BRENDA - Enzyme Database

Cloning and expression in Escherichia coli of the D-aspartate oxidase gene from the yeast Cryptococcus humicola and characterization of the recombinant enzyme

Takahashi, S.; Takahashi, T.; Kera, Y.; Matsunaga, R.; Shibuya, H.; Yamada, R.H.; J. Biochem. 135, 533-540 (2004)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.4.3.1
expression in Escherichia coli
Vanrija humicola
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.3.1
D-malate
33% inhibition
Vanrija humicola
1.4.3.1
malonate
60% inhibition
Vanrija humicola
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.3.1
2.9
-
D-Aspartate
37°C, pH 7.5
Vanrija humicola
1.4.3.1
62.6
-
D-glutamate
37°C, pH 7.5
Vanrija humicola
1.4.3.1
85.3
-
N-Methyl-D-aspartate
37°C, pH 7.5
Vanrija humicola
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.4.3.1
39459
-
4 * 39459, deduced from nucleotide sequence
Vanrija humicola
1.4.3.1
40000
-
4 * 40000, SDS-PAGE
Vanrija humicola
1.4.3.1
164000
-
gel filtration
Vanrija humicola
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.4.3.1
Vanrija humicola
Q75WF1
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.4.3.1
recombinant chDDO, ammonium sulfate, Butyl-Sepharose, SP-Sepharose
Vanrija humicola
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.4.3.1
123
-
-
Vanrija humicola
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.3.1
D-aspartate + H2O + O2
-
655928
Vanrija humicola
oxaloacetate + NH3 + H2O2
-
-
-
?
1.4.3.1
D-glutamate + H2O + O2
-
655928
Vanrija humicola
2-oxoglutarate + NH3 + H2O2
-
-
-
?
1.4.3.1
N-methyl-D-aspartate + H2O + O2
-
655928
Vanrija humicola
oxaloacetate + methylamine + H2O2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.4.3.1
tetramer
4 * 39459, deduced from nucleotide sequence; 4 * 40000, SDS-PAGE
Vanrija humicola
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.4.3.1
37
-
-
Vanrija humicola
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.4.3.1
40
-
little loss of activity
Vanrija humicola
1.4.3.1
60
-
complete loss of activity
Vanrija humicola
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.4.3.1
2
-
D-glutamate
37°C, pH 7.5
Vanrija humicola
1.4.3.1
38.1
-
N-Methyl-D-aspartate
37°C, pH 7.5
Vanrija humicola
1.4.3.1
82.6
-
D-Aspartate
37°C, pH 7.5
Vanrija humicola
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.4.3.1
7.5
-
-
Vanrija humicola
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.4.3.1
expression in Escherichia coli
Vanrija humicola
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.3.1
D-malate
33% inhibition
Vanrija humicola
1.4.3.1
malonate
60% inhibition
Vanrija humicola
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.3.1
2.9
-
D-Aspartate
37°C, pH 7.5
Vanrija humicola
1.4.3.1
62.6
-
D-glutamate
37°C, pH 7.5
Vanrija humicola
1.4.3.1
85.3
-
N-Methyl-D-aspartate
37°C, pH 7.5
Vanrija humicola
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.4.3.1
39459
-
4 * 39459, deduced from nucleotide sequence
Vanrija humicola
1.4.3.1
40000
-
4 * 40000, SDS-PAGE
Vanrija humicola
1.4.3.1
164000
-
gel filtration
Vanrija humicola
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.4.3.1
recombinant chDDO, ammonium sulfate, Butyl-Sepharose, SP-Sepharose
Vanrija humicola
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.4.3.1
123
-
-
Vanrija humicola
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.3.1
D-aspartate + H2O + O2
-
655928
Vanrija humicola
oxaloacetate + NH3 + H2O2
-
-
-
?
1.4.3.1
D-glutamate + H2O + O2
-
655928
Vanrija humicola
2-oxoglutarate + NH3 + H2O2
-
-
-
?
1.4.3.1
N-methyl-D-aspartate + H2O + O2
-
655928
Vanrija humicola
oxaloacetate + methylamine + H2O2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.4.3.1
tetramer
4 * 39459, deduced from nucleotide sequence; 4 * 40000, SDS-PAGE
Vanrija humicola
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.4.3.1
37
-
-
Vanrija humicola
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.4.3.1
40
-
little loss of activity
Vanrija humicola
1.4.3.1
60
-
complete loss of activity
Vanrija humicola
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.4.3.1
2
-
D-glutamate
37°C, pH 7.5
Vanrija humicola
1.4.3.1
38.1
-
N-Methyl-D-aspartate
37°C, pH 7.5
Vanrija humicola
1.4.3.1
82.6
-
D-Aspartate
37°C, pH 7.5
Vanrija humicola
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.4.3.1
7.5
-
-
Vanrija humicola