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Literature summary extracted from

  • Ohtani, N.; Haruki, M.; Muroya, A.; Morikawa, M.; Kanaya, S.
    Characterization of ribonuclease HII from Escherichia coli overproduced in a soluble form (2000), J. Biochem., 127, 895-899.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.26.4 gene rnhB, overexpression of the enzyme in an insoluble form in different strains of Escherichia coli, overexpression of soluble enzyme with a self-cleavable intein-tag Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.26.4 KCl activates by 50% at 50 mM, inhibits by 90% at 200 mM Escherichia coli
3.1.26.4 NaCl activates by 50% at 50 mM, inhibits by 90% at 200 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.26.4 0.00026
-
RNA*DNA hybrid pH 8.5, 30°C, recombinant soluble non-tagged enzyme Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.26.4 KCl activates at 50 mM, inhibits at 200 mM Escherichia coli
3.1.26.4 Mg2+ absolutely dependent on for activity, can be substituted by Mn2+ Escherichia coli
3.1.26.4 Mn2+ absolutely dependent on for activity, can be substituted by Mg2+ Escherichia coli
3.1.26.4 additional information no activity in absence of Mg2+ or Mn2+, and in presence of 10 mM of Ba2+, Ca2+, Co2+, Zn2+, Cu2+, Fe2+, or Sr2+ Escherichia coli
3.1.26.4 NaCl activates at 50 mM, inhibits at 200 mM Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.26.4 22000
-
recombinant soluble non-tagged enzyme, gel filtration Escherichia coli
3.1.26.4 23000
-
1 * 23000, recombinant soluble non-tagged enzyme, SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.4 Escherichia coli
-
type II enzyme, gene rnhB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.26.4 recombinant insoluble enzyme in a urea-denatured form, recombinant soluble intein-tagged enzyme Escherichia coli

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.1.26.4 refolding from purified urea-denatured form with low yield Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.26.4 additional information
-
the activity of the recombinant soluble enzyme is slightly higher than that of the recombinant refolded enzyme Escherichia coli
3.1.26.4 0.75
-
recombinant soluble nontagged enzyme Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.4 RNA*DNA hybrid + H2O
-
Escherichia coli ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.26.4 monomer 1 * 23000, recombinant soluble non-tagged enzyme, SDS-PAGE Escherichia coli
3.1.26.4 More helical content of the enzyme structure is 23% Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.1.26.4 ribonuclease HII
-
Escherichia coli
3.1.26.4 RNase H
-
Escherichia coli
3.1.26.4 RNase HII
-
Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.26.4 30
-
assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.26.4 9.8
-
above Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.26.4 7 9.8 enzyme is inactive below pH 7.0, activity increases with increasing pH Escherichia coli