EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.74 | overexpression in Escherichia coli | Vibrio cholerae serotype O1 |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.74 | EDTA | 10% of enzyme inhibition at 20 mM, at 37ºC, pH 6.5 | Vibrio cholerae serotype O1 | |
3.2.1.74 | EGTA | 10% of enzyme inhibition at 20 mM, at 37ºC, pH 6.5 | Vibrio cholerae serotype O1 | |
3.2.1.74 | KCl | 25% of enzyme inhibition at 0.5 mM, at 37ºC, pH 6.5 | Vibrio cholerae serotype O1 | |
3.2.1.74 | NaCl | 25% of enzyme inhibition at 0.5 mM, at 37ºC, pH 6.5 | Vibrio cholerae serotype O1 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.74 | 0.45 | - |
p-nitrophenyl glucoside | pH 6.5, at 37ºC | Vibrio cholerae serotype O1 | |
3.2.1.74 | 6.96 | - |
cellobiose | pH 6.5, at 37ºC | Vibrio cholerae serotype O1 |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.74 | cytoplasm | - |
Vibrio cholerae serotype O1 | 5737 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.74 | additional information | NaCl and KCl gives optimum results at 10 mM, at 37ºC, pH 6.5 | Vibrio cholerae serotype O1 |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.74 | 62000 | - |
calculation from sequence of amino acids | Vibrio cholerae serotype O1 |
3.2.1.74 | 65000 | - |
SDS-PAGE | Vibrio cholerae serotype O1 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.74 | Vibrio cholerae serotype O1 | - |
- |
- |
3.2.1.74 | Vibrio cholerae serotype O1 E1 Tor N16961 | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.2.1.74 | no modification | - |
Vibrio cholerae serotype O1 |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.74 | centrifugation, streptomycin sulfate precipitation, ammonium sulfate fractionation, DEAE column chromatography, IMAC-Zn2+ chromatography | Vibrio cholerae serotype O1 |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.74 | culture medium | - |
Vibrio cholerae serotype O1 | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.74 | 0.323 | - |
pH 6.5, at 37ºC | Vibrio cholerae serotype O1 |
EC Number | Storage Stability | Organism |
---|---|---|
3.2.1.74 | -20ºC or -80ºC, 0.5 mM dithiothreitol | Vibrio cholerae serotype O1 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.74 | cellobiose + H2O | no activity with di(N-acetylglucosamine), chitobiose, chitin oligosaccharides, trehalose, lactose, maltose, sucrose, sophorose laminaribiose and gentiobiose | Vibrio cholerae serotype O1 | 2 D-glucose | - |
? | |
3.2.1.74 | cellobiose + H2O | no activity with di(N-acetylglucosamine), chitobiose, chitin oligosaccharides, trehalose, lactose, maltose, sucrose, sophorose laminaribiose and gentiobiose | Vibrio cholerae serotype O1 E1 Tor N16961 | 2 D-glucose | - |
? | |
3.2.1.74 | cellopentaose + H2O | - |
Vibrio cholerae serotype O1 | glucose + ? | - |
? | |
3.2.1.74 | cellopentaose + H2O | - |
Vibrio cholerae serotype O1 E1 Tor N16961 | glucose + ? | - |
? | |
3.2.1.74 | cellotetraose + 3 H2O | - |
Vibrio cholerae serotype O1 | 4 D-glucose | - |
? | |
3.2.1.74 | cellotetraose + 3 H2O | - |
Vibrio cholerae serotype O1 E1 Tor N16961 | 4 D-glucose | - |
? | |
3.2.1.74 | cellotriose + H2O | - |
Vibrio cholerae serotype O1 | glucose + cellobiose | - |
? | |
3.2.1.74 | cellotriose + H2O | - |
Vibrio cholerae serotype O1 E1 Tor N16961 | glucose + cellobiose | - |
? | |
3.2.1.74 | p-nitrophenyl cellobioside + H2O | p-nitrophenyl releasing after a lag period | Vibrio cholerae serotype O1 | p-nitrophenyl + glucose | - |
? | |
3.2.1.74 | p-nitrophenyl cellobioside + H2O | p-nitrophenyl releasing after a lag period | Vibrio cholerae serotype O1 E1 Tor N16961 | p-nitrophenyl + glucose | - |
? | |
3.2.1.74 | p-nitrophenyl glucoside + H2O | p-nitrophenyl releasing with no lag period, no activity with p-nitrophenyl-?-(N-acetylgalactosamine), p-nitrophenyl-?-glucose-6-phosphate, p-nitrophenyl-(N-acetylglucosamine), p-nitrophenyl-di(N-acetylglucosamine), p-nitrophenyl-tri(N-acetylglucosamine) | Vibrio cholerae serotype O1 | p-nitrophenyl + glucose | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.74 | beta-1,4-glucosidase | - |
Vibrio cholerae serotype O1 |
3.2.1.74 | beta-glucosidase | - |
Vibrio cholerae serotype O1 |
3.2.1.74 | BglA | - |
Vibrio cholerae serotype O1 |
3.2.1.74 | cellobiase | - |
Vibrio cholerae serotype O1 |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.74 | 37 | 42 | - |
Vibrio cholerae serotype O1 |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.74 | 4 | 37 | stable for at least 1 h | Vibrio cholerae serotype O1 |
3.2.1.74 | 40 | - |
incubation for 3 h, 40% loss of enzyme activity | Vibrio cholerae serotype O1 |
3.2.1.74 | 70 | - |
incubation for 3 h, 100% loss of enzyme activity | Vibrio cholerae serotype O1 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.74 | 6 | 6.5 | - |
Vibrio cholerae serotype O1 |