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Literature summary extracted from

  • Rotsaert, F.A.J.; Hallberg, B.M.; de Vrise, S.; Moenne-Loccoz, P.; Divne, C.; Renganathan, V.; Gold, M.H.
    Biophysical and structural analysis of a novel heme b iron ligation in the flavocytochrome cellobiose dehydrogenase (2003), J. Biol. Chem., 278, 33224-33231.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.18 transformation of Ura- strain Ura11 with linearized pM65H Phanerodontia chrysosporium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.99.18 hanging-drop vapour diffusion method, crystal structure of M65H cytochrome domain determined at 1.9 A resolution Phanerodontia chrysosporium

Protein Variants

EC Number Protein Variants Comment Organism
1.1.99.18 M65H the variant retains the flavin catalytic reactivity, the ability of the mutant to reduce external one-electron acceptors such as cytochrome c is impaired, decrease in the redox midpoint potential of the heme by 210 mV. IN contrast to the wild-type enzyme, the ferric state of the protoheme displays a mixed low spin/high spin state at room temperature and low spion character at 90 K Phanerodontia chrysosporium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.18 0.0008
-
 cytochrome c pH 4.5, reaction with cellobiose, wild-type enzyme Phanerodontia chrysosporium
1.1.99.18 0.0064
-
 2,6-dichlorophenol indophenol pH 4.5, reaction with cellobiose, wild-type enzyme Phanerodontia chrysosporium
1.1.99.18 0.0074
-
 2,6-dichlorophenol indophenol pH 4.5, reaction with cellobiose, mutant enzyme M65H Phanerodontia chrysosporium
1.1.99.18 0.018
-
 cellobiose pH 4.5, electron acceptor: cytochrome c, wild-type enzyme Phanerodontia chrysosporium
1.1.99.18 0.035
-
 cellobiose pH 4.5, electron acceptor: 2,6-dichlorophenol indophenol, mutant enzyme M65H Phanerodontia chrysosporium
1.1.99.18 0.04
-
 cellobiose pH 4.5, electron acceptor: 2,6-dichlorophenol indophenol, wild-type enzyme Phanerodontia chrysosporium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.18 extracellular
-
 Phanerodontia chrysosporium
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.18 Phanerodontia chrysosporium Q01738 variant M65H
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.18
-
 Phanerodontia chrysosporium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.18 cellobiose + 2,6-dichlorophenol indophenol
-
 Phanerodontia chrysosporium cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 cellobiose + cytochrome c
-
 Phanerodontia chrysosporium cellobiono-1,5-lactone + reduced cytochrome c
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.18 0.1
-
 cellobiose pH 4.5, electron acceptor: cytochrome c, mutant enzyme M65H Phanerodontia chrysosporium
1.1.99.18 0.1
-
 cytochrome c pH 4.5, reaction with cellobiose, mutant enzyme M65H Phanerodontia chrysosporium
1.1.99.18 10.5
-
 cellobiose pH 4.5, electron acceptor: cytochrome c, wild-type enzyme Phanerodontia chrysosporium
1.1.99.18 10.8
-
 cytochrome c pH 4.5, reaction with cellobiose, wild-type enzyme Phanerodontia chrysosporium
1.1.99.18 25
-
 2,6-dichlorophenol indophenol pH 4.5, reaction with cellobiose, mutant enzyme M65H Phanerodontia chrysosporium
1.1.99.18 25.7
-
 cellobiose pH 4.5, electron acceptor: 2,6-dichlorophenol indophenol, wild-type enzyme Phanerodontia chrysosporium
1.1.99.18 26
-
 cellobiose pH 4.5, electron acceptor: 2,6-dichlorophenol indophenol, mutant enzyme M65H Phanerodontia chrysosporium
1.1.99.18 27
-
 2,6-dichlorophenol indophenol pH 4.5, reaction with cellobiose, wild-type enzyme Phanerodontia chrysosporium

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.18 flavin flavocytochrome Phanerodontia chrysosporium
1.1.99.18 heme flavocytochrome Phanerodontia chrysosporium