Literature summary extracted from
Ohtani, N.; Haruki, M.; Muroya, A.; Morikawa, M.; Kanaya, S.
Characterization of ribonuclease HII from Escherichia coli overproduced in a soluble form (2000), J. Biochem., 127, 895-899.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.1.26.4 |
gene rnhB, overexpression of the enzyme in an insoluble form in different strains of Escherichia coli, overexpression of soluble enzyme with a self-cleavable intein-tag |
Escherichia coli |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.1.26.4 |
KCl |
activates by 50% at 50 mM, inhibits by 90% at 200 mM |
Escherichia coli |
|
3.1.26.4 |
NaCl |
activates by 50% at 50 mM, inhibits by 90% at 200 mM |
Escherichia coli |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
3.1.26.4 |
0.00026 |
- |
RNA*DNA hybrid |
pH 8.5, 30°C, recombinant soluble non-tagged enzyme |
Escherichia coli |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.1.26.4 |
KCl |
activates at 50 mM, inhibits at 200 mM |
Escherichia coli |
|
3.1.26.4 |
Mg2+ |
absolutely dependent on for activity, can be substituted by Mn2+ |
Escherichia coli |
|
3.1.26.4 |
Mn2+ |
absolutely dependent on for activity, can be substituted by Mg2+ |
Escherichia coli |
|
3.1.26.4 |
additional information |
no activity in absence of Mg2+ or Mn2+, and in presence of 10 mM of Ba2+, Ca2+, Co2+, Zn2+, Cu2+, Fe2+, or Sr2+ |
Escherichia coli |
|
3.1.26.4 |
NaCl |
activates at 50 mM, inhibits at 200 mM |
Escherichia coli |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.1.26.4 |
22000 |
- |
recombinant soluble non-tagged enzyme, gel filtration |
Escherichia coli |
3.1.26.4 |
23000 |
- |
1 * 23000, recombinant soluble non-tagged enzyme, SDS-PAGE |
Escherichia coli |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.26.4 |
Escherichia coli |
- |
type II enzyme, gene rnhB |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.1.26.4 |
recombinant insoluble enzyme in a urea-denatured form, recombinant soluble intein-tagged enzyme |
Escherichia coli |
Renatured (Commentary)
EC Number |
Renatured (Comment) |
Organism |
---|
3.1.26.4 |
refolding from purified urea-denatured form with low yield |
Escherichia coli |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
3.1.26.4 |
additional information |
- |
the activity of the recombinant soluble enzyme is slightly higher than that of the recombinant refolded enzyme |
Escherichia coli |
3.1.26.4 |
0.75 |
- |
recombinant soluble nontagged enzyme |
Escherichia coli |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.1.26.4 |
RNA*DNA hybrid + H2O |
- |
Escherichia coli |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.1.26.4 |
monomer |
1 * 23000, recombinant soluble non-tagged enzyme, SDS-PAGE |
Escherichia coli |
3.1.26.4 |
More |
helical content of the enzyme structure is 23% |
Escherichia coli |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.1.26.4 |
ribonuclease HII |
- |
Escherichia coli |
3.1.26.4 |
RNase H |
- |
Escherichia coli |
3.1.26.4 |
RNase HII |
- |
Escherichia coli |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.1.26.4 |
30 |
- |
assay at |
Escherichia coli |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.1.26.4 |
9.8 |
- |
above |
Escherichia coli |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
3.1.26.4 |
7 |
9.8 |
enzyme is inactive below pH 7.0, activity increases with increasing pH |
Escherichia coli |