate
-
 ?
3.1.3.89 5'-dAMP + H2O
-
 Escherichia coli deoxyadenosine + phosphate
-
 ?
3.1.3.89 5'-dCMP + H2O
-
 Escherichia coli deoxycytidine + phosphate
-
 ?
3.1.3.89 5'-dGMP + H2O
-
 Escherichia coli deoxyguanosine + phosphate
-
 ?
3.1.3.89 5'-dIMP + H2O
-
 Escherichia coli deoxyinosine + phosphate
-
 ?
3.1.3.89 5'-dTMP + H2O
-
 Escherichia coli thymidine + phosphate
-
 ?
3.1.3.89 5'-dUMP + H2O
-
 Escherichia coli deoxyuridine + phosphate
-
 ?
3.1.3.89 additional information enzyme is strictly specific to deoxyribonucleoside 5'-monophosphates and does not hydrolyze ribonucleotides or deoxyribonucleoside 3'-monophosphates. It has similar levels of activity against all tested deoxyribonucleoside 5'-monophosphates Escherichia coli ?
-
 ?
3.6.1.11 (polyphosphate)n + H2O
-
 Escherichia coli (polyphosphate)n-1 + phosphate
-
 ?
3.6.1.11 3'-AMP + H2O
-
 Escherichia coli adenosine + phosphate
-
 ?
3.6.1.11 3'-CMP + H2O
-
 Escherichia coli cytosine + phosphate
-
 ?
3.6.1.11 5'-AMP + H2O
-
 Escherichia coli adenosine + phosphate
-
 ?
3.6.1.11 5'-dGMP + H2O
-
 Escherichia coli deoxyguanosine + phosphate
-
 ?
3.6.1.11 5'-GMP + H2O
-
 Escherichia coli guanosine + phosphate
-
 ?
3.6.1.11 p-nitrophenyl phosphate + H2O
-
 Escherichia coli p-nitrophenol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.5 monomer in solution Escherichia coli
3.1.3.5 oligomer at least four identical subunits in solution Escherichia coli
3.1.3.5 oligomer three or more subunits in solution Escherichia coli
3.1.3.6 monomer in solution Escherichia coli
3.1.3.6 oligomer at least four identical subunits in solution Escherichia coli
3.1.3.89 tetramer and trimer, 4 * 24000, SDS-PAGE Escherichia coli
3.1.3.89 trimer and tetramer, 3 * 24000, SDS-PAGE Escherichia coli
3.6.1.11 monomer in solution Escherichia coli
3.6.1.11 oligomer at least four subunits in solution Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.1.3.89 YfbR
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.5 0.05
-
 (Polyphosphate)n +/- 0.005 Escherichia coli
3.1.3.5 0.16
-
 5'-dTMP +/- 0.01 Escherichia coli
3.1.3.5 0.2
-
 5'-dGMP +/- 0.01 Escherichia coli
3.1.3.5 0.23
-
 5'-dCMP +/- 0.01 Escherichia coli
3.1.3.5 0.23
-
 5'-dUMP +/- 0.01 Escherichia coli
3.1.3.5 0.24
-
 5'-dIMP +/- 0.01 Escherichia coli
3.1.3.5 0.3
-
 5'-dAMP +/- 0.01 Escherichia coli
3.1.3.5 0.56
-
 p-nitrophenyl phosphate +/- 0.01 Escherichia coli
3.1.3.5 3.55
-
 4-nitrophenyl phosphate +/- 0.16 Escherichia coli
3.1.3.5 4.9
-
 5'-AMP +/- 0.3 Escherichia coli
3.1.3.5 5.93
-
 3'-CMP +/- 0.44 Escherichia coli
3.1.3.5 8.04
-
 5'-dGMP +/- 0.39 Escherichia coli
3.1.3.5 9.84
-
 3'-AMP +/- 0.37 Escherichia coli
3.1.3.5 11
-
 5'-GMP +/- 0.87 Escherichia coli
3.1.3.6 0.05
-
 (Polyphosphate)n +/- 0.005 Escherichia coli
3.1.3.6 3.55
-
 p-nitrophenyl phosphate +/- 0.16 Escherichia coli
3.1.3.6 4.9
-
 5'-AMP +/- 0.3 Escherichia coli
3.1.3.6 5.93
-
 3'-CMP +/- 0.44 Escherichia coli
3.1.3.6 8.04
-
 5'-dGMP +/- 0.39 Escherichia coli
3.1.3.6 9.84
-
 3'-AMP +/- 0.37 Escherichia coli
3.1.3.6 11
-
 5'-GMP +/- 0.87 Escherichia coli
3.1.3.89 0.16
-
 5'-dTMP presence of Co2+, pH 8.0, 37°C Escherichia coli
3.1.3.89 0.2
-
 5'-dGMP presence of Co2+, pH 8.0, 37°C Escherichia coli
3.1.3.89 0.23
-
 5'-dCMP presence of Co2+, pH 8.0, 37°C Escherichia coli
3.1.3.89 0.23
-
 5'-dUMP presence of Co2+, pH 8.0, 37°C Escherichia coli
3.1.3.89 0.24
-
 5'-dIMP presence of Co2+, pH 8.0, 37°C Escherichia coli
3.1.3.89 0.3
-
 5'-dAMP presence of Co2+, pH 8.0, 37°C Escherichia coli
3.1.3.89 0.56
-
 4-nitrophenyl phosphate presence of Co2+, pH 8.0, 37°C Escherichia coli
3.6.1.11 0.05
-
 (Polyphosphate)n +/- 0.005 Escherichia coli
3.6.1.11 3.55
-
 p-nitrophenyl phosphate +/- 0.16 Escherichia coli
3.6.1.11 4.9
-
 5'-AMP +/- 0.3 Escherichia coli
3.6.1.11 5.93
-
 3'-CMP +/- 0.44 Escherichia coli
3.6.1.11 8.04
-
 5'-dGMP +/- 0.39 Escherichia coli
3.6.1.11 9.84
-
 3'-AMP +/- 0.37 Escherichia coli
3.6.1.11 11
-
 5'-GMP +/- 0.87 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.5 7
-
-
 Escherichia coli
3.1.3.5 8
-
-
 Escherichia coli
3.1.3.6 7
-
-
 Escherichia coli
3.6.1.11 7
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.89 7 8
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.5 1.3
-
 ADP
-
 Escherichia coli
3.1.3.5 1.39
-
 dATP
-
 Escherichia coli
3.1.3.5 1.65
-
 ATP
-
 Escherichia coli
3.1.3.5 24.1
-
 ATP with 3'-AMP as substrate Escherichia coli
3.1.3.6 24.1
-
 ATP with 3'-AMP as substrate Escherichia coli
3.6.1.11 24.1
-
 ATP with 3'-AMP as substrate Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.89 0.27
-
 4-nitrophenyl phosphate presence of Co2+, pH 8.0, 37°C Escherichia coli
3.1.3.89 4.22
-
 5'-dGMP presence of Co2+, pH 8.0, 37°C Escherichia coli
3.1.3.89 6.42
-
 5'-dCMP presence of Co2+, pH 8.0, 37°C Escherichia coli
3.1.3.89 11.6
-
 5'-dUMP presence of Co2+, pH 8.0, 37°C Escherichia coli
3.1.3.89 14.1
-
 5'-dIMP presence of Co2+, pH 8.0, 37°C Escherichia coli
3.1.3.89 21.3
-
 5'-dTMP presence of Co2+, pH 8.0, 37°C Escherichia coli
3.1.3.89 25.6
-
 5'-dAMP presence of Co2+, pH 8.0, 37°C Escherichia coli