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Literature summary extracted from

  • Moser, J.; Lorenz, S.; Hubschwerlen, C.; Rompf, A.; Jahn, D.
    Methanopyrus kandleri glutamyl-tRNA reductase (1999), J. Biol. Chem., 274, 30679-30685.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.70 overexpression in Escherichia coli Methanopyrus kandleri

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.70 C393S 95% of the GluTR reductase activity compared to wild-type enzyme, 100% of the GluTR esterase activity compared to wild-type enzyme Methanopyrus kandleri
1.2.1.70 C42S no GluTR reductase and GluTR esterase activity Methanopyrus kandleri
1.2.1.70 C48S 90% of the GluTR reductase activity compared to wild-type enzyme, 95% of the GluTR esterase activity compared to wild-type enzyme Methanopyrus kandleri
1.2.1.70 C6S 130% of the GluTR reductase activity compared to wild-type enzyme, 120% of the GluTR esterase activity compared to wild-type enzyme Methanopyrus kandleri
1.2.1.70 C90S 85% of the GluTR reductase activity compared to wild-type enzyme, 105% of the GluTR esterase activity compared to wild-type enzyme Methanopyrus kandleri
1.2.1.70 H84A no GluTR reductase activity, 5% of the GluTR esterase activity compared to wild-type enzyme Methanopyrus kandleri
1.2.1.70 H84N 30% of the GluTR reductase activity compared to wild-type enzyme, 15% of the GluTR esterase activity compared to wild-type enzyme Methanopyrus kandleri

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.70 5,5'-dithiobis(2-nitrobenzoic acid) 1.0 mM, 90% inhibition Methanopyrus kandleri
1.2.1.70 glutamate-1-semialdehyde 1.0 mM, 50% inhibition Methanopyrus kandleri
1.2.1.70 glutamycin 2.5 mM, 75% inhibition Methanopyrus kandleri
1.2.1.70 heme 0.007 mM, 70% inhibition Methanopyrus kandleri
1.2.1.70 iodoacetamide 0.01 mM, 30% inhibition, 0.1 mM, complete inhibition Methanopyrus kandleri
1.2.1.70 N-tosyl-L-phenylalaninechloromethyl ketone 0.1 mM, 90% inhibition, 1.0 mM, complete inhibition Methanopyrus kandleri
1.2.1.70 PbCl2 0.1 mM, 60% inhibition, 1.0 mM, complete inhibition Methanopyrus kandleri
1.2.1.70 PtCl4 0.1 mM, 55% inhibition, 1.0 mM, 90% inhibition Methanopyrus kandleri
1.2.1.70 ZnCl2 0.2 mM, 45% inhibition, 5.0 mM, 90% inhibition Methanopyrus kandleri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.70 45436
-
x * 45436, electrospray ionization mass spectrometry Methanopyrus kandleri
1.2.1.70 190000
-
gel filtration Methanopyrus kandleri

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.70 Methanopyrus kandleri Q9UXR8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.70
-
Methanopyrus kandleri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.70 L-glutamyl-tRNAGlu + NADPH + H+ in absence of NADPH, an esterase activity of GluTR hydrolyzes the highly reactive thioester of tRNAGlu to release glutamate Methanopyrus kandleri L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
?

Subunits

EC Number Subunits Comment Organism
1.2.1.70 ? x * 45436, electrospray ionization mass spectrometry Methanopyrus kandleri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.70 90
-
-
Methanopyrus kandleri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.70 8.1
-
-
Methanopyrus kandleri

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.70 additional information the enzyme does not possess a chromophoric prosthetic group Methanopyrus kandleri
1.2.1.70 NADPH
-
Methanopyrus kandleri

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.2.1.70 Methanopyrus kandleri isoelectric focusing
-
6