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Literature summary extracted from

  • Chen, S.; Yakunin, A.F.; Kuznetsova, E.; Busso, D.; Pufan, R.; Proudfoot, M.; Kim, R.; Kim, S.H.
    Structural and Functional Characterization of a Novel Phosphodiesterase from Methanococcus jannaschii (2004), J. Biol. Chem., 279, 31854-31862.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.1
-
 Methanocaldococcus jannaschii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.4.1
-
 Methanocaldococcus jannaschii

General Stability

EC Number General Stability Organism
3.1.4.1 stable Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.1 PO43- competitive Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.1 0.035
-
 p-nitrophenyl 5'-thymidine monophosphate +/- 0.003, with 1 mM Ni2+ Methanocaldococcus jannaschii
3.1.4.1 0.15
-
 bis-p-nitrophenyl phosphate +/- 0.01, with 1 mM Ni2+ Methanocaldococcus jannaschii
3.1.4.1 0.66
-
 bis-p-nitrophenyl phosphate +/- 0.09, with 20 mM PO43- and 1 mM Ni2+ Methanocaldococcus jannaschii
3.1.4.1 1.01
-
 bis-p-nitrophenyl phosphate +/- 0.13, with 50 mM PO43- and 1 mM Ni2+ Methanocaldococcus jannaschii
3.1.4.1 1.53
-
 p-Nitrophenylphosphorylcholine +/- 0.1, with 20 mM Mn2+ Methanocaldococcus jannaschii
3.1.4.1 3.33
-
 p-Nitrophenylphosphorylcholine +/- 0.5, with 1 mM Ni2+ Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.1 Mn2+ activates, KD: 1.3 +/- 0.1 uM Methanocaldococcus jannaschii
3.1.4.1 Ni2+ activates, KD: 0.28 +/- 0.09 mM Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.1 Methanocaldococcus jannaschii Q58346
-
-

Storage Stability

EC Number Storage Stability Organism
3.1.4.1 4°C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.1 bis-p-nitrophenyl phosphate + H2O
-
 Methanocaldococcus jannaschii p-nitrophenol + p-nitrophenyl phosphate
-
 ?
3.1.4.1 p-nitrophenyl 5'-thymidine monophosphate + H2O
-
 Methanocaldococcus jannaschii 5'-TMP + p-nitrophenol
-
 ?
3.1.4.1 p-nitrophenylphosphorylcholine + H2O
-
 Methanocaldococcus jannaschii p-nitrophenol + phosphorylcholine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.1 monomer crystallization Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
3.1.4.1 phosphodiesterase
-
 Methanocaldococcus jannaschii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.4.1 3.26
-
 p-nitrophenyl 5'-thymidine monophosphate +/- 0.006, with 1mM Ni2+ Methanocaldococcus jannaschii
3.1.4.1 8.6
-
 p-Nitrophenylphosphorylcholine +/- 0.5, with 1 mM Ni2+ Methanocaldococcus jannaschii
3.1.4.1 52.9
-
 p-Nitrophenylphosphorylcholine +/- 1.0, with 20 mM Mn2+ Methanocaldococcus jannaschii
3.1.4.1 105.7
-
 bis-p-nitrophenyl phosphate +/- 2.8, with 1 mM Ni2+ Methanocaldococcus jannaschii
3.1.4.1 125.9
-
 bis-p-nitrophenyl phosphate +/- 8.1, with 20 mM PO43- and 1 mM Ni2+ Methanocaldococcus jannaschii
3.1.4.1 130.2
-
 bis-p-nitrophenyl phosphate +/- 8.6, with 50 mM PO43- and 1 mM Ni2+ Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.1 9.4 9.8
-
 Methanocaldococcus jannaschii