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Literature summary extracted from

  • Nakai, C.; Thomas, J.A.
    Properties of a phosphoprotein phosphatase from bovine heart with activity on glycogen synthase, phosphorylase, and histone (1974), J. Biol. Chem., 249, 6459-6467.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.16 ADP 1.4 mM, 30°C, pH 7.5, 104% relative activity with histone as substrate Bos taurus
3.1.3.16 AMP 1.4 mM, 30°C, pH 7.5, 102% relative activity with histone as substrate Bos taurus
3.1.3.16 ATP 1.4 mM, 30°C, pH 7.5, 107% relative activity with histone as substrate Bos taurus
3.1.3.16 bovine serum albumin 10 mg/ml, 30°C, pH 7.5, 112% relative activity with histone as substrate Bos taurus
3.1.3.16 CaCl2 14 mM, 30°C, pH 7.5, relative activity 126% with glycogen synthase D as substrate and 232% with histone as substrate compared to no addition Bos taurus
3.1.3.16 cAMP 1 mM, 30°C, pH 7.5, 115% relative activity with glycogen synthase D as substrate Bos taurus
3.1.3.16 D-glucosamine 6-phosphate 1.4 mM, 30°C, pH7.5, relative activity 109% with glycogen synthase D as substrate, 117% with histone as substrate, and 102% with phosphorylase a as substrate compared to no addition Bos taurus
3.1.3.16 D-glucose 1.4 mM, 30°C, pH7.5, relative activity 109% with glycogen synthase D as substrate, 101% with histone as substrate, and 106% with phosphorylase a as substrate compared to no addition Bos taurus
3.1.3.16 D-glucose 6-phosphate 1.4 mM, 30°C, pH 7.5, 109% relative activity with phosphorylase a as substrate Bos taurus
3.1.3.16 EDTA 14 mM, 30°C, pH 7.5, 175% relative activity with histone as substrate Bos taurus
3.1.3.16 EGTA 14 mM, 30°C, pH 7.5, 127% relative activity with histone as substrate Bos taurus
3.1.3.16 gelatin 1 mg/ml, 30°C, pH 7.5, 128% relative activity with glycogen synthase D as substrate, with 10 mg/ml 110% relative activity Bos taurus
3.1.3.16 MgCl2 14 mM, 30°C, pH 7.5, relative activity 256% with glycogen synthase D as substrate and 238% with histone as substrate compared to no addition Bos taurus
3.1.3.16 MnCl2 14 mM, 30°C, pH 7.5, relative activity 361% with glycogen synthase D as substrate and 280% with histone as substrate compared to no addition Bos taurus
3.1.3.16 additional information study of effects of D-glucose 6-phosphate on activating compounds Bos taurus
3.1.3.16 additional information study of effects of MgCl2 in presence of AMP, ADP, ATP, UMP, UDP, UTP, or citrate Bos taurus
3.1.3.16 Na2SO4 14 mM, 30°C, pH 7.5, relative activity 152% with histone as substrate compared to no addition Bos taurus
3.1.3.16 NaCl 14 mM, 30°C, pH 7.5, relative activity 102% with glycogen synthase D as substrate and 108% with histone as substrate compared to no addition Bos taurus
3.1.3.16 rabbit liver glycogen 1 mg/ml, 30°C, pH 7.5, 119% relative activity with histone as substrate Bos taurus
3.1.3.16 UMP 1.4 mM, 30°C, pH 7.5, 102% relative activity with histone as substrate Bos taurus
3.1.3.16 UTP 1.4 mM, 30°C, pH 7.5, 108% relative activity with histone as substrate Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.16 ADP 1.4 mM, 30°C, pH 7.5, 55% remaining activity with glycogen synthase D as substrate and 12% with phosphorylase a Bos taurus
3.1.3.16 AMP 1.4 mM, 30°C, pH 7.5, 55% remaining activity with glycogen synthase D as substrate and 10% with phosphorylase a Bos taurus
3.1.3.16 ATP 1.4 mM, 30°C, pH 7.5, 47% remaining activity with glycogen synthase D as substrate and 15% with phosphorylase a Bos taurus
3.1.3.16 bovine serum albumin 1 mg/ml 30°C, pH 7.5, 95% remaining activity with glycogen synthase D and 92% with phosphorylase, in presence of 10 mg/ml 79% remaining activity with phosphorylase a as substrate Bos taurus
3.1.3.16 CaCl2 14 mM, 30°C, pH 7.5, with phosphorylase a as substrate, 50% remaining activity Bos taurus
3.1.3.16 cAMP 1 mM, 30°C, pH 7.5, 83% remaining activity with histone as substrate and 83% with phosphorylase a Bos taurus
3.1.3.16 citrate 1.4 mM, 30°C, pH 7.5, 68% remaining activity with glycogen synthase D as substrate Bos taurus
3.1.3.16 EDTA 14 mM, 30°C, pH 7.5, 23% remaining activity with glycogen synthase D as substrate and 35% with phosphorylase a Bos taurus
3.1.3.16 EGTA 14 mM, 30°C, pH 7.5, 55% remaining activity with glycogen synthase D as substrate and 83% with phosphorylase a Bos taurus
