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Literature summary extracted from

  • Kim, K.J.; Yang, Y.J.; Kim, J.G.
    Purification and characterization of chitinase from Streptomyces sp. M-20 (2003), J. Biochem. Mol. Biol., 36, 185-189.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.14 2-mercaptoethanol
-
 Streptomyces sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 Ag+ strong inhibition Streptomyces sp.
3.2.1.14 Co2+ slight inhibition Streptomyces sp.
3.2.1.14 Fe2+ slight inhibition Streptomyces sp.
3.2.1.14 Fe3+ slight inhibition Streptomyces sp.
3.2.1.14 Hg+ complete inhibition Streptomyces sp.
3.2.1.14 Hg2+ complete inhibition Streptomyces sp.
3.2.1.14 Mn2+ slight inhibition Streptomyces sp.
3.2.1.14 p-chloromercuribenzoate complete inhibition Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 20000
-
 x * 20000, SDS-PAGE Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Streptomyces sp.
-
 M-20
-
3.2.1.14 Streptomyces sp. M-20
-
 M-20
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14
-
 Streptomyces sp.

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.2.1.14 refolding after SDS-PAGE by the removal of SDS with purified Triton X-100 Streptomyces sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 137
-
 after Sephadex G-100 purification step Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O colloidal Streptomyces sp. ?
-
 ?
3.2.1.14 chitin + H2O colloidal Streptomyces sp. M-20 ?
-
 ?
3.2.1.14 N,N',N''-triacetylchitotriose + 2 H2O
-
 Streptomyces sp. 3 N-acetyl-D-glucosamine
-
 ?
3.2.1.14 N,N',N''-triacetylchitotriose + 2 H2O
-
 Streptomyces sp. M-20 3 N-acetyl-D-glucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 20000, SDS-PAGE Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 30
-
-
 Streptomyces sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 40
-
 decrease in activity Streptomyces sp.
3.2.1.14 60
-
 loss of activity Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 5
-
-
 Streptomyces sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 4 8
-
 Streptomyces sp.