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Literature summary extracted from

  • Marques De Souza, C.G.; Peralta, R.M.
    Purification and characterization of the main laccase produced by the white-rot fungus Pleurotus pulmonarius on wheat bran solid state medium (2003), J. Basic Microbiol., 43, 278-286.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 2-mercaptoethanol strong Pleurotus pulmonarius
1.10.3.2 EDTA slight Pleurotus pulmonarius
1.10.3.2 EGTA slight Pleurotus pulmonarius
1.10.3.2 Hg2+ strong Pleurotus pulmonarius
1.10.3.2 Mn2+ slight Pleurotus pulmonarius
1.10.3.2 Sodium azide strong Pleurotus pulmonarius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.012
-
 syringaldazine pH 6.5, 40°C Pleurotus pulmonarius
1.10.3.2 0.21
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 4.5, 40°C Pleurotus pulmonarius
1.10.3.2 0.55
-
 guaiacol pH 6.5, 40°C Pleurotus pulmonarius

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 copper Type I and type II copper center Pleurotus pulmonarius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 22400
-
 2 * 22400, SDS-PAGE Pleurotus pulmonarius
1.10.3.2 46000
-
 gel filtration Pleurotus pulmonarius

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.10.3.2 Acetone retains high degree of activity Pleurotus pulmonarius
1.10.3.2 acetonitrile retains high degree of activity Pleurotus pulmonarius

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Pleurotus pulmonarius
-
 main isoform Lcc2
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.10.3.2 glycoprotein 44% glycosylation Pleurotus pulmonarius

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 isoform Lcc2 Pleurotus pulmonarius

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 19750
-
 pH 6.5, 40°C Pleurotus pulmonarius

Storage Stability

EC Number Storage Stability Organism
1.10.3.2 4°C, pH 4.5-8.0, stable for several days Pleurotus pulmonarius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Pleurotus pulmonarius ? + H2O
-
 ?
1.10.3.2 guaiacol + O2
-
 Pleurotus pulmonarius ? + H2O
-
 ?
1.10.3.2 additional information no substrate: caffeic acid, ferulic acid, tyrosine Pleurotus pulmonarius ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Pleurotus pulmonarius ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 dimer 2 * 22400, SDS-PAGE Pleurotus pulmonarius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 50
-
-
 Pleurotus pulmonarius

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 50
-
 210 min, 50% residual activity Pleurotus pulmonarius
1.10.3.2 60
-
 23 min, 50% residual activity Pleurotus pulmonarius

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 310
-
 guaiacol pH 6.5, 40°C Pleurotus pulmonarius
1.10.3.2 654
-
 syringaldazine pH 6.5, 40°C Pleurotus pulmonarius
1.10.3.2 1520
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 4.5, 40°C Pleurotus pulmonarius

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 4 5.5 substrate 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Pleurotus pulmonarius
1.10.3.2 6 8 substrate guaiacol Pleurotus pulmonarius
1.10.3.2 6.2 6.5 substrate syringaldazine Pleurotus pulmonarius

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 4.5 8
-
 Pleurotus pulmonarius