3.1.3.16 gelatin 1 mg/ml, 30°C, pH 7.5, 92% remaining activity with histone as substrate, and 31% withe phosphorylase a, in presence of 10 mg/ml 10% remaining activity with phohsphorylase a Bos taurus
3.1.3.16 histone competitive when glycogen synthase D is the substrate, increases the Km-value for glycogen synthase D 4fold, but shows mixed type inhibition when phosphorylase a acts as substrate with an 1.4fold increase of Km Bos taurus
3.1.3.16 MgCl2 14 mM, 30°C, pH 7.5, with phosphorylase a as substrate, 60% remaining activity Bos taurus
3.1.3.16 MnCl2 14 mM, 30°C, pH 7.5, with phosphorylase a as substrate, 51% remaining activity Bos taurus
3.1.3.16 additional information study of effects of D-glucose 6-phosphate on inhibitory compounds; study of effects of MgCl2 in presence of AMP, ADP, ATP, UMP, UDP, UTP, or citrate Bos taurus
3.1.3.16 Na2HPO4 14 mM, 30°C, pH 7.5, no activity with glycogen synthase D as substrate, remaining activity 79% with histone as substrate, and 64% with phosphorylase a as substrate Bos taurus
3.1.3.16 Na2P2O7 14 mM, 30°C, pH 7.5, remaining activity 6% with glycogen synthase D as substrate, 15% with histone as substrate, and 8% with phosphorylase a as substrate Bos taurus
3.1.3.16 Na2SO4 14 mM, 30°C, pH 7.5, remaining activity 53% with glycogen synthase D as substrate and 32% with phosphorylase a as substrate Bos taurus
3.1.3.16 NaCl 14 mM, 30°C, pH 7.5, with phosphorylase a as substrate, 83% remaining activity Bos taurus
3.1.3.16 NaF 14 mM, 30°C, pH 7.5, remaining activity 31% with glycogen synthase D as substrate, 67% with histone as substrate, and 37% with phosphorylase a as substrate Bos taurus
3.1.3.16 NaHCO3 14 mM, 30°C, pH 7.5, remaining activity 49% with glycogen synthase D as substrate, 79% with histone as substrate, and 64% with phosphorylase a as substrate Bos taurus
3.1.3.16 NaNO3 14 mM, 30°C, pH 7.5, remaining activity 70% with glycogen synthase D as substrate, 83% with histone as substrate, and 78% with phosphorylase a as substrate Bos taurus
3.1.3.16 phosphorylase a mixed type inhibitor with glycogen synthase d as substrate, increases Km 2fold with only small decearse in vmax Bos taurus
3.1.3.16 rabbit liver glycogen 1 mg/ml 30°C, pH 7.5, 88% remaining activity with glycogen synthase D and 37% remaining activity with 10 mg/ml inhibitor Bos taurus
3.1.3.16 UDP 1.4 mM, 30°C, pH 7.5, 52% remaining activity with glycogen synthase D as substrate and 20% with phosphorylase a Bos taurus
3.1.3.16 UMP 1.4 mM, 30°C, pH 7.5, 58% remaining activity with glycogen synthase D as substrate and 16% with phosphorylase a Bos taurus
3.1.3.16 UTP 1.4 mM, 30°C, pH 7.5, 44% remaining activity with glycogen synthase D as substrate and 21% with phosphorylase a Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.16 additional information
-
 additional information kinetics with glycogen synthase D, phosphorylase a, and histone in presence of no, 10, 35, and 100 mM MgCl2, expressed as concentrations of [32P] phosphate Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.16 phosphoprotein + H2O Bos taurus
-
 protein + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.16 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.16 150fold purified, ratio of activities with glycogen synthase D, phosphorylase a, and histone as substrates are unchanged during purification, final enzyme preparation is also active on phosphorylase kinase and casein, no activity on p-nitrophenyl phosphate, when assayed at pH 7.5 or at pH 10.5 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.16 heart
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.16 glycogen synthase D + H2O
-
 Bos taurus glycogen synthase I + phosphate
-
 r
3.1.3.16 additional information no activity on p-nitrophenyl phosphate, when assayed at pH 7.5 or at pH 10.5 Bos taurus ?
-
 ?
3.1.3.16 phosphocasein + H2O
-
 Bos taurus casein + phosphate
-
 ?
3.1.3.16 phosphoprotein + H2O
-
 Bos taurus protein + phosphate
-
 ?
3.1.3.16 phosphorylase a + H2O
-
 Bos taurus phosphorylase b + phosphate
-
 r
3.1.3.16 phosphorylated histone + H2O
-
 Bos taurus histone + phosphate
-
 ?
3.1.3.16 phosphorylated phosphorylase kinase + H2O
-
 Bos taurus phosphorylase kinase + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.16 general phosphoprotein phosphatase
-
 Bos taurus