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Literature summary extracted from

  • Lamble, H.J.; Heyer, N.I.; Bull, S.D.; Hough, D.W.; Danson, M.J.
    Metabolic pathway promiscuity in the archaeon Sulfolobus solfataricus revealed by studies on glucose dehydrogenase and 2-keto-3-deoxygluconate aldolase (2003), J. Biol. Chem., 278, 34066-34072.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.47 DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli Saccharolobus solfataricus
1.1.1.359 expression in Escherichia coli Saccharolobus solfataricus
4.1.2.55 expression in Escherichia coli Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.47 EDTA 60% inhibition at 10 mM Saccharolobus solfataricus
1.1.1.359 EDTA 10 mM, 60% loss of activity Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.47 0.44
-
 NADP+ with beta-D-galactose, pH 7.5, 70°C Saccharolobus solfataricus
1.1.1.47 0.57
-
 NAD+ with beta-D-galactose, pH 7.5, 70°C Saccharolobus solfataricus
1.1.1.47 1.3
-
 NADP+ with beta-D-glucose, pH 7.5, 70°C Saccharolobus solfataricus
1.1.1.47 1.5
-
 NAD+ with beta-D-glucose, pH 7.5, 70°C Saccharolobus solfataricus
1.1.1.359 0.44
-
 D-galactose pH 7.5, 70°C, cofactor: NADP+ Saccharolobus solfataricus
1.1.1.359 0.57
-
 D-galactose pH 7.5, 70°C, cofactor: NAD+ Saccharolobus solfataricus
1.1.1.359 1.3
-
 beta-D-glucose pH 7.5, 70°C, cofactor: NADP+ Saccharolobus solfataricus
1.1.1.359 1.5
-
 beta-D-glucose pH 7.5, 70°C, cofactor: NAD+ Saccharolobus solfataricus
4.1.2.55 9.9
-
 2-dehydro-3-deoxy-D-galactonate pH 6.0, 60°C Saccharolobus solfataricus
4.1.2.55 25.7
-
 2-dehydro-3-deoxy-D-gluconate pH 6.0, 60°C Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.47 additional information not affected by 0.1 mM CaCl2 and MgCl2 Saccharolobus solfataricus
1.1.1.47 Zn2+ enzyme contains the catalytic zinc binding residues Cys39 and His66, no increase in activity by addition of 0.1 mM ZnCl2 Saccharolobus solfataricus
1.1.1.359 additional information no significant increase in the presence of ZnCl2, CaCl2, or MgCl2, at final concentrations of 0.1 mM Saccharolobus solfataricus
1.1.1.359 Zinc the enzyme possess the catalytic zinc binding residues Cys-39 and His-66 Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.47 160000
-
-
 Saccharolobus solfataricus
1.1.1.359 40849
-
 4 * 40849, calculated from sequence Saccharolobus solfataricus
1.1.1.359 41000
-
 4 * 41000, SDS-PAGE Saccharolobus solfataricus
1.1.1.359 160000
-
 gel filtration Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.47 beta-D-glucose + NAD(P)+ Saccharolobus solfataricus first step of the non-phosphorylative Entner-Doudoroff pathway D-glucono-1,5-lactone + NAD(P)H + H+
-
 ir
4.1.2.55 2-dehydro-3-deoxy-D-galactonate Saccharolobus solfataricus the same enzyme is responsible for the catabolism of both glucose and galactose pyruvate + D-glyceraldehyde
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-gluconate Saccharolobus solfataricus the same enzyme is responsible for the catabolism of both glucose and galactose pyruvate + D-glyceraldehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.47 Saccharolobus solfataricus O93715
-
-
1.1.1.359 Saccharolobus solfataricus O93715
-
-
4.1.2.55 Saccharolobus solfataricus O54288
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.47 native enzyme by gel filtration, recombinant enzyme from Escherichia coli by heat denaturation, and affinity gel chromatography Saccharolobus solfataricus
1.1.1.359 enzyme from Sulfolobus solfataricus cell extract and recombinant enzyme expressed in Escherichia coli Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.47 2-deoxy-D-glucose + NAD(P)+ low activity Saccharolobus solfataricus ?
-
 ?
