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Literature summary extracted from

  • Hrmova, M.; Imai, T.; Rutten, S.J.; Fairweather, J.K.; Pelosi, L.; Bulone, V.; Driguez, H.; Fincher, G.B.
    Mutated barley (1,3)-beta-D-glucan endohydrolases synthesize crystalline (1,3)-beta-D-glucans (2002), J. Biol. Chem., 277, 30102-30111.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.39 E231A no enzymic activity with laminarin, in presence of sodium formate, recovery of 75% of activitiy compared to wild type, with substrate alpha-laminaribiosyl fluoride, polymerization to insoluble, crystalline (1,3)-beta-D-glucans Hordeum vulgare
3.2.1.39 E231G no enzymic activity with laminarin, in presence of sodium formate, recovery of 75% of activitiy compared to wild type, with substrate alpha-laminaribiosyl fluoride, polymerization to insoluble, crystalline (1,3)-beta-D-glucans Hordeum vulgare
3.2.1.39 E231S no enzymic activity with laminarin, with substrate alpha-laminaribiosyl fluoride, polymerization to insoluble, crystalline (1,3)-beta-D-glucans Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.39 9.7
-
 alpha-laminaribiosyl fluoride pH 6.2, 30°C, mutant E231G Hordeum vulgare
3.2.1.39 19.8
-
 alpha-laminaribiosyl fluoride pH 6.2, 30°C, mutant E231S Hordeum vulgare
3.2.1.39 25.9
-
 alpha-laminaribiosyl fluoride pH 6.2, 30°C, mutant E231A Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.39 33000
-
 x * 33000, SDS-PAGE Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Hordeum vulgare
-
 barley
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.39 220
-
 pH 6.2, 30°C Hordeum vulgare

Storage Stability

EC Number Storage Stability Organism
3.2.1.39 4°C, 50 mM sodium phosphate, pH 7.8, 300 mM NaCl, stable for several weeks Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 alpha-laminaribiosyl fluoride + H2O
-
 Hordeum vulgare ?
-
 ?
3.2.1.39 laminarin + H2O
-
 Hordeum vulgare ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.39 ? x * 33000, SDS-PAGE Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.39 4.8
-
 wild-type Hordeum vulgare

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.39 6 10 broad, mutant E231G Hordeum vulgare