Literature summary extracted from
Bhattacharjee, H.; Rosen, B.P.
Structure-function analysis of the ArsA ATPase: contribution of histidine residues (2001), J. Bioenerg. Biomembr., 33, 459-468.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
7.3.2.7 |
expression in Escherichia coli JM109 with a His-tag |
Escherichia coli |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
7.3.2.7 |
H138A |
cells with this protein were resistant to arsenite and antimonite like wild-type |
Escherichia coli |
7.3.2.7 |
H148A |
cells with this protein were resistant to arsenite and antimonite like wild-type, 3-fold lowering of the antimonite stimulated ATPase activity compared to wild-type enzyme |
Escherichia coli |
7.3.2.7 |
H219A |
cells with this protein were resistant to arsenite and antimonite like wild-type |
Escherichia coli |
7.3.2.7 |
H327A |
cells with this protein were resistant to arsenite and antimonite like wild-type |
Escherichia coli |
7.3.2.7 |
H359A |
cells with this protein were resistant to arsenite and antimonite like wild-type |
Escherichia coli |
7.3.2.7 |
H368A |
cells with this protein were resistant to arsenite and antimonite like wild-type, 3-fold lowering of the antimonite stimulated ATPase activity compared to wild-type enzyme |
Escherichia coli |
7.3.2.7 |
H388A |
cells with this protein were resistant to arsenite and antimonite like wild-type |
Escherichia coli |
7.3.2.7 |
H397A |
cells with this protein were resistant to arsenite and antimonite like wild-type |
Escherichia coli |
7.3.2.7 |
H453A |
cells with this protein were resistant to arsenite and antimonite like wild-type, 3-fold lowering of the antimonite stimulated ATPase activity compared to wild-type enzyme |
Escherichia coli |
7.3.2.7 |
H465A |
cells with this protein were resistant to arsenite and antimonite like wild-type |
Escherichia coli |
7.3.2.7 |
H477A |
cells with this protein were resistant to arsenite and antimonite like wild-type |
Escherichia coli |
7.3.2.7 |
H520A |
cells with this protein were resistant to arsenite and antimonite like wild-type |
Escherichia coli |
7.3.2.7 |
H558A |
cells with this protein were resistant to arsenite and antimonite like wild-type, 50% lowering of the antimonite stimulated ATPase activity compared to wild-type enzyme |
Escherichia coli |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
7.3.2.7 |
diethyl dicarbonate |
inhibition could be reversed upon subsequent incubation with hydroxylamine |
Escherichia coli |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
7.3.2.7 |
Escherichia coli |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
7.3.2.7 |
- |
Escherichia coli |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
7.3.2.7 |
ArsA ATPase |
- |
Escherichia coli |