1.1.1.47 6-deoxy-D-glucose + NAD(P)+
-
 Saccharolobus solfataricus ?
-
 ?
1.1.1.47 beta-D-glucose + NAD(P)+
-
 Saccharolobus solfataricus D-glucono-1,5-lactone + NAD(P)H + H+
-
 ir
1.1.1.47 beta-D-glucose + NAD(P)+ first step of the non-phosphorylative Entner-Doudoroff pathway Saccharolobus solfataricus D-glucono-1,5-lactone + NAD(P)H + H+
-
 ir
1.1.1.47 D-fucose + NAD(P)+
-
 Saccharolobus solfataricus ?
-
 ?
1.1.1.47 D-galactose + NAD(P)+ equally active compared to beta-D-glucose Saccharolobus solfataricus D-galactono-1,5-lactone + NAD(P)H
-
 ?
1.1.1.47 D-glucosamine + NAD(P)+ low activity Saccharolobus solfataricus ?
-
 ?
1.1.1.47 D-idose + NAD(P)+ low activity Saccharolobus solfataricus ?
-
 ?
1.1.1.47 D-lyxose + NAD(P)+ low activity Saccharolobus solfataricus ?
-
 ?
1.1.1.47 D-xylose + NAD(P)+
-
 Saccharolobus solfataricus ?
-
 ?
1.1.1.47 L-arabinose + NAD(P)+
-
 Saccharolobus solfataricus ?
-
 ?
1.1.1.47 additional information D-ribose, maltose, lactose and sucrose are poor substrates, no activity with D-allose, D-arabinose, and D-mannose Saccharolobus solfataricus ?
-
 ?
1.1.1.359 6-deoxy-D-glucose + NAD+ + H2O
-
 Saccharolobus solfataricus ?
-
 ?
1.1.1.359 6-deoxy-D-glucose + NADP+ + H2O
-
 Saccharolobus solfataricus ?
-
 ?
1.1.1.359 beta-D-glucose + NAD+ + H2O kcat/Km for beta-D-glucose in the reaction with NAD+ is 1.4fold higher than the kcat/Km value for beta-D-glucose in the reaction with NADP+ Saccharolobus solfataricus D-gluconate + NADH + 2 H+
-
 ir
1.1.1.359 beta-D-glucose + NADP+ + H2O kcat/Km for beta-D-glucose in the reaction with NADP+ is 1.4fold lower than the kcat/Km value for beta-D-glucose in the reaction with NAD+ Saccharolobus solfataricus D-gluconate + NADPH + 2 H+
-
 ir
1.1.1.359 D-fucose + NAD+ + H2O
-
 Saccharolobus solfataricus ?
-
 ?
1.1.1.359 D-fucose + NADP+ + H2O
-
 Saccharolobus solfataricus ?
-
 ?
1.1.1.359 D-galactose + NAD+ + H2O
-
 Saccharolobus solfataricus D-galactonate + NADH + 2 H+
-
 ?
1.1.1.359 D-galactose + NADP+ + H2O
-
 Saccharolobus solfataricus D-galactonate + NADPH + 2 H+
-
 ?
1.1.1.359 D-xylose + NAD+ + H2O
-
 Saccharolobus solfataricus ?
-
 ?
1.1.1.359 D-xylose + NADP+ + H2O
-
 Saccharolobus solfataricus ?
-
 ?
1.1.1.359 L-arabinose + NAD+ + H2O
-
 Saccharolobus solfataricus ?
-
 ?
1.1.1.359 L-arabinose + NADP+ + H2O
-
 Saccharolobus solfataricus ?
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-galactonate the same enzyme is responsible for the catabolism of both glucose and galactose Saccharolobus solfataricus pyruvate + D-glyceraldehyde
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-gluconate the same enzyme is responsible for the catabolism of both glucose and galactose Saccharolobus solfataricus pyruvate + D-glyceraldehyde
-
 ?
