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Literature summary extracted from

  • Duffner, F.; Bertoldo, C.; Andersen, J.T.; Wagner, K.; Antranikian, G.
    A new thermoactive pullulanase from Desulfurococcus mucosus: Cloning, sequencing, purification, and characterization of the recombinant enzyme after expression in Bacillus subtilis (2000), J. Bacteriol., 182, 6331-6338.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.41
-
 Desulfurococcus mucosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.41 ammonium sulfate 0.5 mM, 36% residual activity Desulfurococcus mucosus
3.2.1.41 CuCl2 5 mM, no residual activity Desulfurococcus mucosus
3.2.1.41 EDTA 5 mM, 63% residual activity Desulfurococcus mucosus
3.2.1.41 additional information not inhibitory: iodoacetamide, Triton X-100, Mn2+, Mg2+, Ca2+ Desulfurococcus mucosus
3.2.1.41 N-bromosuccinimide 0.01%, no residual activity Desulfurococcus mucosus
3.2.1.41 sodium dodecylsulfate 1 mM, no residual activity Desulfurococcus mucosus
3.2.1.41 Urea 4 M, 81% residual activity Desulfurococcus mucosus
3.2.1.41 ZnCl2 5 mM, 51% residual activity Desulfurococcus mucosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.41 74000
-
 x * 74000, deduced from gene sequence, mature protein Desulfurococcus mucosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.41 Desulfurococcus mucosus Q9HHB0
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.41 proteolytic modification enzyme contains signal sequence Desulfurococcus mucosus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.41 expressed in Bacillus subtilis Desulfurococcus mucosus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.41 culture fluid
-
 Desulfurococcus mucosus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.41 26
-
 pH 5.5, 80°C Desulfurococcus mucosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.41 amylopectin + H2O 35% of activity compared to pullulan Desulfurococcus mucosus ?
-
 ?
3.2.1.41 amylose + H2O 77% of activity compared to pullulan Desulfurococcus mucosus ?
-
 ?
3.2.1.41 beta-cyclodextrin + H2O 21% of activity compared to pullulan Desulfurococcus mucosus ?
-
 ?
3.2.1.41 gamma-cyclodextrin + H2O 71% of activity compared to pullulan Desulfurococcus mucosus ?
-
 ?
3.2.1.41 pullulan + H2O
-
 Desulfurococcus mucosus ?
-
 ?
3.2.1.41 starch + H2O 62% of activity compared to pullulan Desulfurococcus mucosus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.41 ? x * 74000, deduced from gene sequence, mature protein Desulfurococcus mucosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.41 85
-
-
 Desulfurococcus mucosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.41 75
-
 1 h, stable Desulfurococcus mucosus
3.2.1.41 80
-
 1 h, 90% residual activity Desulfurococcus mucosus
3.2.1.41 85
-
 1 h, 45% residual activity Desulfurococcus mucosus
3.2.1.41 90
-
 1 h, no residual activity Desulfurococcus mucosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.41 5
-
-
 Desulfurococcus mucosus