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Literature summary extracted from

  • Fitzpatrick, T.B.; Amrhein, N.; Macheroux, P.
    Characterization of YqjM, an Old Yellow Enzyme homolog from Bacillus subtilis involved in the oxidative stress response (2003), J. Biol. Chem., 278, 19891-19897.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.99.1 0.001
-
 N-ethylmaleimide or below, pH 7.5, 25°C Bacillus subtilis
1.6.99.1 0.109
-
 nitroglycerin pH 7.5, 25°C Bacillus subtilis
1.6.99.1 0.293
-
 cyclohex-2-enone pH 7.5, 25°C Bacillus subtilis
1.6.99.1 0.705
-
 2,4,6-trinitrotoluene pH 7.5, 25°C Bacillus subtilis
1.6.99.1 2.602
-
 trans-hex-2-enal pH 7.5, 25°C Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.99.1 147000
-
 gel fitration Bacillus subtilis
1.6.99.1 374000
-
 4 * 374000, SDS-PAGE, recombinant protein Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.99.1 additional information Bacillus subtilis involved in detoxification and oxidative stress response ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.99.1 Bacillus subtilis
-
 induction by trinitrotoluene and oxidative stress
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.99.1 additional information involved in detoxification and oxidative stress response Bacillus subtilis ?
-
 ?
1.6.99.1 NADPH + H+ + 2,4,6-trinitrotoluene
-
 Bacillus subtilis NADP+ + ?
-
 ?
1.6.99.1 NADPH + H+ + cyclohex-2-enone
-
 Bacillus subtilis NADP+ + ?
-
 ?
1.6.99.1 NADPH + H+ + N-ethylmaleimide
-
 Bacillus subtilis NADP+ + ?
-
 ?
1.6.99.1 NADPH + H+ + nitroglycerin
-
 Bacillus subtilis NADP+ + ?
-
 ?
1.6.99.1 NADPH + H+ + trans-hex-2-enal
-
 Bacillus subtilis NADP+ + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.99.1 tetramer 4 * 374000, SDS-PAGE, recombinant protein Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.99.1 3.19
-
 2,4,6-trinitrotoluene pH 7.5, 25°C Bacillus subtilis
1.6.99.1 3.72
-
 nitroglycerin pH 7.5, 25°C Bacillus subtilis
1.6.99.1 4.38
-
 cyclohex-2-enone pH 7.5, 25°C Bacillus subtilis
1.6.99.1 4.68
-
 trans-hex-2-enal pH 7.5, 25°C Bacillus subtilis
1.6.99.1 8.07
-
 N-ethylmaleimide pH 7.5, 25°C Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.99.1 NADPH
-
 Bacillus subtilis