4.1.2.55 pyruvate + D-glyceraldehyde the enzyme shows a lack of facial selectivity, yielding approximately equal quantities of 2-keto-3-deoxy-D-gluconate and 2-keto-3-deoxy-D-galactonate Saccharolobus solfataricus 2-dehydro-3-deoxy-D-galactonate
-
 r
4.1.2.55 pyruvate + D-glyceraldehyde the enzyme shows a lack of facial selectivity, yielding approximately equal quantities of 2-keto-3-deoxy-D-gluconate and 2-keto-3-deoxy-D-galactonate Saccharolobus solfataricus 2-dehydro-3-deoxy-D-gluconate
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.47 tetramer
-
 Saccharolobus solfataricus
1.1.1.359 tetramer 4 * 41000, SDS-PAGE Saccharolobus solfataricus
1.1.1.359 tetramer 4 * 40849, calculated from sequence Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.47 glucose dehydrogenase
-
 Saccharolobus solfataricus
1.1.1.359 glucose dehydrogenase
-
 Saccharolobus solfataricus
4.1.2.55 2-keto-3-deoxygluconate aldolase
-
 Saccharolobus solfataricus
4.1.2.55 KDG aldolase
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.47 70
-
 assay at Saccharolobus solfataricus
1.1.1.359 70
-
 assay at Saccharolobus solfataricus
4.1.2.55 60
-
 assay at Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.47 37.4
-
 NADP+ with beta-D-galactose, pH 7.5, 70°C Saccharolobus solfataricus
1.1.1.47 47.7
-
 NADP+ with beta-D-glucose, pH 7.5, 70°C Saccharolobus solfataricus
1.1.1.47 61.3
-
 NAD+ with beta-D-galactose, pH 7.5, 70°C Saccharolobus solfataricus
1.1.1.47 74.9
-
 NAD+ with beta-D-glucose, pH 7.5, 70°C Saccharolobus solfataricus
1.1.1.359 37.4
-
 D-galactose pH 7.5, 70°C, cofactor: NADP+ Saccharolobus solfataricus
1.1.1.359 47.7
-
 beta-D-glucose pH 7.5, 70°C, cofactor: NADP+ Saccharolobus solfataricus
1.1.1.359 61.3
-
 D-galactose pH 7.5, 70°C, cofactor: NAD+ Saccharolobus solfataricus
1.1.1.359 74.9
-
 beta-D-glucose pH 7.5, 70°C, cofactor: NAD+ Saccharolobus solfataricus
4.1.2.55 6.8
-
 2-dehydro-3-deoxy-D-galactonate pH 6.0, 60°C Saccharolobus solfataricus
4.1.2.55 28.2
-
 2-dehydro-3-deoxy-D-gluconate pH 6.0, 60°C Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.47 7.5
-
 assay at Saccharolobus solfataricus
1.1.1.359 7.5
-
 assay at Saccharolobus solfataricus
4.1.2.55 6
-
 assay at Saccharolobus solfataricus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.47 additional information dual cofactor specificity due to presence of Asn215, His217, and the GXGXXA motif Saccharolobus solfataricus
1.1.1.47 NAD+
-
 Saccharolobus solfataricus
1.1.1.47 NADP+
-
 Saccharolobus solfataricus
1.1.1.359 NAD+ kcat/Km for beta-D-glucose in the reaction with NAD+ is 1.4fold higher than the kcat/Km value for beta-D-glucose in the reaction with NADP+ Saccharolobus solfataricus
1.1.1.359 NADP+ kcat/Km for beta-D-glucose in the reaction with NADP+ is 1.4fold lower than the kcat/Km value for beta-D-glucose in the reaction with NAD+ Saccharolobus solfataricus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.359 36.7
-
 beta-D-glucose pH 7.5, 70°C, cofactor: NADP+ Saccharolobus solfataricus
1.1.1.359 49.9
-
 beta-D-glucose pH 7.5, 70°C, cofactor: NAD+ Saccharolobus solfataricus
1.1.1.359 85.1
-
 D-galactose pH 7.5, 70°C, cofactor: NADP+ Saccharolobus solfataricus
1.1.1.359 107
-
 D-galactose pH 7.5, 70°C, cofactor: NAD+ Saccharolobus solfataricus
4.1.2.55 0.7
-
 2-dehydro-3-deoxy-D-galactonate pH 6.0, 60°C Saccharolobus solfataricus
4.1.2.55 1.1
-
 2-dehydro-3-deoxy-D-gluconate pH 6.0, 60°C Saccharolobus solfataricus