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Literature summary extracted from

  • Takahashi, S.; Takahashi, T.; Kera, Y.; Matsunaga, R.; Shibuya, H.; Yamada, R.H.
    Cloning and expression in Escherichia coli of the D-aspartate oxidase gene from the yeast Cryptococcus humicola and characterization of the recombinant enzyme (2004), J. Biochem., 135, 533-540.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.1 expression in Escherichia coli Vanrija humicola

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.1 D-malate 33% inhibition Vanrija humicola
1.4.3.1 malonate 60% inhibition Vanrija humicola

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.1 2.9
-
 D-Aspartate 37°C, pH 7.5 Vanrija humicola
1.4.3.1 62.6
-
 D-glutamate 37°C, pH 7.5 Vanrija humicola
1.4.3.1 85.3
-
 N-Methyl-D-aspartate 37°C, pH 7.5 Vanrija humicola

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.1 39459
-
 4 * 39459, deduced from nucleotide sequence Vanrija humicola
1.4.3.1 40000
-
 4 * 40000, SDS-PAGE Vanrija humicola
1.4.3.1 164000
-
 gel filtration Vanrija humicola

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.1 Vanrija humicola Q75WF1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.1 recombinant chDDO, ammonium sulfate, Butyl-Sepharose, SP-Sepharose Vanrija humicola

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.1 123
-
-
 Vanrija humicola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.1 D-aspartate + H2O + O2
-
 Vanrija humicola oxaloacetate + NH3 + H2O2
-
 ?
1.4.3.1 D-glutamate + H2O + O2
-
 Vanrija humicola 2-oxoglutarate + NH3 + H2O2
-
 ?
1.4.3.1 N-methyl-D-aspartate + H2O + O2
-
 Vanrija humicola oxaloacetate + methylamine + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.1 tetramer 4 * 40000, SDS-PAGE Vanrija humicola
1.4.3.1 tetramer 4 * 39459, deduced from nucleotide sequence Vanrija humicola

Synonyms

EC Number Synonyms Comment Organism
1.4.3.1 ChDDO
-
 Vanrija humicola

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.1 37
-
-
 Vanrija humicola

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.1 40
-
 little loss of activity Vanrija humicola
1.4.3.1 60
-
 complete loss of activity Vanrija humicola

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.1 2
-
 D-glutamate 37°C, pH 7.5 Vanrija humicola
1.4.3.1 38.1
-
 N-Methyl-D-aspartate 37°C, pH 7.5 Vanrija humicola
1.4.3.1 82.6
-
 D-Aspartate 37°C, pH 7.5 Vanrija humicola

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.1 7.5
-
-
 Vanrija humicola