Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kovari, J.; Barabas, O.; Takacs, E.; Bekesi, A.; Dubrovay, Z.; Pongracz, V.; Zagyva, I.; Imre, T.; Szabo, P.; Vertessy, B.G.
    Altered active site flexibility and a structural metal-binding site in eukaryotic dUTPase: kinetic characterization, folding, and crystallographic studies of the homotrimeric Drosophila enzyme (2004), J. Biol. Chem., 279, 17932-17944.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.23 expressed in different Escherichia coli strains Drosophila melanogaster

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.23 hanging and sitting drop vapor diffusion method, stable monomers observed in crystal phase Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.23 DELTA160-187 truncated form Drosophila melanogaster

General Stability

EC Number General Stability Organism
3.6.1.23 dUMP protects against tryptic digestion, slight fragmentation caused by frequent freezing and thawing that does not affect the activity Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.23 dUDP
-
 Drosophila melanogaster
3.6.1.23 dUMP
-
 Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.23 0.0004
-
 dUTP pH 7.5, 25°C Drosophila melanogaster
3.6.1.23 0.64
-
 dTTP pH 7.5, 25°C Drosophila melanogaster
3.6.1.23 2.3
-
 dCTP pH 7.5, 25°C Drosophila melanogaster

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.23 Mg2+ required, modulates conformation of the enzyme Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.23 17200
-
 3 * 19800, full length enzyme, 3 * 17200, truncated enzyme, mass spectrometry, gel filtration Drosophila melanogaster
3.6.1.23 19800
-
 3 * 19800, full length enzyme, 3 * 17200, truncated enzyme, mass spectrometry, gel filtration Drosophila melanogaster
3.6.1.23 54000
-
 truncated enzyme, gel filtration Drosophila melanogaster
3.6.1.23 65000
-
 full length enzyme, gel filtration Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.23 dUTP + H2O Drosophila melanogaster removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA, provides substrate for dTTP synthesis dUMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.23 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.23 recombinant protein Drosophila melanogaster

Storage Stability

EC Number Storage Stability Organism
3.6.1.23 -70°C, stable for at least 3 months Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.23 dCTP + H2O
-
 Drosophila melanogaster dCMP + diphosphate
-
 ?
3.6.1.23 dTTP + H2O
-
 Drosophila melanogaster dTMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O
-
 Drosophila melanogaster dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA, provides substrate for dTTP synthesis Drosophila melanogaster dUMP + diphosphate
-
 ?
3.6.1.23 additional information no hydrolysis of dUDP Drosophila melanogaster ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.23 trimer 3 * 19800, full length enzyme, 3 * 17200, truncated enzyme, mass spectrometry, gel filtration Drosophila melanogaster

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.23 54
-
 melting point Drosophila melanogaster

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.23 0.1
-
 dTTP pH 7.5, 25°C Drosophila melanogaster
3.6.1.23 0.7
-
 dCTP pH 7.5, 25°C Drosophila melanogaster
3.6.1.23 12
-
 dUTP pH 7.5, 25°C Drosophila melanogaster