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Literature summary extracted from

  • Park, J.K.; Wang, L.X.; Patel, H.V.; Roseman, S.
    Molecular cloning and characterization of a unique beta-glucosidase from Vibrio cholerae (2002), J. Biol. Chem., 277, 29555-29560.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.74 overexpression in Escherichia coli Vibrio cholerae serotype O1

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.74 EDTA 10% of enzyme inhibition at 20 mM, at 37ºC, pH 6.5 Vibrio cholerae serotype O1
3.2.1.74 EGTA 10% of enzyme inhibition at 20 mM, at 37ºC, pH 6.5 Vibrio cholerae serotype O1
3.2.1.74 KCl 25% of enzyme inhibition at 0.5 mM, at 37ºC, pH 6.5 Vibrio cholerae serotype O1
3.2.1.74 NaCl 25% of enzyme inhibition at 0.5 mM, at 37ºC, pH 6.5 Vibrio cholerae serotype O1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.74 0.45
-
p-nitrophenyl glucoside pH 6.5, at 37ºC Vibrio cholerae serotype O1
3.2.1.74 6.96
-
cellobiose pH 6.5, at 37ºC Vibrio cholerae serotype O1

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.74 cytoplasm
-
Vibrio cholerae serotype O1 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.74 additional information NaCl and KCl gives optimum results at 10 mM, at 37ºC, pH 6.5 Vibrio cholerae serotype O1

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.74 62000
-
calculation from sequence of amino acids Vibrio cholerae serotype O1
3.2.1.74 65000
-
SDS-PAGE Vibrio cholerae serotype O1

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.74 Vibrio cholerae serotype O1
-
-
-
3.2.1.74 Vibrio cholerae serotype O1 E1 Tor N16961
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.74 no modification
-
Vibrio cholerae serotype O1

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.74 centrifugation, streptomycin sulfate precipitation, ammonium sulfate fractionation, DEAE column chromatography, IMAC-Zn2+ chromatography Vibrio cholerae serotype O1

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.74 culture medium
-
Vibrio cholerae serotype O1
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.74 0.323
-
pH 6.5, at 37ºC Vibrio cholerae serotype O1

Storage Stability

EC Number Storage Stability Organism
3.2.1.74 -20ºC or -80ºC, 0.5 mM dithiothreitol Vibrio cholerae serotype O1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.74 cellobiose + H2O no activity with di(N-acetylglucosamine), chitobiose, chitin oligosaccharides, trehalose, lactose, maltose, sucrose, sophorose laminaribiose and gentiobiose Vibrio cholerae serotype O1 2 D-glucose
-
?
3.2.1.74 cellobiose + H2O no activity with di(N-acetylglucosamine), chitobiose, chitin oligosaccharides, trehalose, lactose, maltose, sucrose, sophorose laminaribiose and gentiobiose Vibrio cholerae serotype O1 E1 Tor N16961 2 D-glucose
-
?
3.2.1.74 cellopentaose + H2O
-
Vibrio cholerae serotype O1 glucose + ?
-
?
3.2.1.74 cellopentaose + H2O
-
Vibrio cholerae serotype O1 E1 Tor N16961 glucose + ?
-
?
3.2.1.74 cellotetraose + 3 H2O
-
Vibrio cholerae serotype O1 4 D-glucose
-
?
3.2.1.74 cellotetraose + 3 H2O
-
Vibrio cholerae serotype O1 E1 Tor N16961 4 D-glucose
-
?
3.2.1.74 cellotriose + H2O
-
Vibrio cholerae serotype O1 glucose + cellobiose
-
?
3.2.1.74 cellotriose + H2O
-
Vibrio cholerae serotype O1 E1 Tor N16961 glucose + cellobiose
-
?
3.2.1.74 p-nitrophenyl cellobioside + H2O p-nitrophenyl releasing after a lag period Vibrio cholerae serotype O1 p-nitrophenyl + glucose
-
?
3.2.1.74 p-nitrophenyl cellobioside + H2O p-nitrophenyl releasing after a lag period Vibrio cholerae serotype O1 E1 Tor N16961 p-nitrophenyl + glucose
-
?
3.2.1.74 p-nitrophenyl glucoside + H2O p-nitrophenyl releasing with no lag period, no activity with p-nitrophenyl-?-(N-acetylgalactosamine), p-nitrophenyl-?-glucose-6-phosphate, p-nitrophenyl-(N-acetylglucosamine), p-nitrophenyl-di(N-acetylglucosamine), p-nitrophenyl-tri(N-acetylglucosamine) Vibrio cholerae serotype O1 p-nitrophenyl + glucose
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.74 beta-1,4-glucosidase
-
Vibrio cholerae serotype O1
3.2.1.74 beta-glucosidase
-
Vibrio cholerae serotype O1
3.2.1.74 BglA
-
Vibrio cholerae serotype O1
3.2.1.74 cellobiase
-
Vibrio cholerae serotype O1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.74 37 42
-
Vibrio cholerae serotype O1

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.74 4 37 stable for at least 1 h Vibrio cholerae serotype O1
3.2.1.74 40
-
incubation for 3 h, 40% loss of enzyme activity Vibrio cholerae serotype O1
3.2.1.74 70
-
incubation for 3 h, 100% loss of enzyme activity Vibrio cholerae serotype O1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.74 6 6.5
-
Vibrio cholerae serotype O1