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Literature summary extracted from

  • Schauer, S.; Chaturvedi, S.; Randau, L.; Moser, J.; Kitabatake, M.; Lorenz, S.; Verkamp, E.; Schubert, W.D.; Nakayashiki, T.; Murai, M.; Wall, K.; Thomann, H.U.; Heinz, D.W.; Inokuchi, H.; Soll, D.; Jahn, D.
    Escherichia coli glutamyl-tRNA reductase. Trapping the thioester intermediate (2002), J. Biol. Chem., 277, 48657-48663.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.70 overexpression in Escherichia coli Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.70 C170S mutant enzyme with esterase activity 95% of the wild-type activity and reductase activity with 90% of the wild-type activity Escherichia coli
1.2.1.70 C50S mutant enzyme with no esterase and reductase activity Escherichia coli
1.2.1.70 C74S mutant enzyme with esterase activity 110% of the wild-type activity and reductase activity with 120% of the wild-type activity Escherichia coli
1.2.1.70 E114K mutant enzyme with no esterase and reductase activity Escherichia coli
1.2.1.70 G106N mutant enzyme with no esterase and reductase activity Escherichia coli
1.2.1.70 G191D mutant enzyme reveals esterase, 105% of the wild-type activity, but no reductase activity Escherichia coli
1.2.1.70 G44C/S105N/A326T mutant enzyme with no esterase and reductase activity Escherichia coli
1.2.1.70 G7D mutant enzyme with no esterase and reductase activity Escherichia coli
1.2.1.70 R314C mutant enzyme with no esterase and reductase activity Escherichia coli
1.2.1.70 S145F mutant enzyme with no esterase and reductase activity Escherichia coli
1.2.1.70 S22L/S164F mutant enzyme with no esterase and reductase activity Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.70 1,10-phenanthroline 5 mM, 25% inhibition Escherichia coli
1.2.1.70 2,2'-dipyridyl 5 mM, 20% inhibition Escherichia coli
1.2.1.70 5,5'-dithiobis(2-nitrobenzoic acid) 1 mM, 80% inhibition Escherichia coli
1.2.1.70 Co2+
-
 Escherichia coli
1.2.1.70 EDTA 10 mM, 55% inhibition Escherichia coli
1.2.1.70 EGTA 10 mM, 35% inhibition Escherichia coli
1.2.1.70 glutamycin 3 mM, 90% inhibition Escherichia coli
1.2.1.70 iodoacetamide 0.1 mM, complete inhibition Escherichia coli
1.2.1.70 Ni2+
-
 Escherichia coli
1.2.1.70 PtCl4 1 mM, 90% inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.70 0.024
-
 L-glutamyl-tRNAGlu pH 8.1, 37°C Escherichia coli
1.2.1.70 0.039
-
 NADPH pH 8.1, 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.70 Ca2+ restores activity after treatment with chelating agents Escherichia coli
1.2.1.70 Mg2+ stimulates, restores activity after treatment with chelating agents Escherichia coli
1.2.1.70 Mn2+ restores activity after treatment with chelating agents Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.70 48000
-
 2 * 48000, homodimer with an extended structure, SDS-PAGE Escherichia coli
1.2.1.70 48448
-
 2 * 48448, homodimer with an extended structure, electrospray ionization mass spectrometry Escherichia coli
1.2.1.70 180000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.70 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.70
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.70 L-glutamyl-tRNAGlu + NADPH + H+ in presence of NADPH, the end product, glutamate-1-semialdehyde is formed. In the absence of NADPH, Escherichia coli GluTR exhibits substrate esterase activity Escherichia coli L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.70 dimer 2 * 48000, homodimer with an extended structure, SDS-PAGE Escherichia coli
1.2.1.70 dimer 2 * 48448, homodimer with an extended structure, electrospray ionization mass spectrometry Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.2.1.70 GluTR
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.70 0.13
-
 L-glutamyl-tRNAGlu pH 8.1, 37°C Escherichia coli
1.2.1.70 0.15
-
 NADPH pH 8.1, 37°C Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.70 NADPH
-
 Escherichia coli