EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
6.3.4.14 | biotin | activates the ATP synthesis reaction with ADP and carbamoyl phosphate as substrates | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.3.4.14 | E276Q | kinetic data, ATP binding residue, reduced maximal velocity, increased Km for ATP | Escherichia coli |
6.3.4.14 | H209A | kinetic data, ATP binding residue, reduced maximal velocity, increased Km for ATP | Escherichia coli |
6.3.4.14 | K116A | kinetic data, ATP binding residue, reduced maximal velocity, increased Km for ATP | Escherichia coli |
6.3.4.14 | K116Q | kinetic data, ATP binding residue, reduced maximal velocity, increased Km for ATP | Escherichia coli |
6.3.4.14 | K159Q | kinetic data, ATP binding residue, reduced maximal velocity, increased Km for ATP | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.14 | additional information | - |
additional information | - |
Escherichia coli | |
6.3.4.14 | 0.19 | - |
ADP | pH 8, 25°C, wild-type enzyme, ATP synthesis reaction with carbamoyl phosphate as cosubstrate | Escherichia coli | |
6.3.4.14 | 4.8 | - |
Carbamoyl phosphate | pH 8, 25°C, wild-type enzyme, ATP synthesis reaction with ADP as cosubstrate | Escherichia coli | |
6.3.4.14 | 125 | - |
biotin | pH 8, 25°C, K159Q mutant | Escherichia coli | |
6.3.4.14 | 134 | - |
biotin | wild-type enzyme | Escherichia coli | |
6.3.4.14 | 137 | - |
biotin | pH 8, 25°C, E276Q mutant | Escherichia coli | |
6.3.4.14 | 147 | - |
biotin | pH 8, 25°C, K116A mutant | Escherichia coli | |
6.3.4.14 | 1234 | - |
biotin | pH 8, 25°C, H209A mutant | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.3.4.14 | Mg2+ | requires two equivalents of magnesium for activity, one is complexed to ATP, the role of the other is unknown | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.3.4.14 | 50000 | - |
2 * 50000 | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.4.14 | ATP + biotin-carboxyl-carrier protein + HCO3- | Escherichia coli | long-chain fatty acid synthesis, in vivo biotin is linked to the biotin-carboxyl-carrier protein through an amide bond to a specific lysine residue | ADP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.4.14 | Escherichia coli | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
6.3.4.14 | ATP + [biotin carboxyl-carrier protein]-biotin-N6-L-lysine + hydrogencarbonate- = ADP + phosphate + [biotin carboxyl-carrier protein]-carboxybiotin-N6-L-lysine | catalytic mechanism | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.4.14 | ADP + carbamoyl phosphate | biotin carboxylase catalyzes an ATP synthesis reaction, in which a phosphate group is transferred from carbamoyl phosphate to ADP forming ATP and carbamate | Escherichia coli | ATP + carbamate | carbamate rapidly decomposes into carbon dioxide and ammonia | ? | |
6.3.4.14 | ATP + biotin + HCO3- | also uses free biotin as substrate | Escherichia coli | ADP + phosphate + carboxybiotin | - |
? | |
6.3.4.14 | ATP + biotin-carboxyl-carrier protein + HCO3- | long-chain fatty acid synthesis, in vivo biotin is linked to the biotin-carboxyl-carrier protein through an amide bond to a specific lysine residue | Escherichia coli | ADP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
? | |
6.3.4.14 | ATP + biotin-carboxyl-carrier protein + HCO3- | the biotin carboxylase component of acetyl-CoA carboxylase catalyzes the ATP-dependent carboxylation of biotin using bicarbonate as the carboxylate source, Lys-116, Lys-159, His-209 and Glu-276 are involved in ATP binding and in catalysis orienting ATP in a conformation that allows for optimal catalysis, mechanism | Escherichia coli | ADP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
r | |
6.3.4.14 | additional information | in the absence of biotin biotin carboxylase catalyzes a bicarbonate-dependent ATP hydrolysis at a slow rate | Escherichia coli | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.3.4.14 | homodimer | 2 * 50000 | Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.3.4.14 | More | ATP-grasp superfamily | Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.3.4.14 | 25 | - |
assay at | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.14 | additional information | - |
additional information | - |
Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.3.4.14 | 8 | - |
assay at | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.3.4.14 | ATP | Lys-116, Lys-159, His-209 and Glu-276 are involved in ATP binding | Escherichia coli |