Literature summary extracted from
Reuland, S.N.; Vlasov, A.P.; Krupenko, S.A.
Disruption of a calmodulin central helix-like region of 10-formyltetrahydrofolate dehydrogenase impairs its dehydrogenase activity by uncoupling the functional domains (2003), J. Biol. Chem., 278, 22894-22900.
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.5.1.6 |
D399A |
90% of the dehydrogenase activity of the wild-type enzyme |
Rattus norvegicus |
1.5.1.6 |
E398A |
90% of the dehydrogenase activity of the wild-type enzyme |
Rattus norvegicus |
1.5.1.6 |
F384_V405del |
no dehydrogenase activity |
Rattus norvegicus |
1.5.1.6 |
G397A |
as active as wild-type enzyme |
Rattus norvegicus |
1.5.1.6 |
K394A |
75% of the dehydrogenase activity of the wild-type enzyme |
Rattus norvegicus |
1.5.1.6 |
K394A/L395A/R396A/G397A/E398A/D399A |
no dehydrogenase activity |
Rattus norvegicus |
1.5.1.6 |
K394_D399del |
no dehydrogenase activity |
Rattus norvegicus |
1.5.1.6 |
L395A |
80% of the dehydrogenase activity of the wild-type enzyme |
Rattus norvegicus |
1.5.1.6 |
R396A |
as active as wild-type enzyme |
Rattus norvegicus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.5.1.6 |
Rattus norvegicus |
P28037 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.5.1.6 |
intermediate domain and its mutants |
Rattus norvegicus |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
1.5.1.6 |
-20°C, stable for several months |
Rattus norvegicus |
1.5.1.6 |
4°C, stable for 2 weeks |
Rattus norvegicus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.5.1.6 |
10-formyltetrahydrofolate + NADP+ + H2O |
the enzyme is composed of three domains and possesses three catalytic activities but has only two catalytic centers. The amino-terminal domain (residues 1-310) bears 10-formyltetrahydrofolate hydrolase activity, the carboxyl-terminal domain (residues 420-902) bears an aldehyde dehydrogenase activity, and the full-length FDH produces 10-formyltetrahydrofolate dehydrogenase activity |
Rattus norvegicus |
? |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.5.1.6 |
10-formyltetrahydrofolate dehydrogenase |
- |
Rattus norvegicus |
1.5.1.6 |
FDH |
- |
Rattus norvegicus |