EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.1.34 | DNA sequence determination and analysis, overexpression in Escherichia coli | Thermus thermophilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.34 | 23000 | - |
x * 23000, recombinant enzyme, SDS-PAGE | Thermus thermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.34 | Thermus thermophilus | - |
gene DHTt | - |
1.5.1.34 | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | - |
gene DHTt | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.34 | recombinant enzyme, partially from Escherichia coli, solubilization at pH 10.0 | Thermus thermophilus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.1.34 | 5.2 | - |
recombinant enzyme, substrate dihydrofolate | Thermus thermophilus |
1.5.1.34 | 26.2 | - |
recombinant enzyme, substrate 6,7-dimethyl-7,8-dihydropteridine | Thermus thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.34 | 6,7-dimethyl-7,8-dihydropteridine + NADPH | - |
Thermus thermophilus | (6R)-L-erythro-6,7-dimethyl-5,6,7,8-tetrahydrobiopterin + NADP+ | - |
? | |
1.5.1.34 | 6,7-dimethyl-7,8-dihydropteridine + NADPH | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | (6R)-L-erythro-6,7-dimethyl-5,6,7,8-tetrahydrobiopterin + NADP+ | - |
? | |
1.5.1.34 | dihydrofolate + NADPH | - |
Thermus thermophilus | tetrahydrofolate + NADP+ | - |
? | |
1.5.1.34 | dihydrofolate + NADPH | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | tetrahydrofolate + NADP+ | - |
? | |
1.5.1.34 | additional information | no activity with potassium ferricyanide, 6-biopterin, and 6-methyl-7,8-dihydropterine | Thermus thermophilus | ? | - |
? | |
1.5.1.34 | additional information | no activity with potassium ferricyanide, 6-biopterin, and 6-methyl-7,8-dihydropterine | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.34 | oligomer | x * 23000, recombinant enzyme, SDS-PAGE | Thermus thermophilus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.1.34 | DHPR | - |
Thermus thermophilus |
1.5.1.34 | dihydropteridine reductase | - |
Thermus thermophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.34 | 35 | - |
assay at | Thermus thermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.34 | 7.5 | - |
assay at | Thermus thermophilus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.34 | 5.5 | 8.5 | - |
Thermus thermophilus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.34 | additional information | no flavin prosthetic group | Thermus thermophilus | |
1.5.1.34 | NADPH | - |
Thermus thermophilus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.5.1.34 | Thermus thermophilus | isoelectric focusing | 4.7 | 4.6 |